细胞

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

Methods: The effect of MXC on proliferation and apoptosis of granulose cells was assayed by using MTT assay to measure the cell vitality, flow cytometer to detect cell apoptosis, electron microscopy to observe the morphological changes after the action of MXC.

MTT法测定卵巢颗粒细胞在MXC作用后的细胞活力；流式细胞技术检测细胞凋亡变化；电镜观察细胞的形态学改变等；综合评价MXC对卵巢颗粒细胞增殖和凋亡的影响。

At present,ICC-like cells similar to ICC in morphology and immunology is isolated and cultured from guinea-pig bladder,and it has many characters of pacemaker cells,of which the electrophysiology in relaxation and contraction of bladder is a key problem attracting more attention.That in morphology and physiology of the status quo were reviewed in This article.

目前已从豚鼠膀胱中成功地分离培养出了在形态学和免疫学上与ICCs 相似的细胞，被命名为"膀胱Cajal样间质细胞"，并发现这些细胞具有起搏细胞的某些特征，因而这类细胞的电生理活动与膀胱功能的关系成了广大科研人员关注的问题，本文就膀胱Cajal样间质细胞的形态学及生理学的研究现状进行了综述。

Preparation of ratiometric pH nanosensors and its appication in intracellular pH detection of the anti-tumor drugs treated Hela cells The ratiometric pH nanosensors that contained a pH sensitive indicator fluorescein isothiocyanate and a reference dye tris(2, 2′-bipyidyl) dichlororuthenium hexahydrate had been prepared with O/W microemulsion method.

利用该内参比pH纳米传感器尺寸小可以通过细胞的内吞而无损伤地进入细胞内的优势，进一步结合流式细胞学分析方法，对不同抗肿瘤药物作用的Hela细胞内的pH变化进行了分析，结果表明：不同抗肿瘤药物作用后的Hela细胞内pH的变化情况并不一样，有的作用后引起了细胞酸化，有的作用后引起了细胞碱化，有的作用后基本上不引起变化。

The GFAP is a specific marker of astrocyte, its expression is more higher in the activity astrocyte, and finally the GFAP become the main composition of scar formations. The S -100 is a kind of acidity, dissolubilites, low molecular quantity and calcium hydronium conjugated protein, and it is mainly existed in neuroglial cell and schwann cell. It can promote the growth of axon, glial hyper-plasia and nerve divide and calcium's stability inside of cell, thus regulatin g the shape and metabolism of astrocyte . The quantity of S -100 protein and the degree of ischemia have direct proportion .

胶质纤维酸性蛋白是星形胶质细胞的特异性标记物，在活性星形胶质细胞中GFAP的表达相对更高，且最后GFAP成为胶质疤痕的主要成份。S-100蛋白是一种酸性、可溶性、低分子量的钙离子结合蛋白，主要存在于胶质细胞和雪旺细胞中，它可促进轴突生长、胶质增生、神经分化和细胞内钙的稳定，从而调节星形胶质细胞的形态和代谢。S-100蛋白与缺血的程度是成正比的。

The cells were incubated in RPMI 1640 without or with different contents of glutamine and with 10%fetal calf sera, at 37℃,5% CO2 and saturation humidity for 4 days.The initial living cell density was 5×108/L. After 4 days incubation,the cells were counted,collected, smeard and stained with Wright-Giemsa, DNA, POX, NAE and NaF inhibition,gulping ink and NBT etc. cytochemical technology. The stained cells were investigated under oil immersion lens.

从18例未经治疗的APL病人外周血中提取APL原代细胞，在无Gln的RPMI 1640培养液中补加10%胎牛血清，以活细胞密度5×108/L接种细胞，在37 ℃， 5%CO2和饱和湿度下培养4 d，计数活细胞，并收集细胞行Wright-Giemsa、DNA、POX、NAE及NaF抑制试验、墨汁吞噬试验和NBT还原试验等细胞化学染色，于油镜下观察。

The results indicated that the content of cytosolic CaM in cells treated with exogenous Ca2+ has increased indistinctively, while fluorescence intensity in cells treated with TFP decreased. So we believed that exogenous Ca2+ has little effect on the expression of CaM. High concentration of TFP can enter yeast cells and combine to CaM to make it inactive, which is the reason that TFP restrain the growth of yeast cells. 5mmol/L EGTA could completely arrest the cell proliferation of MFP7 after 28h, when the fluorescence intensity in cells wasobviously increased with flow cytometry and LSCM.

被较高浓度TFP处理过的MFP7胞内荧光强度则相对较弱，推测是因为TFP进入胞内与CaM结合从而使其失活，这也是高浓度TFP抑制酵母细胞增殖的原因。5mmol／L EGTA处理28h左右后，酵母细胞的生长被抑制在G1晚期，此时通过细胞流式法和在激光扫描共聚焦显微镜下观察到胞内荧光强度显著高于对照，说明CaM的表达水平在G1后期开始上升；回加10mmol／L Ca~(2+)处理24h后，细胞开始恢复生长，细胞流式法测定胞内荧光强度有所下降，表明多数细胞完成G1／S转换，进入生长对数期。

Fund Project: the National Natural Science and Technology Source Program, No. 2001DEA1006*Abstract: Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have no cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。