细胞

Transgenic CD34+ cells in the experimental group still could generate brust forming unit-erythroid, colony-forming units granulocute/monocyte and multipotential hematopoietic progenitors; especially, CFU-GM was the main cell in hemopoietic progenitor cell.

实验组转基因CD34+细胞于12周后仍可产生造血祖细胞集落红系祖细胞、粒单系祖细胞、脐血多向造血祖细胞，所形成的造血祖细胞集落以粒单系祖细胞为主。

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。

Long-term aerobic exercise can effectively prevent hepatocyte number from decreasing accompanying with aging. Among the groups of aged, results showed that rats of 1-hour and 2hours group whose recovery function of hepatocyte apoptosis were of most significant. The increasing number of hepatocyte might be one of the cytological mechanisms of how aerobic exercise delays cell aging. Further researches on the phenomenon of the anomalous change of the hepatocyte apoptosis ratio among the youth groups are needed expectably.

肝细胞凋亡存在随增龄明显下降的趋势，这对维持肝细胞数量或缓解增龄过程中肝细胞数目丢失具有十分重要的意义；长期有氧运动可以有效的阻止肝细胞凋亡率在增龄过程中下降的趋势；在老年前期组中，训练1小时和2小时组对肝细胞凋亡能力的恢复作用最明显；有氧运动促进肝细胞凋亡能力的提高可能就是有氧运动延缓细胞增龄发生和发展的重要细胞学机制之一。

Some of transplanted cells expressed titin and the assembled titin could be detected in the cells adjacent to host myocardium. Cx43 could be observed between these cells. But the size and structure of transplanted cells were different from normal contractile myocardium.3. Most of the grafted cells were isolated by fibrous tissue. Part of these cells differentiated into fibrocytes. The structure of host myocardium was slightly disturbed.

MSCs植入正常心肌组织后能存活至12周，部分移植细胞表达titin，且与宿主心肌细胞相邻的细胞表达titin组装程度更高，随时间延长表达逐渐明显，其后进入平台期，但细胞体积及胞浆发育均不及正常心肌细胞；在移植细胞与宿主细胞之间可检测到Cx43的表达。

Methods: By the adherence , to isolate the bone marrow mesenchymal stem cells and adipose mesenchymal stem cells , to compare the characteristics of the two cells , including obtain the quantity of the cells 、 the morphos of the cells 、 the rate of adherence 、 cell kinetics 、 cell surface marker .

利用细胞贴壁性对骨髓间充质干细胞和脂肪间充质干细胞进行分离，比较两种细胞的情况，包括(1)单位质量组织获得两种细胞的量、(2)细胞形态、(3)细胞贴壁率、(4)细胞动力学、(5)细胞表面标记。

Since an excessive cell growth is considered as the main pathological event, cell proliferation model occupy most of the animal models, and these cells include retinal pigment epithelial cell, fibroblast cell, cartilage cell and vascular endothelial cell.

由于PVR的主要病理变化是细胞的过度增生，因而动物模型多以细胞增生模型为主，细胞种类有视网膜色素上皮细胞、成纤维细胞、软骨细胞、血管内皮细胞等。

Can reverse JA-induced migration inhibition JA-treated cells also showed significantly higher adhesion to laminin In conclusion these results indicate a novel anti-tumor role of JA via reducing reorganization of cytoskeleton increasing integrin 3 expression and adhesion ability to inhibit tumor cells migration Furthermore immunofluorescence staining of phalloidin/actin indicated that JA-treated cells exhibited cytoskeleton rearrangement and significant decreases in the number and length of filopodia Activity of Rho-family small GTPases was detected by a GST-pull down assay and showed that GTP-loaded Cdc42 and Rac were decreased in JA-treated cells In the future we hope these findings will be helpful to further understand the mechanisms underlying tumor progression in oral cancer and subsequently improve the cancer prevention and treatment

以抗体阻断integrin ？3可以有效使OC2恢复被JA所抑制的迁移能力。细胞贴附实验指出JA能提高OC-2在laminin环境下的贴附能力。当我们进一步以免疫萤光染色观察细胞，发现在JA处理下的OC-2，其外形、细胞骨架和细胞边缘的filopodia的密度和长度明显减少，利用GST-pull down assay侦测到Cdc42-GTP和Rac-GTP的表现量降低。推论爵床素A可能透过刺激口腔癌细胞大量表现integrin ？3，使细胞贴附能力增加或是透过改变细胞骨架来降低细胞迁移能力。希望本研究对於JA所做的抗癌及抗转移效应评估能提供口腔癌治疗在研发上一些帮助。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型；以硫氢化钠作为H2S的供体；应用CCK-8比色法检测细胞存活率；碘化丙啶染色流式细胞技术检测细胞凋亡率；Hoechst33258染色检测细胞凋亡的形态学变化；罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位；2'，7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

Cellular adhesion molecule is a kind of glucoprotein expressed on the membrane surface of various cells,which mediate the conjunction and adhesion of cells and extracellular matrix.Vascular cellular adhesion molecule-1(VCAM-1) and intercellular adhesion molecule-1 (1CAM-1) are members of CAM.

细胞粘附分子(cellular adhesion molecule，CAM)是一类由多种细胞产生的，调节细胞和细胞间以及细胞和基质间相互结合并起粘附作用的膜表面糖蛋白，主要包括血管细胞粘附分子-1(vascular cellular adhesion molecule-1，VCAM-1)和细胞间粘附分子-1(intercellular adhesion molecule-1，ICAM-1)等。

Retinotopy; color sensitivity; color-selective region; lateral geniculate nucleus; visual cortex; area V8; area V1; cones; visual pigments; fovea centralis; macula lutea; red; green; blue; Young-Helmholtz theory; short-wavelength-sensitive cones; medium-wavelength-sensitive cones; long-wavelength-sensitive cones; S cones; M cones; L cones; visual perception; ectostriatum; tectofugal visual pathways; thalamofugal visual pathways; color-reversal learning; visual Wulst

retinotopy；感色灵敏度；颜色-选择区；侧膝体核；直观皮层；区域V8；区域V1；圆锥细胞；视色素中央凹；黄斑；红色；绿色；蓝色；三原色学说；短波长敏感圆锥细胞；中波长敏感圆锥细胞；长波长敏感圆锥细胞；S圆锥细胞；M圆锥细胞；L圆锥细胞；视觉；ectostriatum；tectofugal视路；thalamofugal视路；颜色-倒序学习；visual Wulst

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐