细胞

Apoptosis, or programmed cell death, is a highly conserved, tightly controlledcell suicide process that is regulated by many different intracellular andextracellular events to ablate neoplastic cells in normal physiological functions.

细胞凋亡，又称程序性细胞死亡，是一个高度保守，受机体严格控制的细胞自杀过程，是在许多不同的细胞内外生理事件调控下的清除衰老和突变细胞的正常生理功能。

Results In those patients with marrow invasion , 4 cases in I phase（4.2%）,12 cases in II phase（12.6%）,36 cases in Ⅲ phase（37.9%）and 43 cases in Ⅳ phase(47.4%);small lymphocytic、diffuse cleaved cell、lymphoblastic lymphoma are main pathologic types;patients with mediastinal lymphadenectasis、splenomegaly and spleen violations are prone to catch bone marrow involvement;56 cases of anemia in patients with bone marrow involuement(58.9%), 42 patients with thrombocytopenia (44.2%),27 cases with leukopenia (28.4%),49 cases of patients leukocyte increased (51.6%),so,anemia is common;three were 30 cases with three items abnormal（31.6％）and 65 cases with one item abnormal at least (68.4%),the incidence of abnormal peripheral blood in leukocythemia patients were higher than that in patients with bone marrow infiltration,Particularly the patients with increased leukocyte or three items abnormalities were more common in leukemia; Abnormal cells in peripheral blood were observed in 66 cases (69.5%); chemotherapy efficiency reached 65.2% in the patients with bone marrow involuement,the median survival time was 11.5 months.

结果发生骨髓侵犯病例中I期4例（4.2%）， II期12例（12.6%），Ⅲ期36例（37.9%），Ⅳ期43例(47.4%)；病理类型以小淋巴细胞性，弥漫型裂细胞性（改为：弥漫性大B细胞型淋巴瘤）和淋巴母细胞性淋巴瘤多见；纵隔淋巴结肿大、脾脏肿大和脾受侵患者易发生骨髓侵犯；骨髓侵犯患者外周血中贫血56例(58.9%)，血小板减少42例(44.2%)，白细胞减少27例(28.4%)，白细胞增高49例(51.6%)，以贫血多见；三项均异常30例(31.6%)，至少一项不正常65例(68.4%)，淋巴瘤细胞白血病患者外周血象异常发生率高于骨髓浸润患者，尤其是白细胞增高或三项均异常者更常见于白血病；66例(69.5%)外周血分类中发现异常细胞；骨髓侵犯化疗有效率65.2%，中位生存期11.5个月。

Results In those patients with marrow invasion , 4 cases in I phase（4.2%）,12 cases in II phase（12.6%）,36 cases in Ⅲ phase（37.9%）and 43 cases in Ⅳ phase(47.4%);small lymphocytic、diffuse cleaved cell、lymphoblastic lymphoma are main pathologic types;patients with mediastinal lymphadenectasis、splenomegaly and spleen violations are prone to catch bone marrow involvement;56 cases of anemia in patients with bone marrow involuement(58.9%)恶性淋巴瘤症状, 42 patients with thrombocytopenia (44.2%),27 cases with leukopenia (28.4%),49 cases of patients leukocyte increased (51.6%),so,anemia is common;three were 30 cases with three items abnormal（31.6％）and 65 cases with one item abnormal at least (68.4%),the incidence of abnormal peripheral blood in leukocythemia patients were higher than that in patients with bone marrow infiltration,Particularly the patients with increased leukocyte or three items abnormalities were more common in leukemia; Abnormal cells in peripheral blood were observed in 66 cases (69.5%); chemotherapy efficiency reached 65.2% in the patients with bone marrow involuement,the median survival time was 11.5 months.

结果发生骨髓侵犯病例中I期4例（4.2%）， II期12例（12.6%），Ⅲ期36例（37.9%）恶性淋巴瘤分期，Ⅳ期43例(47.4%)；病理类型以小淋巴细胞性，弥漫型裂细胞性（改为：弥漫性大B细胞型淋巴瘤）和淋巴母细胞性淋巴瘤多见；纵隔淋巴结肿大、脾脏肿大和脾受侵患者易发生骨髓侵犯；骨髓侵犯患者外周血中贫血56例(58.9%)，血小板减少42例(44.2%)，白细胞减少27例(28.4%)，白细胞增高49例(51.6%)，以贫血多见；三项均异常30例(31.6%)，至少一项不正常65例(68.4%)，淋巴瘤细胞白血病患者外周血象异常发生率高于骨髓浸润患者，尤其是白细胞增高或三项均异常者更常见于白血病；66例(69.5%)外周血分类中发现异常细胞；骨髓侵犯化疗有效率65.2%，中位生存期11.5个月。

Our results demonstrate: The structures of the organs are normal, and the shapes of cells are clearly visible. There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum. Three bands with a molecular mass close to 120KDa、110KD and 98KDa were identified by Western Blot. The Ob-R levels of 120KDa in abomasum were significantly higher than that of in small intestine. The levels of 110KDa were similar in the two organs. The expression of 98KDa Ob-R was weak.

HE染色结果显示，各组织结构正常，细胞形态清晰可见；免疫组织化学SABC染色显示，在皱胃胃体部固有层胃底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒；western Blot 实验发现，在胃和小肠均检测到120KDa、110KDa和98KDa三条带。120KDa长型瘦素受体蛋白在胃中表达量显著高于小肠中的表达；110KDa的短型瘦素受体蛋白，在小肠和皱胃中表达量接近。98KDa短型受体蛋白在胃和小肠表达均较弱。

Results:The results show that the survival rate of mammary epithelial cells were decreasing gradually with the increasing of the time of TGF-β1 treatment, and the survival rates of 24 h and 48 h groups were significantly lower than that of control group (p.05); the LDH activity of 6 h、12 h、24 h and 48 h groups were significantly higher than that of control group (p.05); the DNA degradation arisesed in 12 h, 24 h, and 48 h groups' mammary epithelial cells; the activity of Caspase-3 reach to a peak at 24 h, which was significantly higher than that of control group (p.05); the acinus of epithelial cells appears to break off and collapse with the increasing of treatment time, but the leydig structure keeps integrate;...

结果表明：1、5、10 ng/mL的TGF-β1均能抑制乳腺上皮细胞的增殖，且随浓度增加抑制作用加大，呈现剂量依赖性，其中10 ng/mL TGF-β1组与对照组差异显著（P.05）；10 ng/mL TGF-β1作用于乳腺上皮细胞24 h，随作用时间延长细胞凋亡率逐渐增加，6 h、12 h和24 h组与对照组相比差异极显著（P.01）；LDH活力也不断升高，6 h、12 h和24 h组与对照组差异显著（P.05）；DNA随 TGF-β1作用时间的延长发生不同程度降解；TGF-β1作用2 h~6 h细胞大量表达HSP70，6 h达到高峰，而后下降，2~12 h的表达量均显著高于对照组（P.05）。结论：TGF-β1可以抑制奶牛乳腺上皮细胞的增殖，具有浓度依赖性。10 ng/mLTGF-β1。。。

Results AQP1 is expressed at the apical and basolateral membrane of the microvascular endothelium; AQP3 was detected at basal cells of both the bronchiole epithelium and submucosal gland acinus; AQP4 is present in the basolateral membrane of columnar cells in bronchiole; while AQP5 is expressed in the apical membrane of type Ⅰ pneumocytes, and also at the apical of columnar cells of superficial epithelium and submucosal gland acinar cells.

结果本研究发现AQPs基因在羊肺中的表达分布与人相似，AQP1在肺内的毛细血管内皮细胞表达；AQP3在小支气管黏膜上皮的基底细胞的基侧膜表达，AQP4存在于小支气管黏膜上皮的柱状纤毛细胞的基侧膜；AQP5存在于Ⅰ型肺泡上皮细胞的顶质膜，存在于小支气管黏膜上皮柱状纤毛细胞，以及在气道黏膜下腺的腺细胞的顶质膜表达。

K562 cell cultivated in suspension and adhesively cultivated with MSC were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of...

对悬浮培养和与MSC黏附培养的K562细胞绘制细胞生长曲线；用流式细胞术测定细胞周期并观察化学药物对细胞存活率和凋亡的影响；用RT-PCR技术检测MDR1基因表达。

This hyperacetylation causes cell cycle arrest and cell death in cancer cells but not in normal cells.

这种高度乙酰化造成癌症细胞的细胞周期阻断和细胞死亡，而不造成正常细胞的细胞周期阻断和细胞死亡。

There are some little cells with diameter 4.5~8.3 μm in molecular layer. Purkinje cell is like a pear in Purkinje cell layer, it's base width is 17.8~25.6 μm, it's height is 18-30 μm. There are lots of rough endoplasmic reticulum, free ribosome, mitochondria, golgi body, lysosome, etc. in Purkinje cell. Granular cell ratio of nucleus to cytoplasm is high in granular layer.

分子层含少量小细胞，细胞大小在4.5~8.3μm； Purkinje细胞层细胞体呈梨状，底宽在17.8~25.6μm之间，高约为18~30μm，细胞中含有丰富的粗面内质网、游离核糖体、线粒体、高尔基体、溶酶体等；颗粒层的颗粒细胞细胞核大，细胞质少。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果：①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后，逐渐呈灰黄色，表面粗糙，出现裂隙；当压力逐渐减小后，变黄、粗糙、有裂隙的软骨颜色逐渐恢复，变得有光泽②光镜下可见表面光滑、平整，细胞分布均匀、整齐，层次清楚的软骨在关节面压力增大后，表面变粗糙、缺损，细胞排列紊乱、染色不均；当关节面压力逐渐减小后，软骨表面逐渐修复，细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达，术后3周内表达逐渐变弱，从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后，软骨细胞逐渐变扁，胞质中内质网膜、高尔基体减少，胶原排列紊乱；当关节面压力减小，软骨细胞形态逐渐恢复正常，胞质中内质网膜、高尔基体数量逐渐恢复；胶原纤维排列逐渐有规则。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。