细胞

The mature egg cell was an inactive cell with only a few polysomes. At the early zygote stage, a large number of ribosomal precursors were produced by the nucleolus, and many polysomes appeared in the cytoplasm, which suggests a high level of metabolism. Zygote at the dormancy stage had a small nucleolus and marked decrease in ribosomes, as shown by a few polysomes, which suggests decreased metabolism. Zygotes in the prophase of mitosis and two-celled proembryo became active again in metabolism, for a prominent nucleolus, high density of ribosomes and increased number of polysomes in the cytoplasm.

结果如下：在成熟卵细胞中多聚核糖体数量不多，且细胞代谢活性较弱；初期合子内，核仁大量合成核糖体前体物质，胞质中多聚核糖体数目众多，细胞代谢活性较强；休眠期合子的核仁变小，胞质中核糖体数量急剧减少，仅有少量多聚核糖体，细胞代谢活性较弱；合子分裂前期和二细胞原胚期，核仁显著，胞质中核糖体的密度增加，出现大量多聚核糖体，细胞代谢活性较强。

Cell cycle analysis was performed using flow cytometry and proliferous index was calculated.(9) Drug sensitivity assay was performed using MTT assay.

8借助流式细胞仪对基因转染细胞及其对照细胞进行细胞周期分析，并计算细胞的增殖指数(proliferous index，PI)。

To pronuclear formation rate after oocyte in vitro maturation 18 hours and parthenogenticactivation, oocytes derived from SM is significantly higher than oocytes from PM,oocytes derived from IM is significantly lower that of oocytes detrived from PM and SM.

对于孤雌刺激反应而言，无论是何种类型的卵母细胞成熟18小时后，自发成熟卵母细胞激活后形成原核的比率高于被动成熟的卵母细胞，诱导成熟的卵母细胞的原核形成率显著低于自发和／或被动成熟的卵母细胞。

Cyte including protoplasmic astrocyte and fibrous astrocyte, oligodendrocyte, microglia, and epen

细胞和纤维型星形胶质细胞，少突胶质细胞、小胶质细胞和室管膜细胞；在周围神经系统中

Examination the cell shape and pseudopodia with confocal laser scanning microscopy, scanning electron microscopy, and atomic force microscopy, we found that cell spreading well on nanogrooved substrates and their body was more flat and slim; meanwhile, on plain substrates, cells were mostly round.

纳米沟槽能很好地促进细胞贴壁黏附、铺展、生长和增殖，并影响细胞的周期；纳米沟槽诱导细胞骨架发生重组，肌动蛋白骨架沿着纳米沟槽的方向取向排列；纳米沟槽影响细胞伪足形成的方式和形成与细胞中的位置。

Results The cells became larger and showed more pseudopodia, having atendency to differentiate, the detection of cell cycle showed a decrease of S stage andan increase of G1 stare, cell proliferation ability in vitro and tumorigenesis ability in nude mice were discounted, the final tumor volume and weight were also decreasedafter knocking down NS gene.

结果NS基因在PC-3细胞中的表达水平下调后，细胞胞膜边缘突起增多，更趋向于分化；S期细胞的百分率降低，G1期的百分率升高；细胞的体外增殖速率明显降低，在裸鼠体内致瘤能力、肿瘤的生长速度及肿瘤的最终体积和重量也明显降低，细胞凋亡增多。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

The COX-2 positive cells were odontoblasts, pulpal fibroblasts vascularendothelial cells and macrophages (positive neutrophils could be found in some cases of the second group and of the third group).(3)We found that COX-2 developed up with HIF-1 a in third group ,but against it infourth group.

COX-2表达阳性的细胞有成牙本质细胞、成纤维细胞、血管内皮细胞和单核巨噬细胞(阳性中性粒细胞可出现在第二组和第三组)，HIF-1α与COX-2的表达相关主要在第三组和第四组牙髓标本中体现明显，在第三组二者的变化大致呈平行关系；在第四组基本呈反向变化关系。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养，细胞铺满培养瓶底后首次传代，通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化；通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况，对细胞进行鉴定。

In electricity plus thermal group, the pathologic alterations were slighter than model group; there was little crack on the surface of cartilage, with intact collagen fiber structure and less pyknotic chondrocytes. Although some chondrocytes shrank, there were intact cell organs in many chondrocytes even chondrocyte cluster in some regions.

疏密波+热软膜组在病理程度上的改变明显轻于模型对照组，软骨表面裂纹少，胶原纤维结构基本完整，固缩的软骨细胞少，虽可见部分退变的软骨细胞，但部分软骨细胞具有较多的细胞器，某些区域形成软骨细胞簇。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。