细胞

Studies on mouse nuclear transfer with adult male mouse ear fibroblast cells as donors In this study, C57BL/6 adult male mouse ear fibroblast cells and Kunming mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different passages of donor cells and electrofusion times on the in vitro development of NT reconstructed embryos, when the oocytes were treated with 3% sucrose.

以成年雄鼠耳成纤维细胞为供体克隆小鼠的研究该研究以C57BL/6 成年雄鼠耳成纤维细胞为供体，昆明白小鼠MⅡ期卵母细胞为受体，研究了3%蔗糖处理卵母细胞去核后，供体细胞不同传代次数和电融合次数对核移植重构胚体外发育的影响。

By suspending cell culture from the callus of P. thugbergii and by fluorescent observation, a new method, which is easy and sensitive to observe cell activity and to test the phytotoxicity of various bacteria, was used. This method can be utilized to study the bacterial toxins. Cultured filtrates of some strains of the nematode-carrying bacteria were bioassayed for their toxicity to suspending cultured cells from P. thugbergii callus. Strong toxic effects were observed by the filtrates of Pseudomonas spp., P. fluorescence, and Sphingomonas spp., these bacteria species are coincide with those in the early stage of pine wilt disease.

本试验使用了黑松愈伤组织的细胞悬浮培养方法，用荧光方法观察各种细菌对植物细胞的毒性测定，这种方法具对细胞的活性观察容易、灵敏度高等特点，证明可用于细菌的毒素研究；对松材线虫携带的细菌的部分菌株，用其培养滤液对黑松愈伤组织的悬浮培养细胞的生物测定初步证明，松材线虫携带的细菌中以假单胞菌、荧光假单胞菌和少动鞘氨醇单胞菌的滤液对黑松细胞具有较强的毒性，这与松树发病早期出现的细菌种类相一致。

Result: With a high cancer-inhibiting rate on the animal model having been transplanted the cancer, this medicine can remarkably restrain the growth of cancer cells cultivated in vitro and improve the life quality of mouse model, and also shows obvious promotion and enhancement function on the DTH ear-swelling of mouse with cancer, the proliferation of mouse spleen lymphocyte induced by ConA and the cytokine (TNF-α、IFN-γ) of mouse with cancer respectively. It can obviously decrease the adverse effects like weight reduction, damage of viscera and degradation of life quality caused by the chemotherapeutic medicines, and has a remarkable stimulation on the lymphocyte proliferation induced by ConA. It is also able to improve the ratio of SMMC-7721 cells in G0-G1 period and reduce the cell amount in G2/M period to cut down the possibility of stepping into the division period; additionally, it can obviously inhibit some cancer genes of the cancer cells and meanwhile up regulate the cancer-inhibiting gene.

结果：本品能明显抑制体外培养肿瘤细胞的生长，对在体肿瘤移植模型动物具有较高的抑瘤率，能明显提高模型小鼠的生存质量；对荷瘤小鼠DTH耳肿胀、ConA诱导的小鼠脾淋巴细胞增殖、荷瘤小鼠细胞因子（TNF-α、IFN-γ）呈现明显的促进或提高作用；能显著减少化疗药所引起的小鼠体重降低、脏器损害以及生存质量下降等不良作用；本品含药血清对ConA诱导的淋巴细胞增殖具有明显的促进作用；能提高SMMC-7721细胞G0-G1期比例，降低G2/M期细胞数目，而减少其进入分裂期；对肿瘤细胞的多个癌基因具有明显的抑制作用，同时上调抑癌基因等。

Result vitamin A and 4HPR fall to Hela cell collect form have apparent inhibition, can restrain Hela cell to be opposite the action causing tumor of naked rat (the curb tumour action of P.01); vitamin A issues visible cell solid by force to shrink at 4HPR(P.01); report lens, nuclear film is screwy, nuclear chromosome gathers agglomerate stand by the wither such as nuclear film to die DNA of cell of cellular feature; by degradation, a collection of illustrative plates of typical " of shape of " a flight of stairs appears in electrophoresis of agaragar candy gel.

结果维生素A和4HPR对Hela细胞集落形成有明显抑制功能，并可抑制Hela细胞对裸鼠的致瘤功能(P.01)；维生素A的抑瘤功能强于4HPR(P.01)；电镜下可见细胞固缩，核膜扭曲，核染色体聚集成块并靠近核膜等凋亡细胞特征；细胞DNA被降解，在琼脂糖凝胶电泳中呈现典型的&阶梯状&图谱。

Second, because the thoracic duct drainage can be particularly lymphocytes from the large number of T cells in vitro, to reduce its dermal layer deposition, as well as the release of lymphokines, resulting in T-cell subsets change, helper T cells and the inhibition of T-cells ratio decreased the humoral immune function, B cells to produce specific antibodies decreased.

二是因为胸导管引流术可以把淋巴细胞尤其是T细胞大量排出体外，减少其在真皮层内的沉积，以及淋巴因子的释放，从而导致T细胞亚群的改变，辅助性T细胞与抑制性T细胞比值下降，体液免疫功能改变，B细胞产生特异性抗体能力下降。

Objective: Comparative and extended research among the newly found cell, microglia cell and atypical cell in the rat organ of Corti after aminoglycoside intoxication; to further explore quality, origin, and the relationship between the newly found cell and the neural stem cell or nestin progenitor cell.

目的：对大鼠耳蜗药物损伤后出现的新细胞与小胶质细胞、非典型细胞进行比较和扩展研究，探讨新细胞的代表性及其起源、转归、以及与神经干细胞或nestin前体细胞的关系。

At the same time, group B cells were sequential planted by allowing cells to adhere to tissue culture dishes during two successive 2-hour incubations in order to remove non-adherent cells.

分别采用常规方法和序列黏附法培养。A组细胞于接种第4天洗去未黏附细胞，然后隔d换液1次；B组细胞在接种后每2h去除1次未黏附细胞，共2次。2组细胞均在第7天计数早期克隆，持续培养直到晚期克隆出现。

He expression of TLR4 is also increased in the peripheral blood monocytes from the patients with ACS.

LR4在动脉粥样斑块的内皮细胞、巨噬细胞、血管平滑肌细胞和血管外膜成纤维细胞表达均增高，在急性冠脉综合征患者外周血单核细胞表达增高。

However, when two maturing oocytes fused and two spindle will form in the big cell, the chromosomes will not intermingle. In the present study, we removed GV from one oocyte and transfer to the perivetelline space of another GV oocyte. After fusion the resulting oocytes which contained two GVs were cultured further in MEM.

在本实验中，我们利用小鼠GV期卵母细胞将一枚卵母细胞的生发泡取出后移至另一未经去核处理的GV期卵母细胞透明带下，经三次电脉冲作用后将融合的含有两个GV的卵母细胞放入MEM成熟培养液中培养，在培养成熟的不同阶段分别收集卵母细胞进行免疫荧光染色观察微管及核的变化。

The results showed that after 2 h of culture in MEM the chromosomes of oocyte were seperated to form telophase I while a small spindle was observed around chromosomes of primary spermatocyte. However, two clear spindles were observed in the oocytes cultured in CB containing MEM. After further culture, the chromosomes of both primary spermatocyte and oocyte intermingled and formed one large spindle.

在无CB的培养液中培养的卵母细胞培养2小时后，卵母细胞已经进入第一次减数分裂的后期，染色体开始被拉向两极，而精母细胞的MI纺锤体才刚刚形成，虽然继续培养两者染色体可以合二为一并形成一个纺锤体，但是有些染色体发生滞后；当卵母细胞在含有CB的培养液中培养2小时后，在卵母细胞内形成两个相似大小的MI纺锤体，进一步培养形成一个大的纺锤体，染色体正常。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。