细胞

The status of cell growth was observed under inverted microscope and the morphological characters were studied with the cells stained by Wrights staining .

倒置显微镜动态观察细胞生长情况，瑞氏染色观察细胞形态特征，并采用细胞化学和免疫细胞化学方法对获得的细胞进行鉴定。

RESULTS: In the verapamil group, the fibroblast were round or oval, and there were tiny cell process, less alpha substance, atrophic and decreased rough endoplasmic reticulum in kytoplasm and abundant vesica. In control group, there were fusiform or polygon fibroblast, and much matrix among cells, abundant rough endoplasmic reticulum and ribosome in cytoplasm and less vesica were found.

结果：维拉帕米组神经瘢痕成纤维细胞呈球形或椭圆形，细胞突起细小，胞间网状物质少，胞浆中粗面内质网萎缩且数量减少，囊泡丰富；对照组成纤维细胞呈梭形或多角形，细胞间基质较多，细胞浆内粗面内质网及核糖体丰富，囊泡少见。

There was significant difference in signal intensity between the cell concentration group and unlabelled cell group during 2 weeks. And significant difference occurred in the 5×105 SPIO-labeled cells per milliliter medium group and unlabelled cell group during 3 weeks.3. In kidneys of ischemia-reperfusion injury,the labeled BMSCs were demonstrated as signal intensity loss in renal medulla on T2*WI sequence in days 1,3,and5.The signal intensity loss was visualized up to 8 days after transplantation,and the signal intensity in the third day was the lowest. Histological analyses showed that most Prussian blue staining-positive cells were well correlated with the area where a signal intensity loss was observed in MRI.

在此细胞浓度的基础上，标记后细胞信号在T2*WI序列的降低在2w后失去统计学意义；而在细胞浓度为5×105/ml时，标记3w后信号在T2*WI序列的降低才失去统计学意义。3缺血再灌注肾损伤家兔细胞移植后第1、3、5天肾脏髓质区信号强度明显下降，至移植后第8天信号改变不明显，其中第3天信号改变最为明显；组织学分析见绝大多数普鲁士蓝染阳性标记细胞分布于肾小管周围，与MRI信号强度下降区域基本一致。

By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中，经G418筛选培养20天后再行Southern杂交检测外源〓整合情况，利用RNA狭线杂交法证明了在转录水平上表达有所提高，进一步进行了流式细胞仪测定DNA含量，使细胞在〓期产生生长阻滞作用，细胞生长速度，在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化，我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

Objective To establish a competitive RT-PCR method for quantitative determination of IFN-γ mRNA and IL-4 mRNA and explore the optimal inductive condition in vitro. Methods IFN-γ mRNA and IL-4 mRNA in PBMC induced by PMA and calcium ionophore of a healthy volunteer and in unstimulated PBMC of 11 HVs and 13 asthma patients were determined quantitatively with the established competitive RT-PCR method using self-prepared internal standard as competitive templets.

Subject words 】 Competitive templet； IFN-γ； IL-4； mRNA； RT-PCR 检测细胞因子最早使用生物学或免疫学测定法，这两类检测可反映某种细胞因子分泌、吸附、消耗和降解后的净含量〔1〕，但不能为细胞因子产生过程中基因转录、翻译及翻译后调控机制提供必要的信息，也不能反映细胞或组织内细胞因子基因表达水平。

The proliferative capacity of LEC from human had directly relevance to the age of donor(r=-0.996).Conclusions The formation of lens-like spherules is characteristics of LEC lines in the cultured cells.Under identical conditions,the proliferative rate of LEC from bovine and rabbit is fast than that of LEC from human,but dedifferentiation of LEC from bovine and rabbit is easier than that of LEC from human;LEC from the three species exhibit similar limited growth potential.The proliferative rate of LEC from human has a inversely proportion with age.

&晶状体小体&的形成可作为确定晶状体上皮细胞株的一项特征性依据，而体外培养的人、牛、兔晶状体上皮细胞具有相同的有限生长潜能，在相同的条件下，牛、兔晶状体上皮细胞的生长增殖速度比人晶状体上皮细胞快，但易于发生去分化；此外，人晶状体上皮细胞的生长增殖率与年龄密切相关，年龄越小，晶状体上皮细胞的生长增殖速度越快。

Results In spheroids culture system, under the condition of apoptosis of src-transformed cells, the apoptosis of chemical transformed cells were also induced by normal cells, but BPV-transformed cells were resistant.

结果：在球体细胞培养实验系统中，在src转化细胞产生凋亡的条件下，甲基胆蒽转化细胞同样能产生凋亡，而BPV转化细胞则抵抗了由周围正常细胞所致的凋亡。

Specific inhibition of Hh signalling using small molecule inhibitors, a neutralizing anti-Hh antibody or genetic deletion of smoothened in the mouse stroma results in growth inhibition in xenograft tumour models.

由癌细胞分泌的Hedgehog配体，在肿瘤表皮细胞中未能激活信号作用，而是在&基质&上发挥作用（&基质&由细胞外基质、成纤维细胞、内皮细胞和微血管组成，恶性细胞着床于其中）。

The two can cause ultrastructural pathogenesis widely in many organs and tissues such as hepar, pancreas, lien, ren, pulmo, cerebral, cerebellum, cor, muscular and so on, of them ,caused by i~olates YG97 and Y98 more obvious in epithelial cells of digestive tract and unobvious in epithelial cells of respiratory tract ,but ultrastructural pathogenesis caused by NDV strain F48E8 ,more obvious and more serious in glandularis and muscularis ventriaculi and in epithelial cells of respiratory tract .

将鹅源分离株YG_(97)、鸡源分离株Y_(98)和NDV强毒株F_(48)E_8感染SPF鸡，运用扫描电镜和透射电镜观察该二株病毒对机体细胞的影响，结果显示三株禽副粘病毒均引起机体肝、胰、脾、肾、肺、小脑、大脑、心和肌肉等实质器官组织细胞的超微病变，其中YG_(97)和Y_(98)引起消化道粘膜上皮细胞发生微绒毛脱落、纤毛脱落、分泌颗粒增多、细胞游离面破溃等显著的超微病变，引起气管粘膜上皮细胞的不显著超微病变；F_(48)E_8强毒株引起胃粘膜显著而严重的超微病变；还引起气管粘膜上皮严重的超微病变。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。