细胞

Under inverted microscope, stereognosis of neurons died down, there were blank bulb in the neurons, dark particle were seen in the neuritis; the result of MTT test showed that the survival rate of neurons was obviously decreased. Res 40μmol/L increased the survival rate of neurons. Aβ(subscript 25~35) dose-dependently increased the level of MDA, decreased the level of GSH. Res dose-dependently increased the level of GSH, decreased the level of MDA.

结果：Aβ(下标 25~35)在终浓度为2μmol/L时神经元形态有明显的变化，细胞立体感减弱，胞体内空泡形成，细胞突起内可见黑色颗粒状物；细胞的存活能力明显下降，与对照组相比较有显著性差异(P.05)；Res在终浓度40μmol/L时，能明显提高细胞的存活能力。Aβ(下标 25~35)可以明显升高细胞培养上清液中MDA含量，降低GSH的含量；Res可使细胞培养上清液中MDA含量下降，GSH含量增加。

Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.

构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体，将其导入逆转录病毒包装细胞PA317，通过G418抗性筛选，得到携带目的基因的包装细胞并鉴定；应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞，观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响；建立C6胶质瘤大鼠脑内移植动物模型，将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中，观察其治疗作用，并初步探讨其机制。

The prolif- erative ability of CD133 + tumor cells in vitro was estimated by MTT assay, and the proliferative ability of CD133 +, CD133 - and control SUNE cells were statistically compared. Finally, the immunocytochemical staining method and flow cytometry were used to detect differen- tial ability of CD133 + tumor cells in vitro .

采用免疫荧光细胞化学技术及流式细胞仪检测鼻咽癌细胞株SUNE中CD133的表达情况以及CD133+细胞体外分化能力；免疫磁珠分选技术纯化CD133+肿瘤细胞；MTT法检测CD133+细胞的体外增殖能力，并将其与CD133-及未分选细胞进行比较。

The result is consistent with previous studies. Whereas, tapetal cells of sterile anthers were disintegrated since tetrad cells were disjoined, indicating that disintegration of tapetal cells is earlier occurred in sterile anther than that in fertile anther. DNA ladder revealed that the cleavage of nuclear DNA into oligonucleosome-sized fragments to

结果表明，在花粉母细胞减数分裂后的四分体解离时期，不育花药的绒粘层细胞较可育花药提前开始裂解；在不育系S-Mo17rf3rf3花药和花粉S-(rf3)中均明显出现PCD过程的DNA片段化以及线粒体细胞色素C外渗的现象，证明了玉米CMS-S的花粉败育与花药绒粘层细胞的提前凋亡和小孢子细胞的细胞程序性死亡有关。

Results: Cords could be found both in the left and right ventricles. The frequency of the ventricular cords in the left and right ventricles was respectively 88.89% and 85.19%. The false tendon were classified into four types: muscular, conductive, mixing and tendinous. The muscular cords were composed of cardiomyocytes. The conductive cords contained Purkinje cells, T cells and a few P cells. The mixing cords consisted of cardiomyocytes and a few P cells.

结果：左、右心室均存在心室条索，出现率分别为88.89%和85.19%；心室条索分为肌型、传导型、混合型和腱型4种类型，肌型由普通心肌细胞构成；传导型含有Purkinje细胞、移行细胞和少量的P细胞；混合型含心肌细胞和少量P细胞；腱型由结缔组织构成。

In order to study the mechanism of the inhibitory effect of salvia miltiorrhiza and tetramethyl pyrazine on scar fibroblast, the DNA content of fibroblast and the all distribution in cellular cycle was measured by FCM. The hypertrophic scar tissue of chest was chosen for primary culture of fibroblast.

摘 要 应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化，发现药物在一定浓度作用下，DNA指数无明显变化；丹参使C2-M期细胞分布增多，G2-M期延长；川芎嗪使G2-M期细胞分布增多，G2-M期、S期延长；药物使细胞群体倍增时间延长，与浓度呈明显直线正相关。

Result and contrast group quite, active of CD4+T/CD8+T value and cell of the CD4+T before observing group method, NK cell is reduced apparently, and CD8+T cell is elevatory, after concerning; technique in installment with TNM 2 weeks relatively active of CD4+T/CD8+T value and cell of the CD4+T before art, NK cell is apparently elevatory, and the 4 A after CD8+T cell reduces; art is followed visit discovery, after afore-mentioned index change trends are the same as art 2 weeks, lift without recrudescent transferrer or reduce scope more remarkable.

结果和对照组比较，观察组术前CD4+T细胞、CD4+T/CD8+T值及NK细胞活性明显降低，而CD8+T细胞升高，和TNM分期有关；术后2周较术前CD4+T细胞、CD4+T/CD8+T值及NK细胞活性明显升高，而CD8+T细胞降低；术后4 a随访发现，上述指标变化趋向同术后2周，无复发转移者升高或降低幅度更显著。

Results Most OECs were bipolar or tripolar, and the purity was estimated to be over 88.7%. The non-OECs cells consists of astrocytes, neurons, oligodendrocytes and fibroblas.

结果 体外培养的新生小鼠嗅鞘细胞主要为双极或三极细胞，其纯度可以达到88.7%以上，污染细胞中有星形胶质细胞、神经元、少突胶质细胞和成纤维细胞。

RESULTS: Olfactory bulb-derived olfactory ensheathing cells adhered to the wall at 24 hours. Olfactory mucosa-derived olfactory ensheathing cells adhered to the wall at 4-5 days. The morphology of the two kinds of cells was similar. Most of them were bipolar and spindle-shaped, and few were tripolar and flat.

结果：体外培养24 h嗅球源性嗅鞘细胞即可贴壁，而嗅黏膜源性嗅鞘细胞多在培养四五天后贴壁，两种来源的嗅鞘细胞形态相似，以双极或梭形细胞为主，少量为3极及多突起形多级细胞，同时夹杂扁平、煎鸡蛋形细胞。

Flow cytometric analysis showed that after mock infection, Ad5null infection, and Ad5hSeFT infection, the mean fluorescence intensity values for the binding of Ulex europaeus Ⅰ lectin to NIH3T3 cells were 2. 3±0.6, 2. 1±1. 0, and 36. 5±5. 9, respectively; MFI values for the binding of Griffonia simplicifolia isolectin B4 (GS-IB4) lectin to NIH3T3 cells were 167±23, 170±19, and 100±14, respectively; MFI values for the binding of human natural IgG and IgM antibodies to NIH3T3 cells were 31±3, 32±4, and 22 ±4, respectively.

流式细胞分析结果表明，小鼠NIH3T3细胞及分别被Ad5null和Ad5hSeFT转导的NIH3T3细胞，UEA-1凝集素结合细胞的平均荧光强度值分别为2.3±0.6、2.1±1.0和36.5±5.9；GS-〓凝集素结合细胞的MIF值分别为167±23、170±19和100±14：人天然IgG和IgM抗体结合细胞的MFI值分别为31±3、32±4和22±4。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。