细胞

Result: The nm-HAP-sol present the dispersed and uniformity needle. A431cells inhibition ratio was maximizing at the 5 day in the 400mg/L nm-HAP-sol group, the 200mg/L nm-HAP-sol group and the 100mg/L nm-HAP-sol group, inhibition ratio of the 400mg/L nm-HAP-sol group, the positive control group and the 50mg/L nm-HAP-sol group was respectively (77.89±6.29)%,(77.46±8.26)% and (1.23±0.15)%. It was detected that the number of A431 cells were persistently increasing in the 50mg/L nm-HAP-sol groups while decreasing in the 400mg/L nm-HAP-sol group, the positive control group and abidingly increasing for 4 and 3 days then decreasing in the 100mg/L and 200mg/L nm-HAP-sol group by inverted microscope.

结果：超声乳化法配制的HAP纳米溶胶呈均匀分散的针状；400mg/L、200mg/L和100mg/L溶胶组对A431细胞抑制率在第5天达到各组最大值，此时400mg/L溶胶组与阳性对照组的抑制率分别为（77.89±6.29）%和（77.46±8.26）%， 50mg/L溶胶组抑制率为（1.23±0.15）%；倒置显微镜下见50mg/L溶胶组细胞数目持续增加，聚集成簇，细胞呈现多角形或梭形，胞浆透亮，而100mg/L 和200mg/L溶胶组的细胞数目分别增加至第4天和第3天开始下降，400mg/L溶胶组和阳性对照组细胞数目持续减少，细胞体积缩小，漂浮细胞增多。

There are two types of cells: the acidophile and the basophile. Prolactin cell and adrenocorticotopic hormone cell form the anterior lobe, the gonadotropic hormone cell, growth hormone cell and thyrotropic hormone cell form the intermediate lobe, the posterior lobe consists of melanotropic stimulating hormone cell.

含有嗜酸性和嗜碱性细胞，促肾上腺皮质激素细胞和催乳激素细胞组成腺垂体前叶，促性腺激素分泌细胞、促生长激素分泌细胞、促甲状腺激素分泌细胞组成腺垂体间叶，腺垂体后叶由促黑色素激素细胞构成。

Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示：脾脏PCR检测结果表明1、2两实验组均有阳性病例出现，而对照组全部为阴性，说明实验鸡体内已有ALV-J内蒙株感染；接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化：肝脏、脾脏有程度不同的肿大，镜下观察，骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生，随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶，而对照组没有出现明显病变；免疫状态方面：以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强，ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

Those proteins are the cell special proteins of neuron, glia, photoreceptor, bipolar cell, amacrine cell, horizontal cell and the ganglion cell of retina respectively.

并且可表达视网膜的终末分化细胞的标志，包括：感光细胞标志Rhodopsin、双极细胞标志PKC、无长突细胞标志Syntaxin、水平细胞和无长突细胞标志Calbindin和视网膜节细胞标志Thy-1。

RESULTS: Wound healing rate, wound healing time, histopathology analysis, quantity assay of macrophage, determination of hydroxyproline, proliferation of cell, assay of DNA contents and circle of cells, level of transforming growth factor-alpha, levels of interleukin-1, interleukin-6 and tumor necrosis factor, assay of keratinocyte collagenase-1, level of fibroblast growth factor receptor-1, level of monocyte chemoattractant protein-1 and level of keratinocyte plasminogen activator inhabitor type 2 were selected as the evaluation criteria of wound healing.

结果 筛选了创面愈合率、创面愈合时间、组织病理学分析、巨噬细胞定量分析、羟脯氨酸含量测定、细胞增殖情况、细胞DNA含量和细胞周期分析、转化生长因子-α水平、白细胞介素-1、白细胞介素-6和肿瘤坏死因子水平、角质细胞胶原酶-1含量测定、成纤维细胞生长因子受体-1水平、单核细胞化学诱导蛋白-1水平和角质细胞纤溶酶原活化抑制剂-2水平等十三种创面愈合评价指标。

Interleuk in 2 (IL-2) is an important cytokine released from activated T lymphocytes, which activated lymphokine actived killer cells and natural killer cells , and promoted cytokine production of helper T Cells and natural killer cells , and stimulated T cell growth in vitro and so on . It plays a important role in body immune regulator.

白细胞介素2主要是由激活的T淋巴细胞产生的一类重要的细胞因子，具有激活淋巴因子活化的杀伤细胞和自然杀伤细胞，刺激T辅助细胞和自然杀伤细胞产生细胞因子和促进细胞因子的繁殖以及刺激T细胞在体外生长等功能，因此它是在免疫调节中有重要作用。

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

结果显示：(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散，但猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系，卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准，只有排出第一极体才能作为猪卵母细胞核成熟的标志；(2)在猪COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率，而且可加强卵丘细胞的扩散；(3)在现有实验条件下，在mTCM和mNCSU中添加10％pFF与在mNCSU中添加10％FCS相比可获得较高的猪卵母细胞核成熟率；(4)在不同的成熟液中添加不同的蛋白质对猪卵母细胞核成熟率的影响效果不一样，但对体外受精后的早期胚胎发育影响不明显；(5)成熟液种类对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。

The laboratory research and chinical trials to prove.courage lcecontains active material that it can quickly penetrate through body's cell.so to directly provides for the cell,fill the skin intercellular space the crevice which is senile withered because of the cell stays benind supply the active elements necessary for the body cells metabolism,to activate the function of endothelium cells of blood vessels,promote the blood vessels balance, compose and secrete various blood vessels active elements,contains abundantsulfuric cartilage element,sulfuric amido etc.,all of fatty acid in human body.and it can accelerete the re-generation of blood cells,add the quantity of the hemoglobin and red blood corpuscle so to improve the blood parameter,and fundamentally improve the body cells metabolism and nutrition supply for all the organs,as a result,you will thoroughly refresh yourself and bloom vitality of youth.

实验室研究和临床试验证明，LCE所含活性物质能够迅速穿透人体细胞，直接为细胞供养，填补肌肤细胞间因细胞衰老干瘪而留下的空隙全面补充人体细胞新陈代谢所必须的活性物质，激活血管内皮细胞功能，促进血管平衡，合成和分泌多种血管活性物质：富含的硫酸软骨素、硫酸氨基葡萄糖等能改善和调节人体脂代谢，有效清除体内血液中多余的脂质和脂蛋白清除在血管壁内膜沉积的脂类物质，保持人体脂肪酸均衡：能够加快血液细胞更新，提高血液中的血红蛋白和红血球数量，改善血液参量，从根本上改善人体细胞的新陈代谢，改善人体各组织器官的营养供给，使人由内而外脱胎换骨，重新焕发青春活力。

The laboratory research and chinical trials to prove.courage lcecontains active material that it can quickly penetrate through body's cell.so to directly provides for the cell,fill the skin intercellular space the crevice which is senile withered because of the cell stays benind supply the active elements necessary for the body cells metabolism,to activate the function of endothelium cells of blood vessels,promote the blood vessels balance, compose and secrete various blood vessels active elements,contains abundantsulfuric cartilage element,sulfuric amido etc.,all of fatty acid in human body.and it can accelerete the re-generation of blood cells,add the quantity of the hemoglobin and red blood corpuscle so to improve the blood parameter,and fundamentally improve the body cells metabolism and nutrition supply for all the organs,as a result,you will thoroughly refresh yourself and bloom vitality of youth.

实验室研究和临床试验证明，LCE所含活性物质能够迅速穿透人体细胞，直接为细胞供养，填补肌肤细胞间因细胞衰老干瘪而留下的空隙全面补充人体细胞新陈代谢所必须的活性物质，激活血管内皮细胞功能，促进血管平衡，合成和分泌多种血管活性物质：富含的硫酸软骨素、硫酸氨基葡萄糖等能改善和调节人体脂代谢，有效清除体内血液中多余的脂质和脂蛋白清除在血管壁内膜沉积的脂类物质，保持人体脂肪酸均衡：能够加快血液细胞更新，提高血液中的血红蛋白和红血球数量，改善血液参量，从根本上改善人体细胞的新陈代谢，改善人体各组织***的营养供给，使人由内而外脱胎换骨，重新焕发青春活力。

PCI2 cells were treated under glucose deprivation as model group. Method Morphological and cytoflowmetric methods (the mitochondrial transmembrane potential; MEF method) were applied to evaluate the protective effect of SAB. The RNA levels of grp75 were measured by RT-PCR and immunocytochemistry were performed to investigate the expression level of PC12 cells under glucose deprivation and treated by SAB.

以PCl2细胞（大鼠肾上腺嗜铬细胞瘤细胞）的无糖培养建立细胞缺糖损伤模型，MTT法检测细胞的存活率；应用流式细胞仪检测亚二倍体细胞的比率及线粒体跨膜电位的改变；应用免疫细胞化学、RT-PCR法研究细胞内应激蛋白grp75的表达变化，激光共聚焦显微镜观察SAB作用下Ca(上标 2+)的变化。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。