细胞

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化，通过形态学观察，RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测，证实我们所建立的细胞分化模型是成功的，并观察到随着诱导时间的延长，miR-206在成肌分化过程中表达量逐渐增高的，表达改变具有阶段特异性。

Results Myoid/myofibroblastic differentiation occurred most commonly in fibrosarcomatous DFSP. It was recognized histologically as peripherally distributed or randomly scattered small eosinophilic nodules or short bundles, which were composed of bland spindle cells, closely resembling smooth muscle cells or myofibroblasts.

结果 肌样／肌纤维母细胞性分化多出现在纤维肉瘤型DFSP中，表现为肿瘤周边部或肿瘤内散在性分布的深嗜伊红色小结节或短条束，由梭形细胞组成，细胞多无异型性，核分裂象也罕见，形态上似平滑肌细胞或肌纤维母细胞。

Results ① Normal morphological structure of brain microvascular endothelial cells and neuroglial cells were injured by 1,2-dichloroethane, which damaged blood-brain barrier.

结果］染毒后的脑微血管内皮细胞呈进行性破坏，随着染毒剂量的加大细胞体积逐渐增大，胞膜模糊，细胞与细胞间、细胞与突触间连接减少，甚至消失；随着染毒时间的延长，在10、30、60min后可以观察到有进行性的细胞膜溶解破坏，胞核模糊，界限不清，突触变短。

Cells were collected to undergo HE staining and immunocytochemistry reaction against Thy-1 to identify RGCs. Axons length of retinal neurons and RGCs were measured by microruler under microscope respectively to evaluate the neurotropic effects of B vitamins in improving the regeneration and growth of axons for retinal neurons and RGCs. Finally, we observed the reaction of retinal neurons when exposed to the insult of glucose deprivation. The neuroprotection of B vitamins were also evaluated at the same time.

对B族维生素的神经营养和保护作用的研究过程分为三个阶段，首先确定维生素B族对视网膜神经元是否有神经营养作用，并根据细胞活力找出最佳有效作用浓度；然后在接种细胞的即刻用各种维生素的最佳有效作用浓度作用于视网膜神经元，并在不同时间终止培养，收集细胞，HE染色和RGCs的免疫细胞化学染色，分别测量视网膜神经元和RGCs的轴突长度，评价维生素B族是否有促进视网膜神经元和RGCs轴突再生伸长的神经营养作用；最后，观察视网膜神经元对低糖损伤的反应，及维生素B族对受损的细胞是否有保护作用。

Nither CDMP-1, nor cryopreservation can effect cell cycle of the chondrocytes.

结果：残耳软骨细胞在体外培养时随代数增加呈异染性的糖胺多糖减少，第1、3代Ⅱ型胶原阳性细胞比例约68％/～71％，第5代为23％、显著降低；冻存后第3代为65％，与同代数的细胞没有差别，100ng/ml浓度的CDMP-1可以将第4代细胞的阳性比例维持在66％，而且冻存和生长因子不会引起细胞周期的变化。

The proliferation stimulating effects on whole and nonadherent splenocytes of mice were assayed by MTT method; The cell cycles of whole splenocytes were tested with flow cytomytry.

通过MTT法测定小鼠全脾细胞或不粘附细胞的增殖活性，流式细胞仪检测小鼠全脾细胞的细胞周期。

METHODS: mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC，非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞，用自体乳腺癌细胞冻融抗原刺激DC，并和TDLNC共培养，以诱导肿瘤抗原特异性CTL。

METHODS: The mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC，非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞，用自体乳腺癌细胞冻融抗原刺激DC，并和TDLNC共培养，以诱导肿瘤抗原特异性CTL。

METHODS: Bone marrow mononuclear cells were isolated from 5M4/M5 leukemia patients with high CD14 expression, and then divided into 3groups: adherent leukemia cells, nonadherent blasts, and total unfractioned blasts.

取5例初诊CD14高表达的M4或M5型白血病患者的骨髓标本，分离单个核细胞(bone marrow mononuclearcells，BMMNCs)，将白血病细胞分为3组：贴壁白血病细胞组、非贴壁白血病细胞组及总白血病细胞组。

The lipid peroxidation of S. maxima cultured in the presence of 4 to 100mg Se/L analysed showed that the contents of free radicals by EPR and malondialdehyde increased, of which the latter was the products of lipid peroxidation. These resulted in the decrease of the contents of liposoluble matter and percentage of polyunsaturated fatty acids, and the increase of percentage of saturated fatty acids. Using dimethyl sulfoxide , which was oxidized by OH·to form the stable and nonradical compound, methane sulfinic acid , as a molecular probe, we found that the contents of MSA from S. maxima growing in the presence of selenium were higher than those without selenium added, i. e. selenium in vivo was able to scavenge OH. in this alga.

对4～100mg Se/L范围内培养的S.maxima进行脂质过氧化的分析结果表明，加硒条件下藻细胞EPR自由基的含量与脂质过氧化产物的含量增加，造成细胞脂溶性物质含量和不饱和脂肪酸百分含量下降，以及饱和脂肪酸的百分含量相对上升；使用DMSO作分子探针，用它与OH·的反应产物MSA来表示藻细胞OH·含量，发现加硒条件下藻细胞MSA含量低于未加硒的，即硒在藻细胞内具有清除OH·作用。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐