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The results indicated that there were only single mucosal mast cell sunset in rat mammary gland.They had various shapes.Most of mast cells were located in the interalveolar stroma and intralobular connective tissue.

结果 :AB- S染色显示,大鼠乳腺只存在黏膜型肥大细胞;无论是泌乳期还是静止期,乳腺肥大细胞大多分布于腺泡间和小叶间结缔组织中,细胞的形态各异,但细胞数量在静止期和泌乳期有显著差异,泌乳期的乳腺肥大细胞数量明显减少(P 。0 1)。

Some recent researches of abroad have found bone marrow cells can also differentiated into glomeruli cells in some conditions, differentiated into intercapillary cells, endothelium or podocyte in different animal models, but these reports are rare in the domestic.

国外的一些研究表明,骨髓细胞在一定条件下还可以向肾小球细胞分化,在不同的动物模型中分化成为肾小球的系膜细胞、内皮细胞和足细胞,但国内却鲜有报道。

The HBsAg was located in the intervillous space (21 placentas) and...

HBsAg主要分布于蜕膜血管内皮细胞(18例)、蜕膜细胞(21例)、滋养层细胞(8例)、绒毛间质细胞(8例)和绒毛血管内皮细胞(5例)的胞浆和绒毛间隙(21例)中。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant, which were called the wild tue strain group and the mutant stain group,respectively. The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.The induction of apoptosis was determined by flow cytometry.

将幽门螺杆菌野生株与cagA基因缺失突变株与BGC823细胞共培养,分别在6,12,24和48h观察细胞形态学变化,采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性,流式细胞仪检测细胞凋亡情况。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant,which were called the wild tue strain group and the mutant stain group,respectively.The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.The induction of apoptosis was determined by flow cytometry.

将幽门螺杆菌野生株和cagA基因缺失突变株和BGC823细胞共培养,分别在6,12,24和48h观察细胞形态学变化,采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性,流式细胞仪检测细胞凋亡情况。

Results Characteristic changes of HNE1 apoptosis were significantly observed after PDT, including early occurrence of cell condensation, karyopyknosis and karyorrhesis.

PDT 12h后,实验组HNE1细胞开始变形皱缩,细胞界限模糊,细胞透光度和立体感较差,治疗 2 4~ 4 8h后细胞发生凋亡。结论体外光动力学治疗对HNE1具有明显的杀伤效应并诱导HNE1细胞凋亡。

ResultsThe glandular cell in the endometrial implant after therapy with middle or high-dose of (10,15 gkg-1d-1) XiaoChaiHu Decoction showed characteristic features of apoptosis which displayed by the cell decreased in size, karyopyknosis , cytoplasmic and nuclear chromatin condensation , density increased and a lot of apoptotic bodies among cell. Whereas some stromal cell displayed degeneration and necrosis. The protein expression of Fas and Caspase-3 in endometriotic tissue of XiaoChaiHu Decoction group was higher than that in the endometrium.

结果中、高剂量小柴胡汤(10,15 gkg-1d-1)治疗后,异位内膜有较多腺上皮细胞出现凋亡的特征,表现为细胞体积变小,核固缩,胞浆和核染色质凝集,密度增高,细胞间凋亡小体,同时间质细胞中可见一些坏死细胞;异位内膜Fas蛋白、Caspase-3蛋白的表达水平明显高于其在位内膜。

After 4 days of serum deprivation, CPR-293 cells exhibited substantial nuclear condensation and fragmentation DNA laddering but HO-1/CPR-293 cells was not apparent. Thus HO-1 expression is sufficient to protect cells from serum deprivation-induced apoptosis. In the experiment, we used primary cultures of from HO-1 fibroblasts and their wild-type littermates fibroblasts CPR-293 cell and HO!

2生理加压诱导细胞凋亡我们用稳定的CPR细胞株和HO1和CPR在一起的细胞株实验,当浆液丢失4天后,CPR-293细胞表现出有实质的核浓缩和DNA梯状条带的形成,而HO1/CPR-293细胞则没有,显示HO1是充分保护细胞,避免在浆液丢失情况下而诱导的细胞凋亡。

RESULTS: hADSCs presented fibroblast-like morphological feature with a flocked array. The 3rd passage of hADSCs had unique immunophenotypes and they were positive for CD73, CD44, CD166, CD105 and CD29, but negative for CD31, CD34, CD45 and HLA-DR. Cell cycle result showed that they had the typical growth characteristics of stem cells, namely, 83.81% cells stayed at G0/G1 stage, only 16.19% cells were stayed at S+G2/M stage; The latent phase of the primary culture cells was 2 days prior to and after incubation, followed by 3-6 days of logarithmical proliferation, reached a peak at day 6, and entering the growth platform phase with lower growth speed.

结果:分离培养的人脂肪间充质干细胞为成纤维细胞样,呈漩涡状排列。P3代人脂肪间充质干细胞呈CD73,CD105,CD166,CD44及CD29阳性,而CD31,CD34,CD45及HLA-DR阴性;具有典型的干细胞增殖特点,83.81%细胞处于G0/G1期,仅16.19%细胞处于S+G2/M期;细胞在接种后前2 d处于生长潜伏期,第3~6天处于对数生长期,第6天达峰值,以后细胞生长速度减慢,进入生长平台期,平均五六天传代1次。

To sum up, both experiments in vivo and in vitro show that activating blood circulation to dissipate blood stasis has effects of inhibiting proliferation of malignant cell, inducing differentiations and impelling apoptosis. These perhaps were the machinisms of clinical effects. Considering the effects on DNA content, we dares inferred that YDQ inhibited proliferation by restraining the synthesis of DNA. It asks for our doing more work to research the underlying machinism with immunology, cytobiology, molecularbiology and so on.

总之,体内、体外实验均表明活血化瘀为主的疗法对恶性细胞有一定的增殖抑制、分化诱导和凋亡促进作用,这也许正是临床治疗MDS取效的原因所在,分析瘀毒清对细胞DNA含量的影响,我们推测认为瘀毒清可能通过抑制细胞DNA的合成进而抑制恶性细胞的增殖,其更深的机制尚待我们从免疫学、细胞和分子生物学等角度进一步研究揭示。

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