细胞

Methods SH-SY5Y cells, a human neuroblastoma cell line, were incubated with different concentrations of fluoride for 48 hr and somle of them were treated with vitamin E precedently. The functional situation of cells was measured by MTT method; lipid peroxidation was detected by High-Performance Liquid Chromatography; phospholipid was separated by a Silica SepPak cartridge and neutral lipids by HPLC.

体外培养SH-SY5Y人脑神经母细胞瘤细胞，在培养液中加入不同浓度的氟化物或加入抗氧化剂，培养48h后用测定细胞MTT的方法来了解细胞的损伤程度，用高效液相色谱法分离和测定培养液中脂质过氧化物水平，用过柱和比色法测定细胞生物膜磷脂含量，用高效液相色谱法测定细胞生物膜辅酶Q和胆固醇含量。

Results (1)Astrocytes and oligodendrocytes in mixed culture appeared better growth and differentiation than the respectively purified neuroglial, especially for the oligodendrocytes.(2) There were many pseudopod process among the astrocytes, which appeared centripetal growth mode.(3) The passaged astrocytes kept good abilities of division and proliferation and completely fused after 1 week of growth, and the purity was over 95%.

结果 (1)混合培养的星形胶质细胞和少突胶质细胞生长分化状况均优于纯化后的生长，以少突胶质细胞表现更为明显；(2)星形胶质细胞呈向心性生长，细胞相邻的接触面有较多的伪足；(3)传代培养的星形胶质细胞保持较强的分裂增殖能力，可在1周左右生长至完全融合，其纯度可达95%以上。

Using GSA-〓 lectin histochemistry, the first appearance of microglia occurred at E13, usually stained as scattered amoeboid cells. From then on, the number of microglia increased steadily, reached a peak at postnatal day 7 (P7), and then reduced gradually to adult level. Generally the morphological form of microglia undergoes transformation from early rounded or ameboid to adult resting ramified as they differentiate during development.

使用凝集素GSA-〓亲和组织化学染色，本实验最早观察到小胶质细胞在胚胎13天（E13），为散在分布的圆形或阿米巴样细胞，随后细胞数量逐渐增多；总体而言小胶质细胞的形态遵循着由圆形或阿米巴样逐渐转向纤细分枝状的演变规律，不同阶段细胞形态各有其特点，在不同区域也存在差异；小胶质细胞的数量在生后第七天（P7）达到高峰，其后开始逐步降低直至成年。

The analytical results above showed that the three indicators of DNES cells appear in different time and place of the sacculus rotundus.

这说明圆小囊中DNES的各种细胞成分存在着发育时间差；从免疫相关细胞成分来看，巨噬细胞和NK细胞在兔出生当日就有，而T细胞、B细胞则在5天以后才出现。

Methods Morphologic change of cells were observed with fluorescence microscope;MTT assay was used to evaluate survival rates Survival rates of GLC-82 cells;The influence of NA to cell migration was analyzed by scarification test; The percentage changes of apoptosis and the distributional percentage of cell cycle were analyzed with flowcytometry.

荧光显微镜观察细胞形态的变化；MTT 法检测不同浓度烟酰胺培养细胞后细胞的存活率；划痕试验检测不同浓度的烟酰胺对细胞愈合力的影响；流式细胞仪检测细胞周期和凋亡率。

Except the incomplete maturation of spermatid nuclear and oocyte activation, idendification of a live spermatid is a pivotal procedure. It is difficult to distinguish round spermatids from other round cells such as spermatocytes, monocytes, polymorphonuclear leukocytes and so on.

除精子细胞的核蛋白不完全成熟及卵子激活不足等因素外，如何正确选择存活精子细胞是个难题，如圆形精子细胞与精母细胞、单核细胞和多形核白细胞等其他圆形细胞的区分就比较困难。

Results The hela cells which transfected with HSV-TK and IL-12 gene was obviously changed in appearance after 24 hours which added GCV, but the normal control cells growthed prosperity. The survival rate of the hela cells which transfected with HSV-TK and IL-12 gene was fall-off with the plus of tile GCV. The killing effect of GCV for the hela cells transfected with HSV-TK and IL-12 gene was more strengthener than that for the normal cells.

结果 加入GCV后体外培养联合基因转染的Hela细胞，24h后细胞明显发生形态上改变，正常对照组细胞生长旺盛；随着GCV剂量的加大，转染联合基因的Hela细胞的存活率呈逐渐下降的趋势，GCV对转染细胞的杀伤作用较对正常细胞的杀伤作用明显增强。

METHODS: CNE-2 human nasopharyngeal carcinoma cells were exposed repeatedly to γ-rays and then screened for a new radioresistant subline named CNE-2R. Double times of CNE-2 and CNE-2R cells were determined by MTT assays. The relative radiosensitivities of tumor cells were assessed by standard colony formation assays. Survival rates after exposed to irradiation were measured by MTT assays. The time courses of cell cycle distribution before and after irradiation were investigated by flow cytometry.

用γ射线反复照射CNE-2细胞，筛选出放射抗拒细胞株CNE-2R；检测细胞生长的倍增时间；应用细胞克隆形成实验检测细胞的放射敏感性；MTT法检测照射后不同时间的存活分数；用流式细胞术检测细胞的周期分布特征。

The results showed that both CDV strains could induce apoptosis of infected cells. However, there was significant difference in the percentage of apoptotic cells between the two strains (P＜0.01). The strain resulted in the CPE type of cell-rounding induced much higher ratio of apoptotic cells than the strain resulted in the CPE type of syncytium.

结果发现，2种毒株感染引起的细胞病变类型不同，分为圆缩型与合胞体型；2种毒株均可诱导感染细胞凋亡，但毒株间的凋亡细胞比例存在差异，即产生合胞体型细胞病变的毒株凋亡出现较晚，凋亡细胞比例显著低于产生圆缩型细胞病变的毒株(P＜0.01)。

On the functional differentiation,we found and that the type II ras-GTPaseactivating protein (p100-GAP),which was specifically expressed in humanplacenta,was largely expressed in the highly-differentiated syncytiotrophoblastcells and moderately expressed in the cultured intermediate trophoblast cells.While in the non-differentiated cytotrophoblast cell line-NPC,no expression was observed.Its mRNA and protein expressingcharacterization was in consistent with that of hCGβ,which was one of the mostimportant markers of the trophoblast cell functional differentiation.In addition,the expressing amount of p100-GAP increased with the progressing of pregnancyand the syncytium formation in vitro.

细胞功能分化的研究结果表明：经重组质粒的构建获得特异表达于人胎盘的p100－GAP的cDNA探针后，从蛋白和分子水平证实p100－GAP大量表达于分化程度高的合体滋养层细胞，少量表达于体外培养的中间型滋养层细胞，而非分化的细胞滋养层细胞NPC则不表达，这种表达特性与作为滋养层细胞功能分化重要指标之一的hCGβ高度一致；p100-GAP表达量还随妊娠过程和体外合体化过程而逐渐增加。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。