细胞

On human carcinoma cells.MethodsMorphological change observation and live cell accounting were used to investigate the inhibitory effects of Sanguisorba officinalis L.

方法细胞形态观察和活细胞计数法研究地榆水提液对人白血病细胞K562，肝癌细胞HepG2，胃癌细胞BGC823，宫颈癌细胞Hela生长的抑制作用。

Desmin, the marker of myoblasts, was adopted to identify the expression of specific marker protein of sarcoblast desmin with immunohistochemistry. Desmin negative cell clones were removed, and desmin cell clones were cultured continuously with the culture fluid replaced once every other day.

采用成肌细胞特异性标志抗原desmin免疫化学染色，鉴定成肌细胞标志蛋白——结蛋白的表达，弃去desmin阴性的细胞克隆，继续培养desmin阳性的细胞克隆，隔天换液1次，7 d进行酶消化传代，获得大量扩增的细胞，并可冻存复苏，用于实验。

The result shows that the purification efficiencies of ammonia-nitrogen and ortho-phosphate by immobilized algae in a stationary phase are higher than that in an exponential phase; the starvation treatment increases the removing rates of ammonia-nitrogen and ortho-phosphate, cells of Scenedesmus starved for 48 hours can obtain higher NF4(superscript +)-N and PO(superscript 3- subscript 4)-P removal efficiencies than starved for 48, 72, 24 and 0 hours respectively.

结果表明：静止初期藻细胞对NH4-N和PO(上标 3-下标 4)-P去除率比指数末期高，处理5d后，静止初期细胞对NH4-N和PO(上标 3-下标 4)-P的去除率分别为76.7%及91.7%，指数末期细胞氮、磷的去除率分别为75.4%和88.7%；饥饿处理可明显提高藻细胞对NH4-N和PO(上标 3-下标 4)-P的去除效率，藻细胞饥饿时间对NH4-N和PO(上标 3-下标 4)-P去除率的影响大小依次为48h，72h，24h，0h。

After 5 days, we determined the effects of LF-PMF on EPCs' proliferation ability by MTT assay, cell cycle by flow cytometry, migration ability by cell scrath assay and angiogenic potential by tube formation assay and 3D- culture assay.

曝磁5天后，MTT法检测细胞增殖情况，流式细胞仪检测细胞周期，划痕试验检测细胞迁移能力，管状结构形成试验和三维培养检测细胞成血管能力。

If GVHD occurred, GCV would be administered in order to kill TK〓T lymphocytes selectedly and to control GVHD, while preserving therapeutic effects of T lymphocytes.

将TK基因体外转染供者T细胞成为TK〓T细胞，然后和CD34〓细胞混合移植，一旦发生GVHD，则给予特异性药物丙氧鸟苷治疗，选择性杀死引起GVHD的TK〓T细胞，使GVHD得到控制，同时又可保留T细胞的治疗作用。

Results: Follow-up ob servation of living cells revealed that upon the Ti disc there were cells migra ting from the cellular layer and attaching to the rim of it, forming cells bridg es between them, and then more cells were found to attach and orient as the cult ure went on.

结果：活细胞跟踪观察揭示钛片放入细胞层表面后即有细胞自细胞层表面向钛片侧壁移行、贴附，并在二者之间形成细胞桥。

Results: Follow-up ob servation of living cells revealed that upon the Ti disc there were cells migra ting from the cellular layer and attaching to the rim of it, forming cells bridg es between them, and then more cells were found to attach and orient as the cult ure wen

结果：活细胞跟踪观察揭示钛片放入细胞层表面后即有细胞自细胞层表面向钛片侧壁移行、贴附，并在二者之间形成细胞桥。

Methods : MTr assay was used to examine the effect of Sorbaria sorbifolia on the proliferation of HepG-2 cell and flow cytometry was used for the cell cycle distribution.

采用MTT比色法观察珍珠梅提取物抑制肝癌HepG-2细胞增殖；采用流式细胞仪检测其对HepG-2细胞周期分布的影响，同时结合倒置显微镜、HE染色、透射电镜及免疫细胞化学方法检测凋亡相关基因bcl-2、p53的蛋白表达，观察其诱导肝癌HepG-2细胞凋亡的作用。

Methods Hepatoma carcinoma cells and pulmonary carcinoma cells were cultured in vitro, Mononuclear cells in healthy person's peripheral blood were collected. PBMCs were mixed with liver cancer and lung cancer cells by proportion, and enriched tumor cells by superparamagnetic nanoparticle antibody complex, lung cancer specificness was identified by fluorescence nanocrystals bioprobe. We smeared tumor cells on glass slide and identified pulmonary carcinoma cells specfficity by fluorescence nanocrystals bioprobe.

体外培养人肝癌细胞和肺癌细胞，采集健康人外周血单个核细胞，将PBMC与肝癌细胞和肺癌细胞按比例混合，通过磁粒抗体复合物富集癌细胞，用荧光纳米晶探针进行肺癌特异性鉴定。

The HUVECs retained spindled shaped or cobblestone appearance and positively stained by anti-CD31. DAPI staining showed that 90％of scaffolds surface was reendothelialized and endothelial cells were distributed evently on the surface forming a confluent monolayer.

脱细胞支架细胞成分完全脱去，胞外基质保存完好；分离的细胞培养一周后，细胞呈梭形或多角形，核仁清晰，大小均匀，呈典型的铺路石样；免疫荧光检测可见细胞胞浆内有颗粒状红色阳性信号，说明Ⅷ因子相关抗原染色阳性；膜表面有蓝色阳性信号，说明CD31抗原染色阳性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。