细胞

Results Many endothelial cells were observed with higher vigor and adherance to the wall after incubation for 3-5h, and spreaded to form cell masses for 12-24h, and grew densely and converged into single layer which characteristically arranged to cobble-stone shape after incubation for 1-2 weeks.

结果 原代细胞培养3~5h后，大部分活力较高的内皮细胞已开始贴壁，12~24h后，内皮细胞己铺展形成由数个细胞组成的细胞群，1~2w后，细胞呈生长密集状并汇合形成单层，在倒置相差显微镜下，呈特征性"鹅卵石状"或"铺路石状"镶嵌排列生长。

Western-blot and immunohistochemical assay methods were employed to detect the expression of FHIT. Apoptosis and cell cycle of cell were analyzed by FCMS. Morphological changes were observed by compound and electron microscope.

然后用流式细胞仪，普通光镜及透射电镜对获得稳定FHIT蛋白表达的转染组细胞和空真核表达质粒转染组细胞及未转染的亲本细胞凋亡的状况进行了考察，另外还通过流式细胞仪检测了三组浙江大学硕士学位论文细胞生长周期的惰况。

The suspension cells of the heart at E11 were respectively cultured in co-culture system based in the stromal cells from the heart, the fetal liver and AGM region with the same conceptus age, and c-kit+ cells were analyzed by flow cytometer, estimating the effect of different stromal cells on HSCs\' amplification.

将培养24h后的E11心脏悬浮细胞分别置入由同期的心脏内皮细胞、胎肝基质细胞和AGM区基质细胞构成的共培养体系中，于0h， 24h和48h分别检测c-kit+细胞比率，研究各组织基质细胞对HSCs扩增的影响。

Antigene peptide nucleic acid specifically inhibits MYCN expression in human neuroblastoma cells leading to cell growth inhibition and apoptosis Roberto Tonelli,Stefania Purgato,Consuelo Camerin,Raffaele Fronza,Fabrizio Bologna,Simone Alboresi,Monica Franzoni,Roberto Corradini,Stefano Sforza,Andrea Faccini,Jason M. Shohet,Rosangela Marchelli,and Andrea Pession Department of Pediatrics, University of Bologna,Sant'Orsola-Malpighi Hospital, Bologna, Italy; Department of Organic and Industrial Chemistry, University of Parma, Parma,Italy; and 3Center for Cell and Gene Therapy, Texas Children's Cancer Center, Baylor College of Medicine, Houston, Texas Abstract We developed an antigene peptide nucleic acid for selective inhibition of MYCN transcription in neuroblastoma cells, targeted against a unique sequence in the antisense DNA strand of exon 2 of MYCN and linked at its NH2 terminus to a nuclear localization signal peptide.

抗原肽核酸抑制MYCN具体表达的神经母细胞瘤细胞，导致细胞生长的抑制和凋亡罗伯托利，斯特凡Purgato ，苏埃洛卡默琳，拉菲尔Fronza ，梅奥尼博洛尼亚，西蒙娜Alboresi ，莫妮卡弗兰索尼，罗伯托Corradini ，斯特凡诺斯福尔扎，安德烈Faccini ，杰森先生Shohet ， Rosangela Marchelli ，和安德烈Pession儿科，博洛尼亚大学，圣' Orsola -马尔皮基医院，博洛尼亚，意大利；有机部和工业大学化学帕尔马，帕尔马，意大利和3Center的细胞和基因治疗，德州儿童癌症中心， Baylor医学院，得克萨斯州休斯敦摘要我们开发了一种抗原肽核酸的选择性抑制神经母细胞瘤MYCN转录细胞，针对一个独特的序列反义DNA链的第2外显子，并与在MYCN 其氨基端为核定位信号肽。

Cell of Ela of 鳫 of fleeing Yu Piao, the stage longs for blue refus to catch a law to detect wither of cell of revulsive Hela of EVn-50 of observation of microscope of fluorescence of coloring of cellular vigor;AO/EB dies electrophoresis of gel of morphology change;DNA is corroborant wither of cell of EVn-50 revulsive Hela dies action;PI coloring sheds type cell appearance to detect wither of cell of EVn-50 revulsive Hela dies rate.

体外培养Hela细胞，台盼蓝拒染法检测细胞活力；AO/EB染色荧光显微镜观察EVn-50诱导Hela细胞凋亡形态学改变；DNA凝胶电泳确证EVn-50诱导Hela细胞凋亡功能；PI染色流式细胞仪检测EVn-50诱导Hela细胞凋亡率。

Result : 1. E xperimental study:(1)After 48 hours culture,the morphology of K562 cells that FFJZ's concentration is 1mg/ml and less than 1mg/ml became fusiform and claviform.The K562 cells that FFJZ's concentration is 2mg/ml has no conspicuous different.The K562 cells that FFJZ's concentration is 4mg/ml and 6mg/ml took on shapes of crenation.

结果：1、体外实验：（1）K562细胞经FFJZ作用48小时后，浓度在 1mg/ml及以下组出现细胞形态呈梭状、棒状，伴有胞浆丝状突起形成伪足，2mg/mlFFJZ组细胞与对照组无明显差别，4mg/ml及6mg/mlFFJZ组，细胞出现皱缩、破碎； 8mg/mlFFJZ及10mg/mlFFJZ组细胞经培养24小时后，即出现细胞明显变小，且均匀分散，以后时间里均呈现此种状况。

The animal experiment stage: 40 rabbits underwent corneal epithelium curettement and limbal ablation to induce limbal stem cell deficiency and were randomly divided into four groups, simple amniotic membrane group, non-frozen cells group, glycerol group and dimethyl sulfoxide group(n=10 for each group). Searching for the activity of frozen and non-frozen cells more completely by transplanting subculturing cells and observing the accomplishment of cells function.

动物实验阶段：将40只角膜缘干细胞损伤的模型兔随机分成单纯羊膜组、未冷冻细胞组、DMSO保存细胞组和甘油保存细胞组，每组10只，用传代培养2周后的角膜缘上皮细胞作移植实验，观察细胞体外功能完成情况来进一步比较深低温保存前后角膜缘上皮细胞的活性。

Then the changes of cell shape and ultrastructure were observed by microscope and transmission electron microscopy, and the percent of cells in the G1 phase and SA- ft -galactosidase activity were measured with flow cytometry and cytochemistry staining, respectively.

从光镜、透射电镜观察细胞形态及超微结构的变化；流式细胞术分析细胞周期，计算G1期细胞的比例；SA-β-半乳糖苷酶的细胞化学染色，确定成功建立体外细胞衰老的模型。

Microcells were produced by centrifugation of micronucleated A9 (neo12) cells in Percoll density gradient containing 20 mg/L Cytochalasin B at 39 000 g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 μm and 5 μm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20 mg/L细胞松弛B的Percoll密度梯度介质中，经39 000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8 μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

Microcells were produced by centrifugation of micronucleated A9 (neol2) cells in Percoll density gradient containing 20mg/L Cytochalasin B at 39 000g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 pm and 5 pm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20mg/L细胞松弛B的Percoll密度梯度介质中，经39000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐