细胞

The frequency value in S phase is 4. 83 times of M phase, 2. 9 times of G1 phase, and 3. 41 times of unsynchronized cells. These results suggested that gene correction events were highly related to S phase. Then we compared the targeting efficacy for two groups of cells in S phase. One group was contact with thymidine and the other one went smoothly through S phase without replication blockage.

基于这种原因，我们对基因定点修复事件做了细胞周期定位分析，发现同步于S期的细胞群发生定点修复的效率明显高于其它各期以及非同步化的细胞，流式细胞仪检测S期定点修复效率为M期细胞的4.83倍，为G1期细胞的2.9倍，是未同步化细胞的3.41倍。

Before the two-hour interaction of male-male individuals , we found there were obviously fewer and lighter stained ERp immunoreactive neurons (ER|3-IRs) in both main olfactory bulb system project regions and vomeronasal system project regions in the mandarin voles than in the reed voles, but no significant difference in numbers of AR immunoreactive neurons between the two species through the examining of immunoreactive neurons, moreover, there were significantly fewer AR-IRs than ERp-IRs in two species of vole before the two-hour interactions.

免疫组织化学检测，没有社会交往时棕色田鼠主嗅球系统投射区和犁鼻系统投射区ERβ免疫阳性细胞明显少于沼泽田鼠，且显色淡，AR免疫阳性细胞在两种鼠间差异不大，且都明显少于各自的ERβ-IRs.2h交往后，棕色田鼠主嗅球投射区和犁鼻系统投射区的ERβ-IRs细胞数明显少于交往前，AR-IRs细胞数明显多于交往前；沼泽田鼠交往前与交往后ERβ-IRs和AR-IRs细胞数均无显著差异，且显著多于交往后棕色田鼠ERβ-IRs细胞数，显著少于交往后棕色田鼠AR-IRs细胞数。

The cell cycle and apoptosis and the expression of P-pg, bcl-2, P53 in the MG63 and MG63/R cell lines were analyzed by flow cytometry. Results The resistance index of the MG63/R cells to cisplatin was 83.557±4.841. The cells also had resistance to doxorubicin, vincristin, methotrexate and cyclophosphamide. Disordered structure of the MG63/R cells was observed through microscopy. The cells appeared in triangle, polygon and polynucleation. The increase of granular endoplasmic reticulums and apophyses was observed through transmission electron microscopy. The proliferation ability of MG63/R increased significantly, with a low apoptosis index.

结果 经顺铂186d的诱导，建立了MG63/R，其对顺铂的耐药指数为83.557±4.841，对阿霉素、长春新碱、氨甲基蝶吟、环磷酰氨亦产生不同程度的交叉耐药；光镜观察可见MG63/R细胞排列不规则，形态呈三角形、多角形及多核现象；透射电镜显示MG63/R细胞表面突起增加，粗面内质网丰富；细胞周期分析显示细胞增殖能力明显增加而细胞凋亡明显降低；MG63/R细胞 P-pg、bcl-2阳性表达较MG63细胞明显增加，P53表达则明显降低。

Results Positive staining for VEGF was distributed mostly in osteoblasts, osteoclasts and newly-born osteocytes. Vascular endothelial cells and bone marrow cells also showed VEGF positive reaction. Hypertrophic chondrocytes in calcifying region of chondroephysis were VEGF positive, but VEGF was absent from chondrocytes in other zones of chondroephysis.

结果 VEGF阳性反应主要见于成骨细胞、破骨细胞及新生骨细胞中，血管内皮细胞及骨髓细胞也呈阳性反应；骺软骨钙化区肥大的软骨细胞为VEGF阳性反应，但其它区域的软骨细胞中无VEGF表达。

Results: There were normal appearances of the morphology, volume and microscopic demonstration of both right and left hepatic lobes in sham-operating control group. In HA+PV group, hepatic right lobes were coagulatively necrosed. There was necrosis of liver cells in each sub-groups, accompanying with a few apoptic cells in the 3h sub-group. Along with the time extending, necrotic cells were increasing, and apoptic cells were decreasing. Necrosis in right lobes was at the most at 72h group.

结果：假手术对照组肝左右叶形态、体积以及各种病理学检查均未见异常；HA+PV组术后肝右叶发生凝固性坏死，3h即可见点片状肝细胞坏死，内见少量凋亡细胞，随时间延长，凋亡细胞减少，坏死细胞增多，至72h坏死更加彻底：PV组术后24h肝右叶萎缩，电镜及TUNEL均显示3h组织内可见凋亡细胞，至24h凋亡细胞数达高峰，72h凋亡细胞减少，出现局灶性坏死。

The former formed a kind of KR cyst, while the later formed a kind of NKR cyst. All the kinetosomes, ciliary shafts and microtubules were resorbed in the former, meanwhile, the cell lost most water and cell volume decreased distinctly. In the later, only part of kinetosomes and part of microtubules were resorbed. Cell lost water slightly and volume decreased slightly.

期间，细胞吸收了全部的毛基体、纤毛杆和微管结构，伴随着细胞剧烈的失水浓缩，细胞体积显著缩小；游仆虫在形成毛基体非吸收型包囊时，细胞仅经历了部分的脱分化过程，期间，细胞仅吸收了部分的毛基体及部分微管结构，并且细胞仅发生了轻微的脱水和浓缩，细胞以折叠的形式容纳在包囊壁内。

In vivo, CXCR4-A549 and A549 cells were injected subcutaneously into nude mice(each group in 6).Four weeks later the mice were scarified and the tumors in situ and the lungs were taken out to be examined histologically.(3) Th cell cycle distribution was measured by flow cytometry to analyse the effect of SDF-1/CXCR4 in the cell proliferation and atoposis. The changes of the intracellular calcium concentration after SDF-1 activation were aslo measured by flow cytometry. Western blot was used to anlayse the phosphorylation of AKT and ERK, and the expression of MMP-2 and VEGF-C.

3为探讨SDF-1/CXCR4在肺癌侵袭和转移中的作用机制：①利用流式细胞仪检测CXCR4-A549与A549两组细胞的增殖和凋亡，分析上调CXCR4的表达后，对细胞生长周期的影响；②利用流式细胞仪检测两组细胞钙离子内流强度，分析SDF-1/CXCR4对钙离子通道活性的调控；③采用Western blot实验检测两组细胞PI3K/AKT、MAPK/ERK相关信号通路的活性，分析SDF-1/CXCR4可能激活的下游信号分子；④采用Western blot和RT-PCR检测两组细胞肿瘤转移相关基因VEGF-C、MMP-2的表达，探讨SDF-1/CXCR4与VEGF-C、MMP-2的相互作用关系。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

The capacity of endothelial cells to recognize and migrate in response to the extracellular matrix depends on one or more members of the integrin family of cell adhesion receptors.

血管新生的过程依赖于参与血管生成的细胞因子和细胞粘附的协同作用，整合素是一组细胞表面受体，介导细胞-细胞间、细胞-基质间的相互作用，对内皮细胞的粘附、迁移、增殖起重要作用。

METHODS: Using tissue culture technique, the maximal non-toxic concentration of luteolin in swine testicle cell culture was measured, and the inhibitory effect of luteolin on TGEV was measured by cytophathogenic effect observation and crystal violet measurement. In addition, the degrees of inhibition of the luteolin on CPE of virus in cell culture were observed by changing sequence of adding the vires and the drag. RESULTS : The maximal non-toxic concentration of luteolin was 873.39 μmol/L.

采用细胞培养法，通过结晶紫摄入量测定结合细胞病变作用，观察木犀草素在不同的加药方式下，对感染TGEV的猪睾丸细胞的保护作用、结果：TGEV病毒先吸附ST细胞再加入药液，先与TGEV病毒孵育再接种于卯细胞，或药液先与细胞孵育再用病毒感染等不同方式下，木犀草素在0.84-54.50μmaol／L浓度范围内，均能明显地提高细胞的存活数。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。