细小病毒

The purpose of this research work is to establish the method involved in the molecular biology and the serology to detect the PPV in the related biological products.

本论文旨在建立猪细小病毒的分子生物学及血清学检测方法，并对相关生物材料进行检测。

Canine Parvovirns VP2 gene was solubly expressed in E. coli.

犬细小病毒VP2基因在大肠杆菌中的获得了高效可溶性表达。

Objective: To solubly express the canine parvovirus VP2 gene efficiently in Bacillus coli, and lay a foundation for the preparation of monoclonal antibodies against VP2 protein.

目的：犬细小病毒VP2基因在大肠杆菌中高效可溶性表达，进而为其特异单克隆抗体的制备奠定基础。

If you test for it in your pig herd it is almost certain it will be present.

如果你检查你的猪群中是否存在猪细小病毒，几乎可以肯定都是阳性。

Infection itself causes no clinical symptoms other than the presence of mummified pigs at farrowing.

猪细小病毒感染对感染的猪只并不造成任何临床症状，只是在分娩时产木乃伊胎。

If the foetus is infected at less than 35 days of age, before there has been an opportunity for bone development, death results, followed by complete absorption and ultimately a small litter is born.

如果胚胎在35天之前感染猪细小病毒会发生胚胎死亡，由于此时胎儿的骨骼尚未发育完全，死胎随后将被完全吸收。结果会导致产仔数减少。

Methods: Protein analysis software-PROSPECT was used to divide VP2 into different sections according to hydrophile and antigen character of VP2 amino acids. Correspondent down-lead was designed to extend the section.

以犬细小病毒VP2基因重组质粒为模板，通过蛋白质分析软件PROSPECT分析，根据VP2基因所编码的氨基酸的亲水性和抗原性，把VP2基因分成不同区段，设计相应引物并扩增出相应片段。

These new types have replaced the original CPV type 2 worldwide, and CPV type 2b predominates overwhelmingly relative to CPV type 2a and is endemic in most populations of domestic canids in China.

通过单克隆抗体识别、限制性酶切及PCR等方法进行的流行病学调研表明：自从最早期的CPV-2型细小病毒出现以来，CPV已演化出新抗原型，即CPV-2a与CPV-2b型。

The isolated virions were proved to have morphological features of porcine parvovirus observe...

此外，对从现场采集的有死产或不孕史的母猪血清23份做血清学检查，其抗猪细小病毒阳性检出率高达91.3%。

Goose Provirus is the pathogen of highly contagious and fatal infectious diseases of goslings and Muscovy ducklings.

鹅细小病毒(Goose Provirus，GPV)可引起雏鹅、雏番鸭高度致死性传染病—小鹅瘟。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。