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ResultsYAG laser could healing the pigment pathological changes, chromatophore present vacuolus.But the Vallate frame remaining Integrity.The surrounding tissue of chromatophore have not injury and still survival. Macrophage was heightened, pigment granule appear in the cell.

结果:YAG激光治疗色素病变可以消失,色素细胞呈现空泡状,但色素细胞轮廓的框架仍可完整保留,色素细胞周围组织并未受损伤仍可存活,并可见巨噬细胞增多,该细胞内出现色素颗粒。

Abnormality of cellular immunity existed in patient with chronical he...

慢性乙型肝炎患者细胞免疫功能紊乱,存在辅助性 T细胞减少及辅助性 T细胞细胞毒性 T细胞比例失衡等异常表现

A549 cells are more sensitive to CDDP-induced apoptosis(P.01) and less sensitive to THP andβ-Ele-induced apoptosis than A375p(P.01, P.05 respectively). Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic cells of A549 and A375p cell lines in all treated groups and necrocytosis were to be seen in some groups. Fluorescence imaging experiments demonstrated that accumulation of iASPP in cytoplasm but little distribution in nucleus in all groups. untreated groups in two cell lines presented a bright-green colour,followed by CDDP-treated groups,and THP-treated groups is the darkest one in all groups.

免疫荧光显示,未加药物处理的A549和A375p细胞胞质染色均匀呈亮绿色,核区较暗;CDDP及β-Ele处理组细胞为暗绿色,其中可见皱缩的凋亡细胞;THP处理的细胞胞质荧光弱呈灰绿色,由于THP嵌入DNA自发红色荧光与二抗的绿色荧光中和,胞核被染成桔红色,结果提示药物处理后A549和A375p细胞中iASPP的表达有不同程度的下降。

There was negative correlation between the ratio of 〓 cells and the course of disease of CA.

国内有些学者也先后对CA患者外周血T细胞亚群的变化进行了研究,结果表明:CA患者总T细胞无明显变化,〓细胞数降低,〓细胞数的增加,〓细胞比值降低。

Methods:Using cell culture technique,the inhibitory effects of NCTD on 3AO and AO cells were measured by way of crystal violet dyeing.The alterations of cell cycle induced by NCTD were analyzed with flow cytometry.

利用细胞培养技术,以结晶紫染色法测定NCTD对卵巢癌3AO和AO细胞的抑制作用,并利用流式细胞仪测定分析NCTD作用下两种肿瘤细胞细胞周期变化。

Results Osteoblasts from human bone marrow were successfully induced and differentiated; the cuttlebones were reshaped in cuttlebone hydroxyapatite for the first time; and some tissue engineering bones, such as the osteoblasts/...

结果 人骨髓细胞培养中诱导、分化出成骨细胞;首次将乌贼骨改性为乌贼骨羟基磷灰石;构建出成骨细胞/ CHA5 0 0 R、成骨细胞/乌贼骨羟基磷灰石、成骨细胞/聚羟基丁酸酯等组织工程骨。

The application of SH returns what can prevent the antioxidant inside body to eliminate, reduce cellular loss, proof SH is had fight oxidation [3] . 1 · the matrix of week of protective action cell of 3 pairs of cells can make the cell is avoided suffer a cytolysis poison...

SH的应用还可以阻止体内抗氧化剂的排除,减少细胞的损伤,证实SH具有抗氧化功能[3]。1·3对细胞的保护功能细胞周基质可使细胞免受具细胞溶解毒。。。

After cultured with tumor cytolysis antigen for 2 days, the cells were incubated with auto T cell separated by E-rosette formation. Methyl thiazolyl tetrazolium assay was employed to test inhibition rate of tumor specific cytotoxic T lymphocyte on HO9810 tumor cell line. Results Our results indicated that DC precursors in cord blood can be induced to differentiate and mature in the medium containing IL-4, GM-CSF, and TNF-α.

将获得的DC与用 E-花环分离脐血单个核细胞得到的T淋巴细胞共同培养3 d,然后分为两组,实验组2继续与不同比例的HO9810细胞共同培养3 d后获得细胞毒T淋巴细胞,对照组2除加入HO9810外,还加入了无关细胞,用四甲基偶氮唑蓝法检测其对HO9810细胞的抑制作用。

In autumn,there were GTH1 cells and GTH2 cells in GTH cells. A large number of secretory granules in GTH1 cells were circumnuclear; In GTH; cells cytoplast,there were two types of secretory granules,small circle and large circle, in small number.Structure of STH cells had no obvious changes.

秋季,在GTH细胞中发现GTH_1和GTH_2两种细胞,GTH_1细胞具有大量的分泌颗粒,并呈围核分布,GTH_2细胞胞质中具有数量较少的小圆形分泌颗粒和大圆形颗粒;STH细胞的结构无明显变化。

The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明: 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h,胞质突起率和去核率没有显著的差异,突起率可高达85.4%,去核率达到100%; 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h,对去核效果没有显著影响; 3 对于体外成熟18~23 h的卵母细胞,随着成熟时间的延长,盲吸法的去核率降低,但没有影响秋水仙素诱导胞质突起的比率和去核率; 4 两种去核方法对重构胚的发育没有产生显著影响,但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

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