细胞

Japonicus and analyzed the role of animal process of oocyte during ovulation and oocyte maturation. What's more, we studied the role of microtubules and microtubule organizing center upon GV migration. The oocyte of A. japonicus connects to the follicle via animal pole process. When placed in normal sea water, ovulation started spontaneously.

利用DTT诱导刺参卵母细胞成熟，综合运用光镜、电子显微镜及激光共聚焦技术观察卵母细胞成熟过程，分析了卵母细胞表面动物极突起在卵母细胞脱滤泡中的作用，以及卵母细胞动物极突起和微管组织在生发泡移动过程中所起的作用。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

ChinaObjective:To study the antitoxic role of vesicular monoamine transporter 2 (VMAT2) in transpgenic Chinese Hamster ovary cell.Methods:With the technology of transgene from PC12 to CHO,MTT reduction assay was used to detect MPP^+ toxic effect on wild type CHO and transgenic CHO.

利用转PC12细胞基因到CHO细胞中形成的转基因CHO细胞（cDNACHO，采用MTT比色法检测1-甲基-4-苯基吡啶离子（MPP^+对CHO细胞野生株（wtCHO和cDNACHO细胞的毒性作用，并观察利血平-VMAT2的特异性阻滞剂对MPP^+毒性作用的影响。

Sarcomeric actin expression was positive on day 10 after differentiation. the positive expression was in plasma. on day 6, plasma and organelle in cells increased, tight junction and intermediate junction were found and there was apophysis in cell surface and microfilament in apophysis.

电镜观察，未分化细胞为圆形或椭圆形，细胞间连接松散，细胞质较少，细胞器不发达；诱导后6 d，细胞间出现紧密连接和中间连接，细胞质增多，部分细胞表面出现突起，突起内可见微丝。

Skin suqamous cell carcinoma, a kind of skin tumor origined fromkeratinocyte or skin appendant organ suqmaous cell, is one of the most commonnon-melanoma skin cancers (about 20%).

咪喹莫特对人皮肤鳞状细胞癌SCL-1细胞株作用机制的研究前言皮肤鳞状细胞癌是起源于角质形成细胞或附属器鳞状上皮细胞的恶性肿瘤，是最常见的皮肤恶性肿瘤之一，其发病率约占全部非黑素性皮肤细胞癌的20％。

The two neck cells of the archegonium divided to fo rm four neck cells before fertilization, this division happened prior to the div ision of the central cell, but at times they divided synchronously.

颈卵器的2个颈细胞在受精前进行一次斜向分裂形成4个颈细胞，此分裂一般早于中央细胞的分裂，偶尔也可见颈细胞和中央细胞同时分裂。

Results: The numbers of Bombesin positive pulmonary cells, the lamina propria S-l00 protein, neuron-specific enolase positive nerve fibers, IgE positive cells, mast cells and IgE positive mast cells significantly increased in bronchiectasis. The changes of pulmonary endocrine cells, nerve fibers and IgE positive cells were more significantly in hyperplastic BALT areas. The S-100 and NSE were found in lymphoid tissue and BALT. A close contact was found between mast cells and the S-100 positive nerve fibers. An IgE positive outer zone was found on MC surface. Mast cells and IgE positive cells were seen in the bronchial epithelium and alveolar septa.

结果：支气管扩张症中，支气管上皮蛙皮素阳性细胞、固有膜S-100蛋白和神经特异性烯醇化酶阳性神经纤维、IgE阳性细胞、MC和IgE阳性MC均显著增多，且在支气管相关淋巴组织增生的区域上述肺内分泌细胞、神经纤维和IgE阳性细胞增多尤为显著，S-100蛋白和NSE阳性神经纤维分布於弥散淋巴组织和BALT中，MC与S-100蛋白阳性神经纤维紧密接触，MC表面有IgE阳性环状带，MC和IgE阳性细胞出现在支气管上皮间和肺泡壁。

In bicuspid and tricuspid valve, ECs were round or ovary, the ECs of lower surface of these valves were more apparent polarity than that of upper surface of the valve. The ECs appeared rectangle and arrange in regular, in pectinate muscles of left and right atrium, In inferior vena cava, ECs looked like round, ovary or irregular shape, without apparent polarity but with a few short microvilli.

在二尖瓣与三尖瓣，细胞多呈圆形或卵圆形，其瓣膜下面的细胞排列较上面的细胞极性明显；在左、右心房的梳状肌，细胞多呈长方形，排列较规则；在下腔静脉，细胞呈圆形，卵圆形或不规则形，极性不明显，有少量低矮的微绒毛。

Microspectrophotometer was used to measure the DNA content of 3 mucosa specimens of normal blad- der, 5 papillomas and 15 bladder transitional cell carcinomas. The transitional cell carcinoma was divided into 3 gradesand each grade group comprised 5 cases.

应用显微分光光度计检测膀耽正常粘膜、膀胱乳头状瘤及膀胱移行细胞癌共23倒(正常粘膜3例、乳头状瘤5例及移行细胞癌15例，移行细胞癌分三级，Ⅰ级、Ⅱ级、Ⅲ各5例)，其结果显示，正常粘膜上皮细胞和乳头状瘤细胞核及移行细胞癌细胞核Ⅰ、Ⅱ、Ⅲ各组之间胞核DNA含量均值有显著的差别(P.05～0.01)。

The antibodies were purified and the purity and specificity were identified by SDS-PAGE and Western-blot,respectively.Lung tissues from five pigs with PRRS were tested with the established method,which clearly demonstrated the abundant presence of viral antigens in the cytoplasm of alveolar macrophages,and to less extent in alveolar and bronchiole epithelial cells.

SDS-PAGE和Western-blotting检测结果表明，制得的兔抗PRRSV IgG纯度较高，具有良好的特异性，可用于原位检测病猪肺组织中PRRSV抗原的分布；Envision法的检测结果显示，PRRSV抗原的阳性表达产物主要出现在肺巨噬细胞胞浆内，其次为肺泡上皮细胞和细支气管黏膜上皮细胞；通过对5份经RT-PCR检测确认的PRRS病猪肺组织进行检测，均有较高的阳性细胞表达，平均阳性细胞率为46.5%。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。