细胞

The germinative number of horsebeans and peas were raised by 44% with activated water by tourmaline. 200μl 1 X 10~6/ml mouse's H_(22) cell, 200μ 4X 10~6/ml splenic cell, 200μl 1 × 10~6/ml epidermal cell were all inhibited after the action of tourmaline. It is analyzed that the factors which effected the multiplication of the cells included direct action which are tourmaline's electric field, far-infrared, negative ions and solvent ions, and indirect effect which are small water clusters and pH value.

电气石活化水使蚕豆和绿豌豆种子发芽速度均提高44%。0.5g电气石分别对200μl 1×10~6/ml的小白鼠H_(22)肝癌细胞，对200μl 4×10~6/ml小白鼠脾细胞，对200μl 1×10~6/ml的小白鼠表皮细胞的增殖起抑制作用，分析认为，电气石影响细胞增殖的因素包括电场、红外线、负离子和溶出离子对细胞的直接作用和通过改变水分子结构、培养基pH值的间接作用。

After cocultured with reactive astrocytes, olfactory ensheathing cells can promote the proliferation of reactive astrocytes,and reduce the astrocyte gliosis and expression of inhibitory cytokines.

嗅鞘细胞作用于活化后的星形胶质细胞以后，能够促进星形胶质细胞的分裂增殖，同时能够减少星形胶质细胞胶质化，降低抑制性细胞因子的表达。

The dynamic changes of the number of T or B lymphocytes and the content of immunoglobulin G, IgM, IgA in peripheral blood, the number of CD〓, CD〓 lymphocyte subpopulation, T〓 cells and IgG, IgM, IgA antibody-producing cells, the ratios of CD〓 and CD〓 T cells in immune organs (thymus, bursa of Fabricius and spleen) and immune tissues (cecal tonsils and Harderian glands), the content of IgA, IgM, IgG in local fluid (tear, tracheal fluid, intestinal fluid and bile) of one-day chicks infected with chicken infectious anemia virus and CIAV-infected chicks immunized with Newcastle disease vaccine were determined respectively by immunohistochemistry, immunogold-silves and immunoenzyme staining in order to reveal mechanism of immunopathogenesis of chicks infected with CIAV and influence of immune responses of CIAV-infected chicks to ND vaccine.

中文题名雏鸡感染鸡传染性贫血病毒后免疫变化和对新城疫疫苗的应答及其免疫机理副题名外文题名 Immune changes of chicks infected with chicken infectious anemia virus and immune response and mechanism to Newcastle disease vaccine immunity 论文作者郑世民导师刘忠贵教授学科专业基础兽医学研究领域\研究方向学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数105页关键词鸡传染性贫血病雏鸡新城疫疫苗馆藏号BSLW /2003 /S858 /9 本项研究采用免疫组织化学、免疫胶体金及免疫酶方法，首次对CIAV感染1日龄雏鸡和CIAV感染雏鸡ND疫苗免疫及vNDV攻击后，其外周血液的T细胞、B细胞数量和IgG、IgM、IgA含量；中枢免疫器官胸腺和法氏囊，外周免疫器官脾脏及呼吸道和消化道局部粘膜免疫组织哈德尔腺和盲肠扁桃体的ANAE〓、CD4〓、CD〓T细胞、IgG、IgM、IgA抗体生成细胞数量和CD〓与CD〓T细胞比值以及泪液、气管液、肠液、胆汁的IgA、IgM、IgG含量的动态变化规律和CIAV感染对ND疫苗的免疫保护率等进行了全面系统地研究。

By using fluorescence microscopy of Hele cells and PC12 cells,probe Rh6GFe1 was shown to be capable of penetrating into living cells and imaging intracellular Fe~(3+)distribution.

活细胞Hele细胞和PC12细胞的荧光显微成像结果表明该探针能够穿透细胞并能识别细胞内的Fe~(3+)。

In this study, Mono-nuclear cells containing hematopoietic stem/progenitor cells were isolated from umbilical cord blood of normal full-term deliveries, healthy adult and heterocellular HPFH patient bone marrow by density gradient centrifugation. After simple purification and expansion in vitro, MNCs were induced to erythroid cell differentiation using different culture systems and cytokines including Epo, IL-3 and GM-CSF.

本研究首先利用密度梯度离心方法，从正常成人和异细胞型遗传性胎儿血红蛋白持续存在综合症家系患者的骨髓组织、健康足月正常分娩胎儿脐带血中分离得到了含有造血干／祖细胞的单个核细胞(Mono-Nuclear Cell，MNC)，进行初步纯化及体外扩增后，组合使用Epo、IL-3、GM-CSF等细胞因子，将造血干／祖细胞向红系方向进行诱导培养。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

Restrictive changes of TCR Vp repertoire and clonal expanded T cells could be found in peripheral blood T cells from patients with T-ALL. However, the clonal expanded T cells' property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. It may play a certain role on detection of minimal residual disease and design of anti-leukemia idiotypic vaccine. The dominant utilization of TCR VjJ repertoire could be found in peripheral blood T cells from patients with B-ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia-associated antigen. The clonal expansive tendency of T cells in V021 and VP23 subfamilies was rather obvious, which may be correlated with certain malignant B-cell clone. The CDR3 sequences of monoclonal expansive T cell in T cell strains were different, which was the base of developing DNA vaccine.

T-ALL患者外周血T细胞的TCR Vβ谱系出现限制性改变，均可检测到克隆性增殖T细胞，尚需进一步鉴定其性质(肿瘤性或抗原特异性增殖)，对于研究微小残留病变检测和设计抗白血病独特型疫苗均有一定的意义。B-ALL病人外周血T细胞的TCR Vβ谱系呈现优势利用的特点，并存在克隆性增殖的T细胞，这可能是机体对白血病相关抗原产生的特异性免疫反应；Vβ21和Vβ23亚家族T细胞发生克隆增殖改变的趋向性比较明显，可能与B-ALL相关抗原刺激有关。T细胞株中检测到的肿瘤克隆其CDR3序列具有高度的特异性，是构建DNA疫苗的基础。

Hormone level in maturation medium affected cumulus expansion of COC, but cumulus expansion became less dependent on hormone level with time of culture. Porcine immatured oocytes, granulosa cells from small antral follicles and cumulus cells of immatured oocytes secreted cumulus-expansion enabling factors. Granulosa cells from 3-6mm follicles did not produce CEEF, but there was some CEEF in the follicular fluid of this sized follicles.

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3—6mm卵泡的卵泡液中有CEEF。

Hormone level in maturation medium affected cumulus expansion of COC,but cumulusexpansion became less dependent on hormone level with time of culture.Porcine immaturedoocytes,granulosa cells from small antral follicles and cumulus cells of immatured oocytessecreted cumulus-expansion enabling factors.Granulosa cells from 3-6mm follicles did notproduce CEEF,but there was some CEEF in the follicular fluid of this sized follicles.Sun xingshen, Animal Histology and Embryology

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3-6mm卵泡的卵泡液中有CEEF。

This study demonstrated ①the infraglottic mucosa of the 〓 month human fetus iscandidate for culturing human laryngeal epithelial cells;②the cytokeratins of lowmolecular weight could be used as identified marker;③the basal and intermediatecells might possess the ability of bipotential differentiation and the ciliatedepithelium of the 〓 week fetal larynx might be a transitional form of thesquamous metaplasia.

抗低分子量细胞角蛋白抗体可用作细胞鉴定的标志；3.13周以后胎喉声门下粘膜为纤毛上皮，其基底细胞和中间细胞具有双向分化的潜能，在某些条件的刺激下，有可能化生为鳞状上皮细胞。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐