细胞

In experiment 4, Schwann cells were seeded on PDLLA-T3 with the density of 2×106cells/ml, the foams were sterilized by infrared and removed air from the pore to facilitate the infiltration of cell suspension.

实验三，观察材料对培养雪旺细胞的影响，在雪旺细胞培养皿内加入小块甲状腺激素人工神经材料，发现该材料能使雪旺细胞数增加，刺激雪旺细胞分泌更多的NGF，提高雪旺细胞的活性，提示该材料对雪旺细胞有正性促进作用。

Results: The growth of NB4 cells was inhibited significantly after treated with berbamine at different concentrations for different time points, the IC50 value was 3.860μg/ml at 48 hours. Morphology analysis showed the characteristics of apoptosis, and the DNA agarose electrophoresis showed the typical DNA ladder. The apoptosis rate increased from 2.83% to 58.44% after treated with berbamine at 12μg/ml for 18 hours. The expression of PML-RARα mRNA presented no significant changes, however, Survivin mRNA was decreased dramatically.

结果：经不同浓度小檗胺处理不同时间后，NB4细胞生长出现明显的抑制，并呈现明显的时间剂量依赖性，48h IC50为3.860μg/ml；细胞形态学观察可见细胞凋亡现象，DNA琼脂糖电泳显示典型的凋亡梯形图：流式细胞仪检测发现细胞凋亡率明显增加，经48h作用后，凋亡率从2.83%增至12μg/ml时的58.44%；虽未发现药物作用后PML/RARα基因表达量的改变，但检测到NB4细胞经药物作用后Survivin基因表达量的减少和Caspase3表达的增加，Caspse3阳性率从对照组的2.06%增至12μg/ml时的70.89%。

Cell proliferation was measured with cell number,cell metabolic activity (WST-1 cleavage),DNA synthesis and cell cycle assayed with flow cytometry.

进行新西兰白兔心脏心肌成纤维细胞的培养；测定细胞计数，细胞活性（WST－1分解）和DNA合成，并以流式细胞仪测定细胞周期等以表示细胞增殖。

Methods: Cardiac myofibroblasts derived from heart of New Zealand rabbit were cultured and identified. Cell proliferation was measured with cell number,cell metabolic activity (WST-1 cleavage),DNA synthesis and cell cycle assayed with flow cytometry.

进行新西兰白兔心脏心肌成纤维细胞的培养；测定细胞计数，细胞活性（WST－1分解）和DNA合成，并以流式细胞仪测定细胞周期等以表示细胞增殖。

Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明，AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化；TUNEL检测呈阳性，说明有染色体DNA出现断裂；Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露；电镜观察的结果显示，转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

On the basis of comparative analysis of the relevant micro-imagemosaicing algorithms, a suitable algorithm is choosed to combine local images to ahigh-resolution images of greater vision, which is better for pathologist to observe cervical cellmorphology. By some measurement algorithms of pathological cells and metal particulate which are reported in magazines, cervical cell parameters measuring and nucleus plasm ratio analysisare better realized.

本论文通过将成熟的图像处理算法有针对性地用于宫颈细胞图像处理，较好的实现了对宫颈细胞图像几何变换，色彩变换，平滑，增强，边缘检测；在对比分析相关显微图像拼接算法的基础上，选择合适算法将局部图像合成更大视野的高分辨率图像，供医生更好的观察细胞形态；将相关文献中报导的处理病理细胞和金属微粒形态测量的算法用于宫颈细胞形态参数的测量，较好的实现了细胞形态参数测量及细胞核浆比分析等功能。

Human hair keratin ; spinal cord injury ; rat ; tissue engineering ; tissue-engineered spinal cord ; connector ; morphology ; praxiology ; neuron ; axis-cylinder ; nerve fiber ; myelin sheath ; astrocyte ; oligodendrocyte ; microglia ; ependymal cell ; neural stem cells ; macrophage ; multinuclear giant cell ; lysosomal enzyme ; ubiquitin system ; suppression subtractive hybridization ; gene express ; CytohromeoxidaseⅢ; amino acid transporter system A2（Ata2）; Peroxiredoxins 5（prd 5）; cysteine proteinase inhibitor cystatin c

国家自然科学基金；广东省自然科学基金人发角蛋白；脊髓损伤；大鼠；组织工程学；组织工程化脊髓；桥接物；形态学；行为学；神经元；轴突；神经纤维；髓鞘；星形胶质细胞；少突胶质细胞；小胶质细胞；室管膜细胞；神经干细胞；巨噬细胞；多核巨细胞；溶酶体酶；泛素肽酶系；抑制消减杂交；基因表达；细胞色素氧化酶Ⅲ；氨基酸转运蛋白系统A2；抗过氧化物蛋白5；半胱氨酸蛋白酶抑制剂

The adherent cells were mounted on a cover slip and stained with Hoechst 33342,and subsequently stained with mouse anti-human ant...

结果荧光显微镜下可见BGC-823细胞系中存在SP细胞，且该类细胞体积较小，约占细胞总数的1.8%；免疫荧光染色可见爬片细胞中ABCG2+细胞即为SP表型细胞。

In order to overcome some of the disadvantages of plant cell suspension culture, such as variability and shear sensitivity of plant cells, limited transfer of oxygen to the culture, mixing and relative low metabolites production, etc.

本文针对悬浮细胞培养中植物细胞剪切敏感性、溶氧及混合困难和次生代谢产物合成能力低等问题，进行固定化长春花植物细胞培养生产生物碱过程的研究，主要涉及固定化方法、影响固定化长春花植物细胞合成生物碱能力因素、及有关固定化长春花植物细胞反应器等问题，应用模糊集理论探讨固定化细胞培养过程的基本规律。

RESULTS: In the retinal region without light, the structure of every layer was clear, cells in neuroepithelial layer arrayed in rule, some bubble presented in external granular layer and internal granular layer, RPE cells were compact, and the color of pigment article was coincident. In the region with direct blue light and that with 30J/cm2+Acrysof one-piece/ pMMlA, cells on photoreceptor and external granular layer were lost partially, bubble increased, RPE cells were with different sizes, and cell edema, cell lost and pigment article cluster could be seen.. In region with 30J/cm2+Natural, a little disorganization could be seen comparing to that without light, but more normal than those with Acrysof and direct eradia tion.

结果：未照射部位：视网膜各层次结构清晰，神经上皮层内各细胞排列规则，整齐，RPE细胞排列紧密均匀，色素颜色一致；单独光照和透过Acrysof一片式I0L：光感受器层部分细胞丢失，外核层可见固缩核，浓密，内颗粒层细胞大量丢失，内丛状层内空泡明显增多，RPE表面，细胞大小不均，可见肿胀、缺失，细胞内色素浓密、聚集成块；透过Nat-ural一片：与未照射处相比，视网膜各层次结构仍有部分破坏，但程度明显减轻。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。