细胞

Methods: The Hela cells were cultured in vitro and treated with different concentrations(0.125～2.0 mg/ml) of matrine solution for 24～72 hours. MTT assay was performed to evaluate the cytotoxin effect of matrine on Hela cells. FCM was used to detect the apoptosis of Hela cells. Transmission Electron Microscope analyses were carried out to examine the cell morphology.

选用人宫颈癌Hela细胞进行体外培养，以0.125～2.0 mg/ml的苦参碱分别处理Hela细胞24～72 h后，MTT法检测细胞增殖，流式细胞仪检测细胞凋亡率，透射电镜进行形态学观察，RT-PCR半定量检测凋亡相关基因Bcl-2和Bax的mRNA表达水平。

Exogenous E2 alone has no effect on cell viability and apoptosis of H9C2 cells, but it can decrease the sensitivity of H9C2 cells to H_2O_2,hence,protect cells against apoptosis caused by oxidative stress.2Exogenous E_2 has downregulation effect on TβRⅠand TβRⅡof H9c2 cells,blocking ER may delete this effect of estradiol.H9c2 cells express ERβonly,suggests that E_2 may exert modulating effect on TGF-βpathway through ERβ.

外源性E2本身并不能影响H9c2细胞凋亡，但能够降低H9c2细胞对H_2O_2的敏感性，保护细胞抵抗氧化应激引起的凋亡。2外源性E2对H9c2细胞的TβRⅠ以及TβRⅡ具有下调作用，阻断ER可消除E2对TβRⅠ和TβRⅡ的下调作用；E2可进一步通过TGF-β通路降低受体后磷酸化smad2/3的水平；H9c2细胞仅表达ERβ，提示E2可能是通过ERβ发挥其对TGF-β通路的调控作用。

It is widely accepted that AP depolarize the plasma membrane and open voltage-dependent calcium channels , the calcium influx through VDCCs trigger the fusion of vesicles with plasma membrane and subsequent transmitter release.

现在被普遍接受的观点是当可兴奋细胞受到动作电位的刺激后，细胞的电压依赖性钙离子通道被打开，在动作电位的下降相引起细胞外钙离子的内流从而升高细胞内的游离钙离子浓度，触发许多细胞内的过程，譬如说神经递质分泌、信号转导和基因表达等。

Results hypso-purity (95%) astrocytes can be acquired and more depurative by passaging, part of astrocytes will be dying after nulli -nutrition;If applying methylprednisolone (10umol/l), cytoactive of damaged astrocytes will obviously super and expression of GFAP also obviously increase, all the thing is opposite for normal astrocytes.

结果 星形胶质细胞摇床后传至第三代，经鉴定其星形胶质细胞纯度达95%以上；缺氧3h后部分细胞形态变圆甚至死亡漂浮在正常细胞表面；正常组和损伤组分别加入MPSS3d后，损伤组细胞活性与GFAP表达均明显升高，但是正常则没有明显变化。

In the normal group, electron microscopy showed normal chondrocytes were oval, and cells and cell membrane were intact. In cytoplasm, there were abundant rough endoplasmic reticulum, dictyosome, and mitochondria with intact nucleus, and even chromatin. In the model group, chondrocytes exhibited obvious pyknosis and their shape was irregular. The areolae around cells disappeared; cell organs in cytoplasm coagulated to flaps which showed electron dense and was uneasy to observe; the shape of nucleus showed irregularity, and caryotin gathered densely.

电镜下正常对照组软骨细胞呈椭圆形，细胞及胞膜完整，包质内可见丰富的粗面内质网、高尔基体、线粒体，细胞核完整，染色质分布均匀；模型对照组软骨细胞明显固缩且外形不规则，细胞周晕消失，胞浆内细胞器凝成高电子密度的片状物不易分辨，细胞核不规则，染色质浓聚，散裂于核中。

Methods: Cells which belonged to the G1-phase were sorted by Multiparameter flow cytometry according DNA diploidy in asynchronous MOLT-4 cell lines, cells were synchronized at the G1-S boundary by thymidine, and western blot analysis and multiparameter analysis of DNA versus protein content was performed to examine cyclin A、B1、D〓、Eexpression in sorted G1-phase MOLT-4 cells and synchronized by double thymidine block in G1/S phase.

以MOLT-4细胞为模型，应用流式细胞术分选出非同步化生长的G1/S转换期起始处的G1期细胞，用"双thymidine阻滞法"将细胞同步在G1/S转换期起始处的G1期，采用DNA/cyclins双参数分析法及免疫印记法，检测同步化及非同步化G1期细胞中cyclinA、B1、D〓、E的表达。

It was a new mousegene in GenBank and named as SRG-L(spermatogenesis related gene expressed in the latestages of spermatogenic cells). The sequence of this cDNA has been deposited in GenBankwith the Accession No. AY352586. 2. Analyses of expression characteristics of SRG-L during mouse spermatogenesis.The results of RT-PCR and Northern Blotting indicated that no expression signal of thisgene could be observed in primitive type A spermatogonia and type B spermatogonia. Asignal first appeared in diplotene/pachytene spermatocytes, and was significantlyintensified in round spermatids and elongating spermatids, findly went down to beundetectable in mature spermatozoa.

RT-PCR和Northern Blotting分析表明：该基因在早期A型和B型精原细胞中不表达，从双线期初级精母细胞阶段开始表达并逐渐上调，在粗线期初级精母细胞、圆形精子细胞及长形精子细胞中均检测到高水平的mRNA，但在成熟精子中消失，具有明显的阶段特异性表达特征，并且组织特异性表达分析显示该基因在睾丸组织中的呈高表达。

BMSCs may reverse the disbalance of Th1/Th2 cells by regulating the cytokines production to take an important part in treatment of EAE.

在体外BMSCs通过化学诱导可以向少突胶质细胞分化；在体内BMSCs能向病变部位迁移，并部分分化成少突胶质细胞，同时BMSCs通过调节免疫系统的炎症细胞因子表达、CD4+和CD8+T细胞数和Th1和Th2细胞平衡，对EAE起治疗作用。

1 Day after injury ruptured capillary could be seen in ganglion cell layer, 4 weeks after injury cells in each layer arranged sparsely and disorderedly, in some RGCs chromatin became dense, 8 weeks after injury the cells in each layer became fewer and large amount of RGCs without nucleus could be seen.

3光镜下伤后1 d视网膜神经节细胞层出血，伤后4周视网膜各层细胞稀疏、排列欠整齐，GCL散在核染色质浓集、边聚的节细胞，伤后8周视网膜各层细胞明显减少，GCL内大量空化节细胞。

A double salt of platinous cyanide and another cyanide .Of, relating to, or producing a toxic effect on cells.

毒害细胞的，细胞毒素的毒害细胞的，有关细胞毒素的或造成对细胞毒害的

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。