细胞

The inhibition of SPG-Rg3 on the growth of MCF-7 cells was detected by MTT assay,IC50 was calculated to obtain the effective concentration; flow cytometry was used to observe the cell cycle of MCF-7 cells; AO/EB fluorescence double-dye technology was performed to observe the apoptosis of cells in morphology,immunocellularchemistry and RT-PCR were utilized to investigate the apoptosis of MCF-7 cells and its relationship with caspase-8 from protein level and molecule level.

采用MTT法观察SPG-Rg3对MCF-7细胞生长的抑制作用，并计算出IC50，依据IC50值确定SPG-Rg3的有效浓度；流式细胞术检测SPG-Rg3作用后MCF-7细胞周期的变化；AO/EB双染从形态学上观察SPG-Rg3对MCF-7细胞凋亡的作用；免疫细胞化学染色和RT-PCR技术分别从蛋白水平和分子水平上检测SPG-Rg3对MCF-7细胞的诱导凋亡作用及其与caspase-8基因的关系。

Cerebral cortexes were removed and then dissociated, measured with flow cytometer analyze cell apoptosis, the morphological changes were examined by electron microscope.(2) Measured cell apoptosis of cerebral cortexes with TUNEL.(3) detected expressions of Bcl-2, Bax and Caspase-3 proteins with the technique of immunohistochemistry.

1取大脑皮层细胞制成细胞悬液，流式细胞仪分析细胞凋亡改变，电镜观察细胞超微结构的变化；(2)用原位缺口末端标记法检测凋亡细胞百分率；(3)免疫组化法检测Bcl-2、Bax和Caspase-3蛋白表达。

Methods: After LNCaP and PC3 cell lines were affected by 10, 25, 50, 75, 100 μmol/L curcumine respectively, the cell activity was assayed with MTT method at 5, 12 and 24 hours respectively; Electronic microscopy and flow cytometry were adopted to observe the morphological changes and the cell cycle of LNCaP and PC3 cell lines at 24 hours.

分别用10、25、50、75、100μmol／L浓度的姜黄素对LNCaP及PC3前列腺癌细胞株进行体外干预，5、12、24 h后四氮甲唑蓝比色法检测细胞生长活性；24 h后透射电镜观察细胞超微结构变化，流式细胞仪测定细胞凋亡及细胞周期的变化；5 h后Western印迹法检测细胞内IκBa的表达。

Methods Human monocyte cell line THP-1 was cultured and induced to macrophage by PMA treatment. The effect of 9-cisRA on the differentiation induced by PMA was studied. Cell morphology was observed by phase contrast microscope and the adhesion rate of THP-1 was detected by methyl thiazolyltetrazolium assay. The cell surface markers involved in differentiation of mono cyte/macmphage (CD11b, CD36) were analyzed by flow cytometry.

体外培养人单核细胞系THP-1，经佛波酯诱导分化为巨噬细胞，同时给予9-顺式维甲酸对佛波酯诱导分化进行干预，通过相差显微镜观察细胞形态，MTT比色法检测细胞贴壁率，流式细胞仪检测与单核／巨噬细胞分化有关的细胞表面标志物CD11b和CD36的表达。

It is further confirmed by the experiment of muti-inducement that MSMPs can differentiate into osteocyte sand fibrocartilage cytes and it is osteo-chondro progenitor cells with the potential ability to evolve muti-differentiated. This added evidence to the view that condylar cartilage differentiated from osteo-chondro progenitor cells in the mesenchymal cells of mancibular periosteal.

体外分离培养鼠胚下颌体骨膜间质细胞并研究其生物学特性，通过多向诱导实验进一步证实：下颌骨膜间质细胞可能为骨—软骨的前体细胞，可分化为成骨细胞及纤维软骨细胞，具有多向分化潜能，此结果进一步支持髁突软骨是由下颌骨膜间质中骨—软骨前体细胞发育而来。

Results: Compared with control group, apoptosis cells increased from 0.5%to 10%(some even to 15%) after 24,48 and 72 h action of -300,-500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48-72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound-vesicles.

结果：-300、-500和-1 000 V驻极体作用成纤维细胞24、48和72 h以后，与对照组相比，成纤维细胞的凋亡量从0.5％增至10％（部分可达15％）；驻极体作用成纤维细胞48～72 h，出现细胞凋亡特有的形态学特征，即：细胞异染色质边集，细胞裂解，可见凋亡小体。

Objective To investigate the dose-effect effects of th ree relating bone growth factors, dexamethasone, recombinant human basic fibro blastic growth factor and recombinant human bone morphogenetic protein- 2 (rhBMP-2), on proliferation and differentiation of periosteal cells so as to provide experimental basis for their further application in bone tissue engineer ing.

骨膜细胞是骨修复、重建过程中重要的功能细胞，在一定条件下具备向成骨细胞分化，分泌骨细胞外基质的潜能，因此成为骨组织工程研究中构建细胞-材料复合体非常重要的种子细胞。

Methods: The CD34+ cells and AC133+ cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34+ and AC133+ selection cells was observed and the clone formation of the CD34+ and AC133+ cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (in7d, 14d, 21d.). The rate of cell apoptosis was determined.

常规富集人骨髓CD34+及AC133+细胞，应用本研究组设计并已经证实的细胞因子组合方式，对人骨髓CD34+及AC133+富集细胞进行体外对照培养，分别观察CD34+和AC133+富集细胞的扩增情况；应用甲基纤维素半固体培养法，观察不同组别培养7、14、21d CD34+和AC133+富集细胞的集落形成情况及细胞凋亡率。

Methods CD34(superscript +) cells and AC133(superscript +) cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34(superscript +) and AC133(superscript +) selection cells was observed and the clone formation of the CD34(superscript +) and AC133(superscript +) cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (during 7d, 14d, 21d.). The rate of cell apoptosis was determined.

常规富集人骨髓CD34及AC133细胞，应用本研究组设计并已经证实的细胞因子组合方式，对人骨髓CD34及AC133富集细胞进行体外对照培养，分别观察CD34和AC133富集细胞的扩增情况；应用甲基纤维素半固体培养法，观察不同组别培养7、14、21d CD34和AC133富集细胞的集落形成情况及细胞凋亡率。

The uptake of 〓I-OxLDL in adipocytes was determined by gamma ray counter and normalized to protein concentration of each sample, was expressed as nanograms of 〓I-OxLDL uptake per milligram of cell protein. Reverse transcription polymerase chain reaction was used to evaluate PPARγ and CD36 mRNA expressions.

采用酶联免疫吸附法检测血浆IL-6以及脂肪细胞分泌IL-6的水平；采用gamma射线计数仪来检测脂肪细胞对〓I-OxLDL的特异性摄取情况，采用改良Lowry法进行细胞蛋白浓度测定，细胞对〓I-OxLDL的特异性摄取量以ng／mg细胞蛋白表示；采用逆转录聚合酶链式反应来检测PPAR γ和CD36 mRNA在脂肪细胞的表达情况。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。