细胞

Morphological and flourescent changes were assessed using confocal microscopy in whole-mount organ of Corti preparations. Results (1) After the animals were exposed to broadband noise at 122 dB SPL in 4 h/day for 2 days, both apoptosis and necrosis appeared in OHCs. The single strand DNA in apoptotic OHCs were observed both in guinea pigs and mice.(2) In normal OHCs, EndoG was distributed outside of nuclei. EndoG translocated from outside to inside of the nuclei in both apoptotic and necrotic OHCs following noise exposure.(3) The MNNG cochlear perfusion and noise exposure both caused the transloctation of AIF from the mitochondria to the nuclei. The translocation of AIF took place in both apoptotic and necrotic OHCs.

结果 (1)暴露于120 dB SPL的白噪声环境中每天4小时，连续2天后引起豚鼠和小鼠耳蜗外毛细胞凋亡时，其细胞核内产生ssDNA，而在正常细胞内没有三ssDNA；（2）在正常情况下，EndoG分布于耳蜗毛细胞的细胞核外，在暴露于上述噪声后发生凋亡和坏死的豚鼠耳蜗外毛细胞中，EndoG从细胞核外转移到细胞核内，细胞核中的EndoG显著增加；（3）豚鼠耳蜗外淋巴灌流烷化剂MNNG后发生耳蜗外毛细胞凋亡和坏死，在凋亡和坏死的耳蜗外毛细胞中，AIF自线粒体转移到细胞核，其变化与噪声损伤引起耳蜗外毛细胞凋亡和坏死时一致。

The mechanism probably is that Pishang can inhibit the growth, movement and adheresion to extracellular matrix and endothelial cell of liver tumor cells, up-regulate the expression of nm23, down-regulate the expression of CD44, MMP-2, and inhibit the tumor neovascular by supressing the vascular endothelial cell, down-regulating the expression of VEGF, damaging the primitive mesenchymal cells and inhibiting neovascular gemmation.

砒霜具有明显的抗肝癌细胞作用，并有一定的抗侵袭与转移作用，其机制可能与其能抑制肝癌细胞增殖、迁移、阻止肝癌细胞与细胞外基质和内皮细胞粘附、上调肿瘤转移抑制基因nm23表达、下调肿瘤侵袭分子CD44、MMP-2表达以及通过抑制内皮细胞增殖、下调VEGF表达、损伤间充质细胞、阻止新生血管芽生等途径从而抑制肿瘤新生血管形成有关。

The study was purposed to investigate the effect of extract of Agkistrodon Halys venom on proliferation and apoptosis of K562 cells. The inhibition of K562 cell proliferation was measured by MTT assay; The morphologic changes of K562 cells was observed by microscopy; the apoptosis of K562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase in K562 cells was detected by Western blot.

本研究探讨皖南蝮蛇毒蛋白提取物对白血病细胞K562增殖的影响及诱导凋亡的作用，采用MTT法检测不同浓度的蛇毒蛋白提取物对K562细胞生长的影响；应用电子显微镜观察药物作用后K562细胞形态的改变；流式细胞术检测蛋白提取物对K562细胞细胞凋亡作用；Western blot检测细胞增殖酶活性的变化。

OBJECTIVE: Three sesquiterpene lactones 1-O-acetylbritannilactone, isoalantolactone and britannilactone were isolated from the roots of Inula helenium and the flowers of Inula japonica. The antitumor activities of the three sesquiterpenoids in the cell lines of HeLa, HEC-1, SHIN3, HOC-21 and HAC-2 were measured, and the relationship between structure and activity and the possible mechanisms were explored.

目的： 观察菊科植物中分离出的3种倍半萜化合物异土木香内酯、1-氧-乙酰大花旋覆花内酯和大花旋覆花内酯对体外培养的人子宫颈癌HeLa细胞、人子宫内膜癌HEC-1细胞、人卵巢透明细胞癌SHIN3及HOC-21细胞和人卵巢囊腺癌HAC-2细胞增殖的影响，研究这3种倍半萜化合物的体外抑制肿瘤细胞增殖作用及其结构与活性之间的构效关系，进而探讨3种倍半萜化合物的体外抑制肿瘤细胞增殖的可能机制。

Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P ＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

NGF had obvious cytotoxicity on these cell lines ,which shows to be dose-dependent, especially for MGC-803 and BEL-7404 ,their IC_(50) value at the 24~ hours are 49.9mg/L and 55.46mg/L ; Main features of apoptosis under fluorescent microscope include reduction in volume,nuclear chromatin condensation, intensification under jacinth fluorescence; The early apoptosis rate of pristine cells which detected by FCM increases with NGF dose increasing.

2NGF对4种人肿瘤细胞均有细胞毒性作用，且呈剂量依赖关系，尤其是对MGC-803和BEL-7404的抑制作用更强，作用24h的IC50值分别为49.9mg/L和55.46mg/L，荧光显微镜下可观察到MGC-803凋亡细胞的主要特点为：细胞体积缩小，染色质固缩，橘红色荧光增强；流式细胞仪检测到MGC-803细胞的早期凋亡率随NGF浓度的增高而增加，各浓度组与对照组的早期细胞凋亡率比较有明显差异(P＜0.01)。

We also have observed some organs pathological changes of juvenile Jian Carp, such as pancreas acinous gland and zymogen granules of acinous gland cell disappearing, intercellular substance hyperplasia and inflammatory cells soakage, hepatatrophia, liver cell granular or vacuolar degeneration and necrosis, karyolysis or pyknosis, glycogen granules decreasing, metanephros atrophy, metanephric canaliculus epithelium granular or vacuolar degeneration and necrosis, mitochondrion swelling and mitochondrion cristae disappearing, karyolysis, distal convolutal tubule microvilli desquamating, spleen marrow cell degeneration and necrosis, intercellular substance of spleen hyperplasia, spleen atresia, blood corpuscle disappearing.

后肾土黄色、淡褐色或苍白色，肾小管上皮细胞肿胀、颗粒或水泡变性、坏死，细胞内有大量血细胞流出，线粒体肿胀，嵴结构消失，细胞核溶解，肾间质甲状腺滤泡和拟淋巴细胞增生，远曲小管微绒毛脱落、管道细胞界限不清。心脏肌纤维肿胀、颗粒或空泡变性，严重的肌纤维溶解、变细或断裂，肌纤维间水肿、炎性细胞浸润，部分心肌细胞核浓缩。脾脏暗褐色，脾髓质细胞变性、坏死，拟淋巴细胞明显减少，淋巴细胞岛少见、岛中细胞成份减少，黑素巨噬细胞中心减少、体积缩小，脾脏网状基质水肿，脾窦闭锁，血细胞减少。

In order to investigate the theory and method in researching mechanical properties of hepatocellullar carcinoma cells with micropipette aspiration , hepatocellullar carcinoma cells.

细胞的力学特性与细胞的结构和功能密切相关，细胞粘弹性是细胞的一种本质属性，也是细胞力学特性研究的重要内容，微吸管法是一种在单个细胞水平上研究细胞力学特性的先进手段。

To begin with, the adhesive experimental model between SMMC-7721 cells and HUVECs was established in this paper, then expression of integrin β〓 in SMMC-7721 cells was determined by flow cytometer, finally, adhesive mechanical properties of different cell cycle SMMC-7721 cells to collagen Ⅳ were studied by micropipette aspiration technique.

本文首先建立了肝癌细胞与内皮细胞粘附力分析的实验模型，接着应用流式细胞技术检测肝癌细胞上integrin β〓的表达，最后研究了不同细胞周期肝癌细胞与内皮细胞的粘附力学特性。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。