细胞

The toxicity and the osteogenous ability on the surface of PCHA: the toxicity was examined by coculture with PCHA to record growth of cells with MTT method. The osteogenous ability was investigated by observation of the formation and growth of cells on the surface of PCHA bone cement.

PCHA毒性检测和表面细胞活性的观察：将PCHA浸出液与骨髓基质细胞共同培养，通过MTT比色描记细胞生长曲线，快速判定制孔剂添加后PCHA的毒性反应；将PCHA制成标准试件，与骨髓基质细胞共同培养，观察细胞形态和生长活性。

RESULTS: High-purity ADSCs were acquired successfully. The ADSCs were induced into osteoplastic differentiation, and displayed typical adipocytes changes 7 days after induction. the cells turned to slender fusiform and mixed together, which showed positive PPARγ immunohistochemical staining and the lipid droplet was stained orange-red by oil red staining.

结果：①成功获得纯度较高的脂肪基质细胞，成功诱导其脂向分化，诱导培养后7 d观察到细胞形态学上表现出典型的脂肪细胞改变：细胞相互融合，成片生长，变为细长纺锤形；PPARγ免疫细胞化学染色呈阳性；油红O染色脂滴被染成橙红色。

The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P＜0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本试验推荐猪体外成熟培养体系为连续培养液48h的NCSU-23+10％FBS，细胞浓度为100个／400μl，发育培养体系为NCSU-23+BSA；用CRY-3细胞激活仪电激活时(电融槽宽度为1mm)为150v／20μs／2次+600μMγ-BLI；以活力为0.1左右的冷冻精液进行IVF时，精子浓度为10~7，共孵时间为6h，并在成熟处理时只部分去除卵丘细胞；MⅡ卵母细胞冷冻时，方法为移液枪头法+离心法，冷冻后激活的2-细胞卵裂率比IVF高。

Brane, such as synoviocytes, macrophages, osteoclasts and TNF-αvv acts on different kinds of cell in synovial memchondrocytes, which can produce metalloproteinase,collagenase, stromelysin and so on, further induce pannus formation, joint inflammation, bone erosion and cartilage degradation.

TNF-α可作用于RA关节组织中的滑膜细胞、巨噬细胞、软骨细胞和破骨细胞，使这些细胞活化，产生金属蛋白酶、胶原酶、基膜溶解酶等，导致局部炎症反应和血管翳形成，进一步引起软骨破坏和骨侵蚀。

Following small injections into the rostral part (namely the dysgranularfield, DI) of the insulpr cortex, retrogradely labeled neuronswere primarily in the parvicellular ventroposterior medial nucleus of the thalamus, the "waist" area of the PBN.

标记细胞有卵圆形、梭形和不规则形，大小平均15u。将HRP注入岛皮质后都，标记细胞见于VPLpc和PBN：VPLpc的标记细胞多位于其中间部，密度较低，以圆形卵圆形细胞为主，梭形和三角形细胞较少见。

Methods K-ras gene point mutation and its style at codon 12 of Patu 8988 cell were detected by using PCR-SSP and sequence analysis.

方法顺序特异引物聚合酶链反应法和基因测序检测Patu8988细胞K-ras点突变形式，根据点突变形式设计并合成硫代反义寡核苷酸（K-ras mutation ASODN）作用Patu 8988，通过噻唑蓝比色法检测细胞生长情况；流式细胞术检测K-ras蛋白表达和细胞凋亡；逆转录-聚合酶链反应检测K-ras mRNA表达水平；以Patu8988细胞建立裸鼠胰腺癌模型观察K-ras mutation ASODN在体内的抗肿瘤效果。

The results of the ultrastructares of the glandular hairs show that there are a number of the plastids in which there are a big of osmiophilic substances in the secretory cells of the peltate hairs and then that there are much endoplasmic reticulum in the secretory cells of head hairs. The difference between them are with relation to the function.

腺毛超微结构观察的结果表明，盾状腺毛类分泌细胞的优势细胞器是质体，其中具大量的嗜锇物质；而头状腺毛类分泌细胞的优势细胞器是内质网，这种细胞器上的差异与头状腺毛和盾状腺毛的分泌功能是相适应的。

At early stage of cancer cell formation, provoked autophagy help to eliminate damaged cell organelle、and degrade the noxious substance in endochylema(eg: peroxidate)as well, pretect normal cells from cancerization.Autophagy level modulation might be cooperation or rivalry with chemotherapy to cancer cells compares to single use of chemotherapy drugs, apoptosis rate of cancer cells fluctuates with autophagy level.

癌细胞形成早期刺激自噬能够清除受损细胞器，分解细胞内过多的有害物质，阻止正常细胞向癌细胞转化，调控自噬的表达和化疗药物联合对肿瘤细胞的生长有协同或拮抗作用，表现为与单纯用化疗药物相比，调控自噬表达后肿瘤细胞的凋亡率发生了变化。

Monocytes generated from bone marrow of Balb/cj mouse were cultured for 6 days in complete RPMI 1640 medium containing 10％ FBS, rmGM-CSF and rmIL-4.50 mg·L-1 acteoside or isoacteoside was added to cells on day 6 of culture for 24 h.The surface molecules expression level of DCs and their phagocytose ability were analysis by flow cytometry.

采用细胞因子诱导法，从Balb/cj小鼠骨髓细胞贴壁分离获得单核细胞，加入含10%胎牛血清、10 μg·L-1重组小鼠粒细胞巨噬细胞集落刺激因子及重组小鼠白细胞介素-4(rmIL-4)的RPMI 1640完全培养基培养，在培养第6天，实验组加入车前子苯乙醇苷类化合物(10，50，100 mg·L-1)，对照组加入RPMI 1640或LPS(1 mg·L-1)，流式细胞仪检测DCs表面分子CD11c，CD86，MHC II和CD80的表达以及各组DCs吞噬功能的变化。

Reverse-transcription PCR assays were used to detect the mRNA expression level of related regulatory genes such as p15, p16, p21, p27, p57, surviving, cyclin B, cdc2 and chk1, and Western blot assay to detect the level of protein expression and phosphated change such as survivin, cdc2 and chk1. Results:(1) K562 cell arrested on G2/M phase were obviously increased after co-cultured with 2 to 10μmol/L Arsenic trioxide for 24 hours. The ratios of control group, 2μmol/L, 5μmol/L and 10μmol/L groups were 22.6±3.4%, 27.2±2.3%, 43.8±4.5% and 36.7±4.1%, respectively.

体外培养K562细胞，以不同浓度AS_2O_3作用不同时间后采用PI染色、流式细胞仪检测药物作用后细胞的周期分布改变；逆转录酶多聚酶链扩增方法检测细胞周期相关调节基因(p15、p16、p21、p27、p57、survivin、cyclinB、cdc2、chk1)的mRNA表达变化；Western blot方法检测细胞周期相关调节蛋白表达及磷酸化改变(survivin、cdc2、cdc2-p、chk1)。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。