细胞

Result In the HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT, expressions of hTERT, Bcl-2 and mitochondrial cyt C were significantly down-regulated, while Bax and cytoplastic cyt C were obviously up-regulated, and active caspase-9 was found in addition to procaspase-9 compared with that in negative control cells and untransfected cells.

结果 与未转染细胞和对照细胞相比，转染细胞hTERT、Bcl-2和线粒体cyt C表达明显减少，Bax和胞质cyt C表达明显增加；未转染细胞及对照细胞仅见约46kD的前caspase-9条带，转染细胞可见46kD前caspase-9条带和35kD caspase-9 p35亚基条带。

We examined the properties of DC associated with immune suppression in NOD mice by insulin injection subcutaneously and ability to suppress diabetes transfer by diabetogenic effect cells in secondary NOD severe combined immunodeficiency recipients.

NOD小鼠皮下注射牛胰岛素诱导免疫抑制，以血糖检测、胰腺组织学分析和胰岛beta细胞凋亡分析观察自身抗原的免疫保护作用，分离骨髓树突状细胞细胞，流式细胞术分析细胞表面抗原，混合淋巴淋巴细胞反应测定细胞功能。

Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.

克隆大鼠NGF、BDNF和NT3基因，构建真核表达载体；3种重组载体分别转染星形胶质细胞，G418筛选后获得阳性克隆细胞进行扩大培养；取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞；用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。

The expression of nestin antigen, NSE and GFAP were identified by immunohistochemistry, and the number of NSE-positive cells and GFAP-positive cells were dividedly counted.

结果大鼠胚胎神经管神经上皮干细胞在无血清培养基中可形成大量呈nestin抗原阳性细胞构成的神经球，经传代有血清培养后分化为NSE阳性和GFAP阳性细胞，其中NSE阳性细胞占细胞总数的47.7％，GFAP阳性细胞占细胞总数的39.8％。

This topic investigate the change of epidermic cell in suspension and in simplex after they were preserved in defferent ways. The junction between cells and the adhesion between cells and matrix have important roles in the hypothermic impairment of tissue.Their existence affect the recovery of cells under hypothermia. Consequently,the cytoskeleton system may contribute to the impairment caused by junctions.

本研究利用体外培养的表皮细胞单层和单细胞悬液，采用不同的保存方法进行保存，观察到组织细胞间的连接以及细胞与细胞基质的连接对组织冷冻的损伤有重要的作用，它们的存在直接影响组织细胞的活力，其原因与细胞骨架系统有关。

He expression levels of TGF-β receptor 1 and TGF-β receptor 2 decreased in descending order of epitenon tenocytes (29.9%, 26.2%), endotenon tenocytes (27.8%, 23.5%), and tendon sheath fibroblasts (23.1%, 20.0%).

GF-β受体1、2 表达平均降低程度从大到小依次为腱外膜细胞（29.9%、26.2%），腱内膜细胞（27.8%、23.5%），腱鞘细胞（23.1%、20.0%）；TGF-β受体3 依次为腱内膜细胞（26.1%）、腱外膜细胞（19.2%）、腱鞘细胞（15.8%）。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现：小线角木蠹蛾性信息素分泌腺位于腹部末端8～9节之间，是一个由节间膜特化而成的上皮结构，为一可外翻的腹褶，腺体表面分布着饱满的锥形体，羽化后2天未交尾的雌蛾腺体细胞呈单层排列，腹面中央由密集的柱形上皮细胞组成，细胞排列向两侧延伸至背部，其形状由柱形逐渐变为扁平形，细胞核为椭圆形，细胞与细胞间有明显的胞连接，细胞基底膜基褶较多，质膜上分布着微绒毛，并与内表皮连接，内表皮上有多层几丁质，外角质层染色较深，细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

The protein synthesis of Ceteuisiae fermentum cell was inhibited by NCS when they were cultured in the medium with ~(35)S-Met.3. The Ceteuisiae fermentum cells treated with NCS were mostly in G1 stage during the cell cycle measurement. The amount of cells in Gl stage increased about 20% compared to the control. These data suggested that the process of protein synthesis may be inhibited or prolonged by NCS.

NCS处理下，观察酵母细胞周期时发现，多数细胞处于细胞周期的G1期，G1期细胞数目比对照多约20%，暗示NCS存在时，酵母细胞中的蛋白质合成过程延长或受抑制，进一步研究发现NCS对酵母细胞的凋亡率基本没有影响。

Results: In the first group of which pancreas was allotransplanted combining with spleen and duodenum: dropsy of stroma between pancreatic folia, light dropsy of gland alveolus cells and pancreatic islet cells were observed at 3 d post operation; Infiltration of inflammatory cells in pancreatic tissue and focus necrosis of the gland alveolus cells occurred at 5 d post operation; Necrosis and dissolve at part of gland alveolus cells and pancreatic islets were observed at 7 d after operation.

结果： 胰、脾、十二指肠联合移植组：术后3 d胰腺小叶间质水肿，腺泡细胞和胰岛细胞轻度水肿；术后5 d炎细胞浸润，腺泡细胞灶状坏死；术后7 d腺泡细胞和胰岛细胞部分坏死、自溶。

Fibroblasts have the ability to secret many kinds of extracellular matrix and cytokines relevant to hepatogenic differentiation. So, we investigate whether coculture of hADSCs with fibroblasts without cell-cell contact play a role in hepatic differentiation of hADSCs by RT-PCR analysis of hepatic specific gene expression and real-time PCR analysis of Alb expression.

成纤维细胞能够分泌很多利于干细胞向肝细胞分化的细胞外基质和细胞因子，我们采用非接触共培养的方式对成纤维细胞与人脂肪干细胞共培养能否引发其向肝细胞的分化进行了研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。