细胞

The cells were passaged no more than 5 generations,and the cell freezing density is about 3×106 cell/ml, sample numbers of each kind are between 30 and 60, conserved numbers are 150-180 ampules,biological characters of fibroblast were analysed according to the request of constructing cell line. The results were as follows: 1 Cultured cells'morpha is typical fibroblast,which is fusiformate or unregular triangle, cells is radiant,flaming or rotative during growing .Qingyuan flesh chicken's cytoplasmic tubercle is much more and shorter in contrast with Zang chicken and Langshan chicken,that can be regarded as different character from others.

细胞冻存代数在5 代以内，冻存密度为3×106个细胞/ml，每个品种的样本含量在30～60 个之间，保存管数达150～180 支，根据建系要求，对成纤维细胞的生物学性状进行分析，所得结果如下： 1 培养细胞形态为典型的成纤维状，为梭形或不规则三角形，细胞在生长时呈放射状、火焰状或旋涡状走形，清远麻鸡的细胞形态与藏鸡和狼山鸡相比，胞质向外伸出的突起较多，且较短。

Representation of the world, mainly in the placental extract, Switzerland and Japan Placenta with a high purity, high-quality and high security features, extracted from the cells, the completion of injection can be divided into three stages: First, using the most advanced chemical and biological equipment, from the sheep embryo to extract the liver activity of the protein to remove impurities, heat-sensitive protein and can cause allergic reactions of the various types of risk factors, followed by the adoption of advanced technology on the extraction of viable cells were isolated, and thus be the activity of cells with different efficacy, such as the thymus cells, placental cells, liver cells and so on dozens of different types, the final freeze-dried using the most advanced technology to help keep cells alive nature.

胎盘素具有高纯度、高品质和高安全性的特点，从细胞的提取到针剂的完成可分为三个阶段：首先运用最先进的生化仪器，从小羊胚胎的肝脏中提取活性蛋白质去除杂质、热敏感蛋白质及可能导致过敏反应的各类危险因子，其次通过先进的技术对提取的活性细胞进行分离，从而得到具有不同功效的活性细胞，如胸腺细胞、胎盘细胞、肝脏细胞等几十种不同类型，最后采用目前最先进的冻干技术来保持细胞鲜活性。

RESULTS: The microscopic observation showed chondrocytes in the transducted group proliferated stably, and triangle, and spindle cells were predominant in the seventh passage, accompanied by few long-spindle and root cells. In addition, the cells were of clear appearance and large lucency. However, in the non-transducted group, long-spindle cells were observed in the fourth passage, and almost all cells exhibited long-spindle shape in the fifth passage.

结果：①倒置显微镜观察，转导组兔关节软骨细胞生长增殖稳定，至第7代仍旧以三角形、梭形及纺锤形细胞为主，偶见长梭形和树根形细胞，且轮廓清晰，透亮度大，折光性强；而未转导组细胞培养至第4代时即己出现长梭形细胞，自第5代细胞形态几乎全部变成长梭形。

Results In the medium and high dose F0 groups, it was observed that the atrophy and incrassation of seminiferous tubule, decrease of spermatogenesis, hyperplasia of interstitial tissue, especially in high dose groups spermatozoon abnormality and nucleolus concentration in the rats testis after DU ingestion for 14 months. The changes became more severe with the prolongation of DU ingestion. Such changes occurred in filial rats (F1) after DC ingestion for 5 months. In the medium and high dose F0 groups, it was observed that a little atrophy of kidney glomerulus, hyperplasia of interstitial tissue after DC ingestion for 14 months, and kidney glomerulus fibrosis happened after DC ingestion for 20 months, such changes occurred in filial rats (F1) after DC ingestion for 5 months In the medium and high dose F0 groups, splenic germinal center and periarterial lymphatic sheaths were hyperplasia , companies with lymphopoiesis after DC ingestion for 7 months, splenic white pulp became more small and sparse after DC ingestion for 20 months.

结果 F0代的中、高剂量组大鼠摄入贫铀14个月后可见雄性的精曲小管萎缩，管壁增厚呈空虚网状，生精细胞层次减少，间质细胞增生，但仍见有精子生成；高剂量组可见到精子呈异型性改变，细胞核浓缩深染，且随着摄入时间延长改变愈趋明显；F1代大鼠摄入贫铀5个月后就有上述改变且更为严重。F0代中、高剂量组大鼠摄入贫铀14个月后肾小球轻度萎缩，间质增生明显，20个月时肾小球萎缩纤维化；F1代大鼠摄入贫铀5个月后就有上述改变。F0代中、高剂量组摄入贫铀7个月时脾脏生发中心和淋巴鞘增生，淋巴母细胞增生活跃，20个月时脾小体减少，生发中心稀疏；F1代大鼠摄入贫铀早期和晚期有类似改变。F0和F1代高剂量组摄入贫铀早期肝脏有炎症细胞浸润，晚期骨髓有核细胞减少，脂肪细胞增加。

And the repression of NO-induced cell death with Ppbi-1 overexpression in transgenic lines was apparent even at SNP concentrations up to 1mM.In addition work,we found that most of suspension cells treated with 0.5mM SNP can be stained by Sytox green,some PCD morphologic characteristics such as chromatin condensation and margination can be observed.DNA Ladder was also detected with these cells.As to the control and the cell treated with 0.5mMSNP+20μM Est,the above-mentioned characteristics can\'t be detected.

对0.5mM SNP和0.5mM SNP+20μM Est处理的飞廉转基因细胞培养三天后用特异性核染料sytox染色，0.5mMSNP单独处理的细胞大部分核被染色，而且细胞核出现了核凝聚、以及染色质边集等凋亡细胞的特征；提取细胞DNA电泳分析，观察到了DNA LADDER，这说明，该细胞可能已经发生凋亡，而Est诱导Ppbi-1基因表达的细胞均未出现这些凋亡特征。

So, the nucleocytoplasmic ratio decreased. 10^(-5)MATRA significantly suppressed the proliferation of os732 with only 39.5% cells when compared with untreated cells. Cells in G1 phase increased significantly, and the number of S phase decreased. Expressions of cy-clinD1, cycimD2 and CDK4 mRNA declined, especially cyclinD2 and CDK However, expressions of cyclinD3 and P21WAF1 mRNA changed little.

结果 ATRA作用后，OS732细胞胞浆展开，细胞核变小，核浆比例变小；瘤细胞生长减慢，细胞计数为正常对照组的39.5%； G0/G1期细胞明显增多，S期细胞减少，细胞阻滞于G0/G1期；CyclinD1、CyclinD2、CDK4mRNA表达均下降，尤以CylinD2、CDK4下降最明显，CyclinD3、P21WAF1mRNA未见明显改变。

Methods OS732 was treated with 10^(-5)MATRA for 6 days, then we observed cell morphology, counted cell number, analyzed cell cycles by flow cytometry and detected mRNA expressions of cyclinD (Dl, D2, D3), CDK4, P21 by semi-quantity RT-PCR. Treatmed with 10^(-5)MATRA, The nucleus of OS732 cells became small and condensed, and cytoplasms expanded.

用10^(-5)MATRA作用骨肉瘤细胞OS732， 6d后，进行细胞形态学观察、活细胞计数、流式细胞仪分析细胞周期、半定量RT-PCR检测细胞周期调控蛋白CyclinD(D1、D2、D3)、CDK4、P21 mRNA表达的改变。

In this study, we utilized NCCmelan5 and NCCmelb4 melanoblast, two melanoblast cell lines which were previously used to investigate the treatment of vitiligo, to investigate the anti-apoptotic effects of LED (630nm), and ruled out others causes of repigmentation including proliferation, mitochondrial metabolism, melanin production, migration distances of MBs after irradiation, to formulate the theoretical basis LED's treatment of vitiligo.

在此研究中，我们使用研究白斑常用的NCCmelb4 and NCCmelan5两株黑色素母细胞珠来研究LED 光源对黑色素母细胞的抗凋亡的细胞保护功能，并且排除其对於细胞直接造成细胞增生，细胞移动，或黑色素增加的机转，希望为其治疗白斑的应用提供依据。

In our study, we found menadione has anticancer potential on human breast adenocarcinoma MDA MB 231 by weather cell cycle arrest, anti proliferation, or apoptosis. Low concentration of menadione (5.8μM) significant inhibited growth curve on breast cancer cells (MDA MB 231) but not breast cells (MCF 10A) by performing MTT and trypan blue cell counting. RT-PCR and western blotting showed menadione increase p21 gene and protein expressions but decrease cyclin E and CDK2 protein expression resulting in cell cycle G0/G1 arrest by flow cytometry.

MTT及生长曲线的实验，发现menadione在低浓度且长时间作用情况下，对在於人类乳腺上皮细胞癌(MDA-MB 231)是有明显的抑制作用，而在正常乳腺细胞(MCF-10A)则没有影响，在分子转录上及蛋白质表现方面也发现低浓度menadione处理24时能使MDA-MB 231诱发p21基因及蛋白表现量增加及cyclin E、CDK2抑制，再经由流式细胞仪分析也发现低浓度 menadione 处理24小时能使MDA-MB 231细胞周期GO/G1比例上升，造成细胞周期停滞，进而抑制细胞生长。

Methods Primary metanephric mesenchyme stem cells were cultivated and multi-directional differentiation was induced.

方法原代分离培养后肾间充质干细胞，在体外特定诱导条件下观察细胞向成骨细胞、脂肪细胞及肾小管上皮细胞的分化及其形态演变，采用细胞免疫荧光检测细胞表面特定蛋白的表达变化。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。