细胞

The degree of destruction of these three target cells correlated well to cell sensitivity of virus multiplication and amount of virus antigen on cell membrane. By means o...

在上述破坏细胞过程中，加入抗球蛋白，对地鼠肾细胞感染病毒的早期有促进靶细胞破坏的作用，对HeLa细胞不显，对鼻咽癌细胞则无。

after treated with glu (5 mmol/l) for 24 h, the viability of pc12 cells depressed and the viable cells was only 58.3%when compared with cell control group tested by mtt assay.

体外培养pc12细胞，建立谷氨酸诱导pc12细胞损伤的模型；采用mtt法检测细胞活力，流式细胞术和hoechst 33258 dna 染色法检测细胞凋亡。

Much progress has been made in recent years through studying septic shock,however brain injury is rarely involved.This study takes the models of endotoxin-shock rats as research object to simulate septic shock.The study aims are to explore anomalous signal transduction in brain injury caused by septic shock and the effect of Sini Decoction in the process;define the molecular and cellular mechanisms of reviving yang for resuscitation method used to prevent and treat brain injury in septic shock and establish the scientific foundation for modernizing traditional Chinese medicine.

研究目的：1。明确内毒素休克后脑损伤组织中炎症介质水平，中性粒细胞和脑血管内皮细胞等细胞表面黏附分子表达情况，脑血管平滑肌细胞、神经胶质细胞和神经细胞对细胞外炎症介质刺激产生的功能代谢变化以及结构改变等三者之间的规律性关系。2。

METHODS: SGC7901 cells were transfected with siRNAs targeted against the human PLK1 by chemosynthesis. The PLK1 mRNA levels of cells transfected with siRNAs were monitored by real time PCR and the protein levels were detected by immunocytochemistry. Cell proliferation was evaluated by direct cell counting with trypan blue staining.

化学合成针对PLK1的小干扰RNA，转染至胃癌细胞株SGC7901中，利用实时定量PCR、免疫细胞化学、台盼蓝活性细胞计数和流式细胞术对PLK1的基因表达、细胞增殖、细胞周期和凋亡的检测。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

METHODS: Spleen cells of 1, 2, 4, 6, 8, 10, 12, 16, 20 and 24 h in the coculture system were observed and the expression of RNA editase was detected by semiquantitative PCR and was contrasted with the mouse spleen cells in a singleculture system. The mouse model with the immune system restrained by FK506 was made, its spleen cells were cocultured with stimulated cells and then the effect of FK506 on RNA expression of editase was detected by semiquantitative PCR.

观察共培养1，2，4，6，8，10，12，16，20及24 h的小鼠脾细胞，用半定量PCR的方法检测RNA编辑酶表达的变化，并以相同时段单独培养的小鼠脾细胞RNA编辑酶的表达情况进行对照；制备免疫功能受FK506抑制的小鼠模型，取其脾细胞与刺激细胞进行相同时段的共培养，用半定量PCR的方法检测FK506对脾细胞RNA编辑酶的影响。

After renal cortexes were Grinded and digested with 0.25%tryspase.The cell types of epithelial cell were identified by immunocytochemistry.The injury model of rats, renal tubular epithelial cells were established by using gentamicin.And observed the role of bFGF in renal tubular epithelial cell.

通过对大鼠肾皮质研磨、过网的方法，并以0.25%胰蛋白酶消化，以免疫细胞化学方法鉴定体外培养的肾小管上皮细胞(抗Cytokeratin 18抗体)，以庆大霉素对培养的肾小管上皮细胞建立损害作用模型，然后观察碱性成纤维细胞生长因子对损害的肾小管上皮细胞的保护作用。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞，运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化；用gp120处理并用anti-gp120-FITC进行染色，运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况；用抗磷酸化ERK 抗体免疫荧光方法进行染色，运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

In vivo treated sera from ATG-treated patients produced positive test results for T CDC and T/B FXM, while the B-cell CDC crossmatch remained negative.

来自于ATG治疗的病人血清测试发现，引起T细胞的补体依赖细胞毒性反应和 T/B的流式细胞计数阳性结果相关，而同时，B细胞补体依赖细胞毒性交叉配血保持阴性。

Results: The positive results of in-situ hybridization showed that the lung tissues of all cases expressed SARS-CoV RNA, and positive signals displayed in cytoplasms （purple-blue, NBP-BCIP.

原位杂交和免疫组化结果显示：支气管上皮细胞、Ⅱ型肺泡上皮细胞、血管内皮细胞、巨噬细胞、纤维母细胞及T淋巴细胞在所有SARS病例中都受到了病毒感染。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。