细胞

In cuboidal cells the nuclear membrane sank downward dividing the nucleus into t wo or three segments and the perinuclear space and nuclear pores were wider than those in squamous cells.

另外 ，我们还观察到一种介于扁平形细胞和立方形细胞之间的中间型细胞，其胞质和核的发育明显好于扁平形细胞，但比立方形细胞差。

Pathology findings:there were 294 cases of clear renal cell carcinoma (83.76%),including 2 cases of cystis renal cell carcinoma ,27 cases of Grannular renal cell carcinoma ( 7.69 %), 14 cases of papilliform renal cell carcinoma , 6 cases of the mixed type renal cell carcinoma,10 cases of other cell types .

病理结果：透明细胞癌294 例(83.76%，其中囊性肾细胞癌2例)，颗粒细胞癌27 例(7.69%)，乳头状肾细胞癌14例(3.98%)，肾混合细胞癌6例(1.7%)，其它癌10例。

Pathology findings:there were 294 cases of clear renal cell carcinoma (83.76%),including 2 cases of cystis renal cell carcinoma ,27 cases of Grannular renal cell carcinoma ( 7.69 %), 14 cases of papilliform renal cell carcinoma , 6 cases of the mixed type renal cell carcinoma,10 cases of other cell types . Followup was achieved in 263 cases (74.93％),with tie 3、5year and 10 year survival rates being 70.45 %(124/ 176),53.3%(40/75) and 23.8%(5/21) respectively.

病理结果：透明细胞癌294 例(83.76%，其中囊性肾细胞癌2例)，颗粒细胞癌27 例(7.69%)，乳头状肾细胞癌14例(3.98%)，肾混合细胞癌6例(1.7%)，其它癌10例。263 例(74.93 %)获得随访，随访时间6个月～12 年，3 年、5 年和10年生存率分别为70。

Pathology findings:there were, 294 cases of clear renal cell carcinoma （83.76 %）, including 2 cases of cystis renal cell carcinoma , 27 cases of Granntdar renal cell carcinoma （ 7.69 %）, 14 cases of papilliform renal cell carcinoma , 6 cases of the mixed type renal cell carcinoma, 10 cases of other cell types .

病理结果：透明细胞癌294例（83.76%，其中囊性肾细胞癌2例），颗粒细胞癌27例（7.69%），乳头状肾细胞癌14例（3.98%），肾混合细胞癌6例（1.7%），其它癌10例。263例（74.93%）获得随访，随访时间6个月~12年，3年、5年和10年生存率分别为70.45%（124/176）、53.3%（40/75）和23.8%（5/21）。

Methods Totally 173 patients diagnosed as MDS according to FAB or WHO criteria with complete clinical and cytogenetical data were included in this reseach.

方法应用骨髓细胞短期培养法和染色体G显带技术，部分病例联合应用荧光原位杂交技术，对42例细胞遗传学检查具有+8异常克隆，16例具有-7/7q-异常克隆，以及55例虽然经常规细胞遗传学检查未检出异常克隆，但是骨髓涂片原始细胞≥0.10，按照FAB或WHO标准既往诊断为MDS的病例进行了临床及血液形态学和细胞遗传学的系列研究。

Methods Totally 173 patients diagnosed as MDS according to FAB or WHO criteria with complete clinical and cytogenetical data were included in this reseach. Among them 42 had +8 chromosome aberration, 16 had -7/7q-, and 55 had normal karyotypes and more than 0.10 blast cells in the bone marrow. Short term culture and G-banding techniques and in some specimens fluorescence in situ hybridization method were used to do chromosome analysis.

方法应用骨髓细胞短期培养法和染色体G显带技术，部分病例联合应用荧光原位杂交技术，对42例细胞遗传学检查具有+8异常克隆，16例具有-7/7q-异常克隆，以及55例虽然经常规细胞遗传学检查未检出异常克隆，但是骨髓涂片原始细胞≥0.10，按照FAB或WHO标准既往诊断为MDS的病例进行了临床及血液形态学和细胞遗传学的系列研究。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

It is well known that retinal pigment epithelial cells are predominant proliferative cells in PVR which is an excessively wound healing response occurring after RPE cell wounding. The activated RPE cells migrate from their normal, sessile state to vitreous and onto both surfaces of the retina, in which they begin to dedifferentiate, migrate, proliferate, change phenotype and secrete ECM.

既往研究证明视网膜色素上皮(retinal pigmentepithelial，RPE)细胞是参与PVR的主要细胞，PVR是视网膜脱离后RPE细胞损伤的一种自我修复过程，是一种过度的眼内创伤修复反应；RPE细胞在正常位置上处于静止状态，而在某些病理条件下，如视网膜裂孔形成和视网膜脱离后，RPE细胞脱离原位，开始去分化、移行、增生、发生表型转化并分泌胶原等ECM，最终在视网膜前后表面和玻璃体内形成具有收缩能力的增生膜，造成牵拉性视网膜脱离。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

EGF remarkably stimulated the dermal sheath fibroblasts, dermal Tibroblasts and demal papilla cells. Effection on the dermal sheath fibroblasts was the strongest. bFGF, GM-CSF, insulin and hydrocortisone had not effective on them.

EGF对真皮鞘细胞、真皮成纤维细胞和毛乳头细胞有显著的促生长作用，其中对真皮鞘细胞的作用最强，而bFGF、GM-CSF、胰岛素+氢化可的松对三种细胞没有明显的作用。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐