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During the fertilization of angiosperms, the degenerated synergid induces a pollen tube to enter and break the pollen tube to release two sperm cells, which fuse with the egg and central cell.

在受精过程中,助细胞吸引花粉管向雌配子体生长,并接受花粉管长入细胞程序死亡助细胞中。接下来的花粉管停止生长和花粉管顶端破裂释放出2个精细胞的过程可能也依赖于助细胞

CaM mRNA was substantially expressed in thetapetum, stigma, pollen tube track, degenerated synergid and transfusion parenchymacells. Lesser but yet significant amounts of CaM mRNA were also localized in themicrospore mother cells, microspores, pollen, antipodal cells, egg cell and central cell.

钙调素mRNA在绒毡层、柱头、花粉管生长途径、退化助细胞以及维管薄壁细胞中大量表达,也可在小孢子母细胞、小孢子、花粉、反足细胞、卵细胞以及中央细胞中检测到。

Nonspecific cytotoxic cell is the evolutionary precursor of mammalian natural killer cell in teleostean fish, which was mainly separated from blood and lymphoid organ as the first barrier of defense to protect the animal against bacteria, virus and protozoa parasitism, it plays an important role in nonspecific immunity and also has a function of killing tumor cells in vitro.

自然杀伤细胞进化上的前体细胞在鱼类中被称作非特异性细胞毒性细胞,主要来源于血液和淋巴器官,是防御细菌、病毒、寄生性原生动物等入侵的第一道防线,在非特异性免疫中起重要作用,还具有体外杀伤肿瘤细胞的功能。

The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.

将幽门螺杆菌野生株与cagA基因缺失突变株与BGC823细胞共培养,分别在6,12,24和48h观察细胞形态学变化,采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性,流式细胞仪检测细胞凋亡情况。

The podocyte is a highly differentiated interdigitating cell,theist iniury is closely associated with the genesis and development of proteinuria,This article reviews manifestations,mechanism and the treatment of podocyte injury.

细胞是一种终末分化的多突状细胞,足细胞的损伤与蛋白尿的发生、发展密切相关,现通过对常见足细胞损伤的表现,及针对足细胞损伤的机制和治疗予以综述。

Transduction of TNF-α gene into TIL of human glioma by retroviral vectors and study of its biological activity in vitro. The development of retroviral vectors for TNF-α gene transfer and preparation of carrier cells-TIL have al- lowed transducing marked gene NeoR and purpose gene TNF-α into TIL cells respectively by use of monoclone tilter virus PLC-2 and PLJC-5. Detect the proliferation of TIL, expression of TNF-α and the anti-tumor activity of glio- ma TJ8510 cells in vitro.

在TNF-α逆转录病毒转移系统的建立及运载细胞TIL制备的基础上,利用构建的单克隆高滴度病毒细胞株PLC-2和PLJC-5将标记基因NcoR和目的基因TNF-α分别导入到TIL细胞中;对TNF-α基因转导后的TIL细胞进行细胞的增殖情况、TNF-α表达水平及对人脑胶质瘤TJ8510细胞的体外抗瘤活性的检测。

The CCND2 expression cell proliferation, cell cycle and cell apopotosis of the transfected LP-1 cells were studied by RT-PCR, trypanosome staining, flow cytometry and annexin V assay.

设计、合成CCND2短发夹RNA双链DNA模板,构建表达质粒pGenesil-2/CCND2,转染LP-1细胞,以RT—PCR检测对CCND2表达的抑制作用,以锥虫蓝染色计数和MTI检测细胞增殖,以Annexin V检测细胞凋亡,流式细胞术检测细胞周期。

To isolate mononuclear cells from foetus umbilic cord blood, After MNCs were incubated in the presence of APS group (APS 200 mg·L-1) and control group for 24 h,the cells were stimulated with Epo(5 IU/mL) for 0,2,5,30 min; STAT5 was measured by ICC and laser confocal scanning microscope, JAK2、STAT5 in nucleus and cytoplasm were measured by western-blotting-ECL in each group at different groups.

分离纯化胎儿脐带血单个核细胞,在常规体外培养体系加入APS(200 mg·L-1)作用细胞24 h,促红细胞生成素分别刺激0,2,5,30 min,免疫细胞化学与激光共聚焦显微镜观察细胞STAT5的表达;Western-blotting-增强化学发光法检测细胞JAK2与浆和核中STAT5的表达。

The observation showed that sterile plants and fertile plants in the pollen mother cell stage is not very different, when microspore mother cells of male sterile plants enter meiosis period, it could form normal tetrad cell , tetrad cell is normal in early development stage, In the uninucleate microspore stage , tapetal cells were vacuolization, the microspores were extruded to the middle by tapetal cells.

本研究利用石蜡切片法,结合光学显微镜技术对樱桃萝卜核质互作雄性不育系的不育株和对应保持系可育株的花药发育过程进行细胞形态学观察,观察结果表明:不育株与可育株在花粉母细胞时期差别不大,不育株的花药小孢子母细胞进入减数分裂期后,可以正常的形成四分体,四分体前期发育正常,而在单核小孢子期绒毡层细胞出现液泡化,绒毡层细胞向中间挤压小孢子。

METHODS: The MGC-803/ E2F-1 gastric cancer cells which were stably transfected with E2F-1, and containing unpossessed carriers, and the cells without transfection were performed MTT test to draw growing curve, and to compute cell proliferation rate. Cell cycles were examined by flow cytometry.

对稳定转染E2F-1的胃癌MGC-803/E2F-1细胞、转染空载体的MGC-803/EV及未转染的MGC-803细胞进行MTT法检测,绘制生长曲线和计算细胞增殖率;流式细胞仪检测各组细胞周期分布。

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