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The ameloblasts are the key cells during odontogenesis, and they have been already cultured in vitro. The amelogenin is the extracellular matrix synthesized and secreted by the ameloblasts, and it plays a crucial role during amelogenesis.

成釉细胞是牙器官形成的关键细胞,国内外已成功离体培养出成釉细胞,成釉细胞合成和分泌的细胞外基质-釉原蛋白在釉质的形成中起着关键作用。

When induced to differentiate in the presence of Oleaceae oil (35-130 mg/ml), 3T3-L1 preadipocytes dose- dependently reduced their ability to accumulate cellular triglycerides. Cytoplasmic triglyceride droplets could also be visualized by microscopic observation. Cells treated with 130 mg/ml Oleaceae oil retained their morphology as spindly fibroblasts, while normal adipocytes take on a rounded shape filled with triglyceride droplets.

在开始刺激细胞分化的同时,以 35 mg/ml 到 130 mg/ml 不同的木樨科精油浓度处理细胞,为期八天,将分化到第八天的细胞收下来测三酸甘油酯的浓度当作分化程度的指标,结果发现随著木樨科精油浓度的增加,细胞所累积的三酸甘油酯浓度就越低,用显微镜观察即可发现细胞中脂肪油滴所占的比例明显降低。

Oligodenderocyte and oligodendroglioma cells in vitro were able to secret beta-trace protein and explained why the concentration of β-tp in CSF was higher than that in serum, it was suggested that the level of β-tp released depended on both of cell member and type.

胶质细胞和人脑胶质瘤细胞能分泌β-tp,细胞分泌β-tp水平的高低由细胞类型及细胞的多寡决定。

Results: the findings on ct and mri were as follows: 57 cases of oligodendroglioma, 15 cases of ganglioglioma , 5 cases of dysembryoplastic neuroepithelial tumor , 3 cases of pleomorphic xanthoastrocytoma .conclusion:the images on ct and mri of cerebra peripheral tumor occur some characters , so ct and mri were certain worth on the diagnose and differentiate diagnose of cerebra peripheral tumor.

结果:少枝胶质瘤47例,间变性少枝胶质瘤10例,节细胞胶质瘤15例,胚胎发育不良性神经上皮瘤5例,多形黄色细胞瘤3例。结论:脑浅表肿瘤存在影像特征,ct及mri对其诊断有一定的价值。少枝胶质细胞瘤;节细胞肿瘤;胚胎发育不良性神经上皮瘤;多形性黄色星形细胞瘤;ct;mri

About 48%(90/194) were below 29 years of age. Ninety-nine of 194 cases had either radiotherapy alone or supplementary treatment with radiotherapy following surgery. We analyzed multiple prognostic factors. The factors of importance included postoperative radiotherapy; female in glioblastoma multiforme; the primary tumor dose above 5000 rads and/or whole brain irradiation in malignant astrocytoma and glioblastoma multiforme; the primary tumor dose above 4500 rads in ependymoma, medulloblastoma and oligodendroglioma; and spinal axis irradiation above 2000 rads in ependymoma and medulloblastoma that produced a better prognosis than in other groups.

男与女之比例为2.7比1,年龄分布自1至81岁,平均年龄33.3±19.3岁,中值年龄为34岁,29岁以下占48%(90/194),其中99例单独或手术后辅以放射腺治疗,分析同种脑瘤的预后因素,其中多形神经胶母细胞瘤有手术后放射线治疗或女性,多形神经胶母细胞瘤合并星细胞瘤原发肿瘤剂量5000雷得以上或曾全颅腔照射,神经管胚细胞瘤,间胶质母细胞瘤,及室管膜瘤原发肿瘤剂量4500雷得以上,室管膜瘤及神经管胚细胞瘤有脊轴照射2000雷得以上预后均佳,且统计上有显著的差异。

Primary culture of bovine corneal endothelial cells and whole rabbit corneas were used as the experimental materials. We first treated the cultured bovine corneal endothelial cells with PTPs inhibitor, sodium orthovanadate, with a variety of concentrations (25,50,100μM) for various durations (8,24 hrs). The effects of PTP inhibition on cellular distribution of cell-cell junctional proteins, such as N-cadherin, alpha-catenin and p120, were evaluated by immunohistochemical staining with fluorescein microscopy and confocal microscopy. Immunohistochemical staining with Ki67 Ab, a marker of cell proliferation, was also used to detect cells entering cell cycle.

实验材料为初代培养之牛角膜内皮细胞和全厚度之兔子眼角膜,使用sodium orthovanadate作为酪胺酸磷酸脢的抑制剂,接著以不同SOV浓度(25, 50, 100μM)及不同时间(8, 24 hrs)来处理初代培养之细胞与新鲜的兔子眼角膜,之后进行萤光染色并使用萤光显微镜及共轭焦显微镜进行影像撷取,以观察位於细胞细胞间的蛋白质,例如:N-cadherin, alpha-catenin 和p120的改变,另外也使用Ki67抗体来侦测是否有进入cell cycle的细胞

Emerging evidence indicates that both osteoclastic activity and osteoblastic activity are required for the development of prostate cancer bone metastasis.

愈来愈多的证据显示,破骨细胞和成骨细胞共同参与了前列腺癌的骨转移,调节破骨细胞和成骨细胞细胞因子影响前列腺癌细胞的发展与转移。

This study is to further investigate the mechanisms of osteoclastic bone resorption induced by IL-1 with established osteoclasts culture and osteoclasts, bone marrow stromal cells co-culture system.

本文利用建立的破骨细胞培养和破骨细胞、骨髓基质细胞共培养体系,在体外进一步研究IL-1诱导破骨细胞性骨吸收的机制,旨在探讨细胞因子在骨质疏松症骨丢失发生中的作用。

The effect of PTH on the osteoclastic bone resorption,and how the osteo-blasts mediat the reaction was studied in vitro by using the method of isolating and cul-turing rabbit osteoclasts and osteoblasts.

采用分离、培养兔破骨细胞和成骨细胞的方法,体外研究甲状旁腺激素对破骨细胞骨吸收功能的影响,以及成骨细胞和破骨细胞之间的相互作用。

Methods Osteogenetic culture medium containing different concentrations of atorvastatin 10^(-6, 10^(-7), 10^(-8)mol/L and control were added in the murine bone marrow cell subculture. The proliferation of the stromal cells was observed, and alkaline phosphatase staining and examination were done to check the differentiation ability of bone marrow cells. Alizarin red staining was performed to evaluate the mineralization of the osteoblasts in culture.

分别将10^(-8)、10^(-7)、10^(-6)mol/L的阿托伐他汀和空白药物加入传代培养的小鼠骨髓基质细胞,通过细胞形态学观察、细胞增殖率检测、碱性磷酸酶检测和钙结节染色,比较药物在成骨细胞分化过程中对细胞增殖和分化的影响。

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