细胞

Contents include cell culture 、 cell fusion 、 Cytochemistry 、 apoptosis detection 、 protein expression and function determination , etc.

课程涉及的主要实验内容包括细胞培养、细胞融合、细胞化学、细胞凋亡检测和蛋白质的表达与功能检测等。

Cells were characterized by immunohistochemistry, cytochemistry, FACS and ultrastructure to identify and detect the differentiated population and markers.

通过免疫细胞化学、细胞化学、流式细胞分析法及透射电镜对其分化后细胞进行鉴定。

As the development of monoclonal antibody and quantitated fluorescent cytochemistry technique, FCM has been used more extensively in biology, clinical medicine, pharmacology, material and so on.

本文就流式细胞术的发展历史、流式细胞仪的原理及在各领域的应用进行综述，阐明现代流式细胞术由于结合单克隆抗体技术、定量荧光细胞化学技术，使其在生物学、临床医学、药物学、材料学等众多研究领域中的应用有更加突飞猛进的发展。

Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞，使之转化为成骨细胞；用RT-PCR检验成骨细胞；不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基，作用于成骨细胞，并分析生长曲线，分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus.

皮层在发育早期以及纹状体和海马CA区在发育各期检测到极少数为新生神经元细胞，多数分化为胶质细胞；海马齿状回以神经元细胞再生为主；胶质细胞的再生随脑组织发育的成熟而逐渐减少。

Methods Morphology observation, chromosome cytogenetics, tumorigenesis in nude mice, and the sensitivity to virus were examined.

采用细胞形态学观察、细胞遗传学染色体分析、细胞在裸鼠体内恶性生长情况的组织病理学检测以及细胞对病毒的敏感性等方法。

The positive expression of Slit2 located in the cytoplast on oligodendrocyte and astrocyte.The positive cells were found at 3d after spinal cord injury, reached peak on 7d after injury, declining after 14d. The change of Slit2 was correlative with the rehabilitation and regeneration of the axon on the forepart period.

免疫组织化学检测：Slit2蛋白定位于星型胶质细胞和少突胶质细胞的胞浆，呈棕黄色染色，于脊髓损伤后第3 d出现，阳性细胞逐渐增多，于脊髓损伤后第7 d，Slit2阳性表达的细胞数达到高峰并趋于稳定，伤后第14 d开始逐渐下降，呈一过性增高。

Numbers of secretory granules, oplasmic reticulums, mitochondrias in ACTH cells all changed distinctly. GTH cytoplast were full of big and small, high density electron secretory granules.STH cells were in activity synthesis and secretion, secretory granules were from fusion to release. TSH cells cytoplast evacuated secretory granules, electron density decreased and vacuofes appeared.

ACTH细胞分泌物颗粒数量、内质网及线粒体均发生明显变化；GTH细胞胞质中充满电子密度较高的大、小两种分泌颗粒；STH细胞处于活跃的合成和分泌状态，分泌颗粒呈现出由融合到释放的状态；TSH细胞胞质中分泌颗粒排空，且电子密度降低，出现大空泡。

The DAK had obviously killing effects on lung cancer cell CALU-6 extracted from TCA.

树突状细胞活化的杀伤细胞对于肿瘤细胞全抗原来源的肺癌细胞系CALU-6肿瘤细胞有明显的特异性杀伤作用。

And the decreasing of renal function was consistented with renal pathological changes.

蛋白的表达及细胞内钙含量随时间延长进行性增加，通过激活促细胞凋亡的程序而诱导肾脏细胞凋亡，与肾脏结构受损、功能下降是一致的。关键词：脑缺血；'肾脏；细胞凋亡；TNF一a；MDA；CaZ

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。