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In order to analyze experimentally the back-scattering micro-spectrum difference between human normal stomach epithetic cell and the cancerous cell to provide optical technique for clinical checking,a micro-spectral measurement setup for checking cell was established based on fiber confocal microscopically imaging technique combined with cell light scattering theory. It could provide micro-imaging and micro-medical spectral information simultaneously.

为了在实验上分析人正常胃上皮细胞与癌变细胞的显微后向散射光谱的区别,为临床医学检测提供实验依据,在光纤共聚焦显微成像技术基础上,结合细胞散射理论,建立了一套基于光纤共聚焦的细胞检测显微光谱分析装置,能够同时获取细胞显微图像和显微光谱医学信息。

The data shoewed that esculetin, resveratrol enhanced LPL expression up to about 1.2 ~3.5 fold compared with the control group. In addition to fibrates, however, no effect on LPL expression by curcumin was observed. We concluded that esculetin and resveratrol enhanced LPL expression on CHO-K1 cell. We hope that we can apply these drugs to mutant form LPL, furthermore apply theses drugs into clinical treatment.

利用药物处理有转染带有完整人类脂蛋白解脂酶启动子和脂蛋白解脂酶cDNA 序列(-1715 ~+1643)质体的CHO-K1细胞,从细胞培养液以及细胞溶解物中发现,除了curcumin之外所有药物都可以使得细胞培养液以及细胞溶解液内的脂蛋白解脂酶活性有1.2至3.5倍的上升,由此结果可知除了curcumin外,esculetin和白藜芦醇以及其他fibrate药物可降低三酸甘油脂血症。

Mucous membrane protective screen is formed by the join between cell of alvine mucous membrane and cell, atrophy of cell of alvine mucous membrane, wither dies, cellular connective can send alvine path to connect flabbily appear a gender to increase, provide passageway; chemical protective screen for the bacterium inside bowel and toxin the digestive juice that shows epithelial excretive mucus and alvine memory are in bowel and digest enzymatic the chemistry that waits for material to reach its to develop.

黏膜屏障由肠黏膜细胞细胞间的连接构成,肠黏膜细胞萎缩、凋亡、细胞连接的松弛可致肠道通透性增加,为肠内细菌及毒素提供通道;化学屏障指肠上皮细胞分泌的黏液及肠内存在的消化液和消化酶等物质及其发挥的化学功能。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞,建立小鼠T淋巴细胞活化、增殖的模型,以不同浓度的CPT作用于该模型,流式细胞术检测T细胞早期活化标志CD69分子的表达;以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数;以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂,以不同浓度的TMB-8及与环孢菌素A联合作用于该系统,用流式细胞术检测T细胞早期活化标志CD69分子的表达;以活体染料羧基荧光素乙酰乙酸染色流式细胞术,分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数;以碘化丙啶染色分析细胞周期的分布情况。

The proliferation and kinetics, cell cycle, clonalforming rate, and apoptosis of MEC- 1 cells, before and after FHIT gene transfection in vitro, and tumor loci formedon mice after injection of transferred MEC- 1 cells in vivo were observed by immunochemical staining, flow cytometryanalysis, and so on.

结果外源性FHIT基因转入黏液表皮样癌MEC- 1细胞后,S期细胞显著减少,G1期细胞增多;细胞倍增时间从(21.03±0.41)h延长为(26.86±0.71)h;软琼脂克隆形成率显著降低;细胞发生退行性改变和出现凋亡早期表现。

RESULTS:①At day 10, there were cells in clusters in the induction group, and inter-layer cells were round, outer-layer cells were long fusiform.

结果:①培养10 d时,诱导组细胞细胞团样改变,内层的细胞为圆形,外层的细胞呈长梭形并包绕着内层细胞

Gambogic acid suppresses the proliferation of Jurkat cells and Raji cells and arrested cell cycle of them at G0/G1 phase.

藤黄酸能抑制Jurkat细胞和Raji细胞增殖,使Jurkat细胞和Raji细胞细胞周期阻滞于G0/G1期。

Results (1) In the controls and the dilated intestine segment of the HD,CAD-positive ganglionic cells were observed in myenteric and submucosal plexus.

结果 (1)在正常对照组与HD扩张段组肠壁肌间和黏膜下层可见CAD只对神经丛中神经节细胞阳性表达,对神经纤维与神经胶质细胞均不表达。S-100染色与CAD染色相反,神经纤维与神经胶质细胞均阳性表达,神经节细胞阴性表达,表现为阳性神经丛中细胞状&空白区&。

Result: Moderate to strong immunostainning was observed at the circularmuscle,submucosal region in normal bowel and in ganglionic bowel from patients with HD.

电镜下在环形肌近粘膜缘及环形肌外层见肠间质细胞与平滑肌细胞之间、平滑肌细胞与平滑肌细胞之间有缝隙连接存在,在无神经节细胞肠段未见此结构。

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当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

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