细小病毒

Porcine parvovirus is a major cause of reproductive failure in swine, manifested as embryonic resorption, fetal mummification, abortion and stillbirths .The virus is ubiquitous among swine throughout the world and is enzootic in most herds that have been tested.

猪细小病毒(porcine parvovirus，PPV)为引起母猪繁殖障碍的主要病原之一，猪细小病毒感染的主要特征是孕猪在怀孕前容易受到感染，可引起胚胎或胎儿的感染和死亡，导致母猪发生流产、死胎、胎儿木乃伊化及新生仔猪的死亡。

Key words: Muscovy duck parvovirus;goose parvovirus;PCR;differential diagnosis

番鸭细小病毒；鹅细小病毒；聚合酶链式反应；鉴别诊断

In order to diagnose disease caused by muscovy duck parvovirus rapidly and accurately, and at same time, further study the taxonomic relationship between muscovy duck parivovirus and goose parvovirus, two pairs of PCR primers (LHMP1/LHMP2 and LHGP1/LHGP2) were designed respectively base on complete nucleotide sequences of Muscovy duck and Goose parvoviruses registered in the GenBank, The LHMP1 for the upper one and the LHMP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in MDPV, and the LHGP1 for the the upper one and the LHGP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in GPV.

为了能快速、准确地对番鸭细小病毒病进行诊断，同时为了能进一步弄清番鸭细小病毒与鹅细小病毒在分类学上是一种怎样的关系，比较GenBank中MDPV和GPV的基因序列，针对这两种病毒左阅读框编码非结构蛋白基因序列的相同区段，分别构建两对PCR引物。第一对引物LHMP1／LHMP2是根据MDPV的基因序列设计的，上游引物针对MDPV基因序列的454～472；下游引物针对基因序列的1173～1155，为19个碱基，扩增跨幅为719bp。

To establish a method to differentiate Muscovy duck parvovirus from goose parvovirus, primers GPVF/R1 and MDPVF1/R1 were designed according to the genomic sequences of GPV and MDPV to amplify the virus culture and nucleotide of GPV, MDPV, duck plague virus, duck hepatitis virus, duck reovirus, canine parvovirus and feline panleucopenia virus.

根据番鸭细小病毒和鹅细小病毒基因组的非同源序列各设计了1对引物MDPVF1/R1和GPVF1/R1，建立了一种PCR方法，用该PCR方法分别对GPV、MDPV、鸭瘟病毒、鸭肝炎病毒、鸭呼肠孤病毒、犬细小病毒和猫泛白细胞减少症病毒的病毒培养物及其核酸进行扩增。

Porcine parvovirus and pseudorabies virus are the main causes of infectious reproductive failure in swine.

猪细小病毒和伪狂犬病毒均是引起母猪繁殖障碍的主要病原，猪细小病毒的危害主要表现为，导致初产母猪或血清学阴性的经产母猪发生繁殖障碍，其它年。。。

For evaluating the vaccinal effect,This indirect ELISA was used to detect sera collected from 160 dogs; after one year, 60dogs among these dogs was also collected sera for detection in this indirect ELISA.

为了评估犬细小病毒的免疫情况，将建立的间接ELISA方法应用于140份犬细小病毒血清抗体的检测，而且一年后，对其中的60只犬再次采集血清进行检测。

The biological characteristic of many vertebrate parvoviruses have been clarified, for example the genome organization, the structure of capsids protein of virus particle, and the relationship between the structure and its function.

它与其它细小病毒一样，病毒粒子无包膜，呈球状二十面体对称，直径22nm，基因组为单链线状DNA分子，含有五种结构蛋白，嶂螂浓核病毒基因组全长为5454个核昔酸，于C含量为38.13%。

The materials including small intestines,large intestines,heart,lymph node,spleen,lungs,liver,kidneys,etc.obtained from a baby dog that were diagnosed to be canine parvovirus in the lab,were studied by freezing section and HE staining.

犬细小病毒病，又称犬细小病毒性肠炎，是由犬细小病毒(Canine parvovirus，CPV)引起的一种高度接触性传染病[1]，临床上以剧烈呕吐、排血样粪便和心肌炎为特征[2]。

Canine parvovirus is a very hardy virus, and this is one of the reasons that parvovirus is so dangerous.

犬细小病毒是一个非常坚强的病毒，这是一个原因，细小病毒非常危险。

A lot of viruses can induce cell cycle arrest, for example paryoviruses, herpesviruses, retroviruses et al.

许多病毒的感染会导致宿主细胞周期的阻滞，如细小病毒、疱疹病毒、逆转录病毒等等。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。