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To investigate the stantant changes of Ⅰ、Ⅱ、Ⅲ and PG expression of mandibular condylar chondrocytes stimulated by the different static compressive stresses, so that to make a primary understanding of the relationship between the differentiation and dedifferentiation of condylar chondrocytes and different compressive stresses; to investigate the stantant changes of the content of DNA of mandibular condylar chondrocytes stimulated by the different static compressive stresses, so that to make a primary understanding of the relationship between proliferentiation and apothesis of MCCs and different static compressive stresses.

观察单层培养的髁状突软骨细胞加载不同静压力后,Ⅰ、Ⅱ、Ⅲ型胶原以及蛋白多糖表达的变化,探讨不同静压力对髁状突软骨细胞的分化及去分化的即时效应;观察单层培养的髁状突软骨细胞加载不同静压力后,其增殖、凋亡及细胞周期的变化,探讨不同静压力对髁状突软骨细胞增殖及凋亡的即时效应。

Coculture of autogenic BMSCs with chondrocytes can promote the proliferation of chondrocytes and the production of chondral matrix. Cocultured cells for seeding can save a number of chondrocytes, shorten culture periods and reduce subculture times.

自体BMSCs与软骨细胞共培养,BMSCs能增强软骨细胞的增殖,促进软骨细胞基质合成,缩短软骨细胞培养时间和减少传代次数,可节省大量的软骨细胞,与DBM复合后能有效修复关节软骨缺损。

METHODS: Benzhydroxamic acid derivative E was artificially synthesized. The gastric cancer line 7901 was selected to serve as the target cell. The effect of medicine E on tumor cell activity was examined by cell culture technology in vitro. The change of morphology and the ultrastructure after treatment with medicine E was observed with the optic microscope and the electron microscope.

以胃癌细胞株7901为靶细胞,采用体外细胞培养技术评价苯异羟肟酸衍生物E对肿瘤细胞活性的影响,通过光学和电子显微镜对E药物作用后细胞形态学进行观察,用免疫组化和RT PCR方法检测细胞凋亡。

The quantitive value of DNA was 1.82% and 1.92% respectively in nonembryonic cells and the embryonic; during bicellular stage, quadracellular stage and multicellular stage, content of DNA increase slowly, and reach the peak at pear-shap ed embryonic stage keep high level at mature embryonic stage.

非胚性细胞与胚性细胞期的量化值分别为1.82%和1.91%;在二细胞胚、四细胞胚、多细胞胚时期DNA缓慢增长,随着胚性愈伤组织的发育,DNA的含量在梨形胚时期达到高峰;成熟胚的DNA含量虽有所下降,但仍维持较高水平。

The quantitive value of DNA was 1.82%and 1.92%respectively in nonembryonic cells and the embryonic; during bicellular stage, quadracellular stage and multicellular stage, content of DNA increase slowly, and reach the peak at pear-shaped embryonic stage keep high level at mature embryonic stage.

非胚性细胞与胚性细胞期的量化值分别为1.82%和1.91%;在二细胞胚、四细胞胚、多细胞胚时期DNA缓慢增长,随着胚性愈伤组织的发育,DNA的含量在梨形胚时期达到高峰;成熟胚的DNA含量虽有所下降,但仍维持较高水平。

The leaf epidermis structures of 6 species of Rhododendron had been observed under LM and SEM. The results show that all share together common features as follows: the leaf blades are typical back-abdomen bifacial types, the upper epidermis are formed by two or three layer bigger cells which inside layer cell is bigger than outside one, and the upper epidermis are covered with thicker cuticle.

利用光学显微镜和扫描电子显微镜观察了6种杜鹃属植物的叶片结构,结果表明,它们具有共同形态结构特征:典型的异面叶,上表皮由2~3层细胞组成,内大外小或内、外等大,有较厚的角质膜,无气孔器分布;下表皮均由1层细胞组成,排列紧密;栅栏组织由2层以上长柱形细胞组成,排列紧密;海绵组织细胞较短,排列较疏松,细胞间隙较大;均有表皮附属物。

Results Of 4 cases breast MALT-ML, 2 were CCL type, 1 CCL type with blastoid transformation, monotoid Bcell type. By immunohistochemistry, all cases were monoclonity of B-cell with follicular colonization and lymphoepithelia lesions.

结果:4例乳腺MALT-ML中2例为CCL细胞型,1例为CCL细胞型向母细胞样转化,另1例为单核样B细胞型。4例均显示B细胞单克隆性及滤泡克隆化和淋巴上皮病变。

The results showed that cMSCs and EA.Hy926 endothelial cells could attach and proliferate on scaffold, and the cell did not appear obvious abnormity in morphology. So it was indicated that the scaffold could not disturb the biological behavior of cMSCs and EA.Hy926 endothelial cells. It also showed that cells in dynamic culture were growing more bloomingly and metabolizing more rapidly than those in static culture.

两组研究结果发现细胞能在支架材料上粘附、增殖,并且细胞形貌正常,说明材料的加入不影响细胞的生物学行为,同时发现,动态培养的细胞比静态培养的细胞生长旺盛,代谢快。

The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 130dB infrasound treatment In 1 day group, Bcl-2mRNA and P53mRNA were expressed lightly in CA1-CA3 areas in hippocampus and dentate gyrus. Blood vessels dilated lightly. In 7d group, Bcl-2mRNA and P53mRNA were more expressed in areas mentioned above. Dilation of capillaries and small vessels was more obvious. Bcl-2mRNA and P53mRNA were strong positive in endothelial cells of vessels. In 14d group, Bcl-2mRNA and P53mRNA expression was obviously increased in pyramidal cells of hippocampus and granular cells of dentate gyrus, showing deep blue staining. a The endothelial cells in some vessels were also strong positive for Bcl-2mRNA and P53mRNA. Dilation of capillaries and small vessels was lessened. In 21d group, Bcl-2mRNA and P53mRNA expression was decreased in pyramidal cells. In 28d group, little change about the expression and distribution of Bcl-2mRNA and P53mRNA was observed. Lots of Bcl-2mRNA and P53mRNA were scattered in cortex of temporal lobe around hippocampus and callosal gyrus area. Dilation of capillaries and small vessels was lessened obviously. The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 90dB infrasound treatment.

次声作用后Bcl-2mRNA和P53mRNA在海马的表达,16Hz、130dB次声作用1d组:两者在海马〓区和齿状回轻度表达,海马内血管轻度扩张;7d组:两者表达在上述区域较1d增多,微血管和小血管扩张较显,血管内皮细胞内可见呈强阳性表达的Bcl-2mRNA和P53mRNA;14d组:海马锥体细胞和齿状回颗粒细胞内Bcl-2mRNA和P53mRNA阳性表达明显增多,呈深兰色,在部分血管内皮细胞内两者强阳性表达,微小血管扩张状态较7d减轻;21d组:锥体细胞胞浆内Bcl-2mRNA和P53mRNA表达减弱;28d组:上述表达及分布与21d组相比无明显改变,海马周围颞叶皮层和扣带回区域亦可见大量散在分布的Bcl-2mRNA和P53mRNA表达,血管扩张状态已明显减轻。90dB次声作用1d-7d后,较对照组无明显变化,14d-21d后轻度表达,28d后,Bcl-2mRNA和P53mRNA表达较强。

Methods Rat calvaria osteoblasts were cultured in the presence of 10 μg/mL-500 μg/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope.

将成骨细胞分别在添加了不同浓度(10μg/mL~500μg/mL)的nHA或nAg-HA/TiO2的培养基中培养2、6、8、24、72及120h,通过透射电镜观察、细胞计数及MTT法检测细胞超微结构、细胞增殖及线粒体活性,并计算单位细胞呼吸率。

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