组织蛋白酶

Methods This study consisted of 36 male Wistar rats. A rat model of intestinal adhesions was done as follows: serous membrane of the caecum was scuffed, its raw surface was dropped with absolute alcohol, the mesentric artery of the caecum was then clamped. The rats were divided into three groups. PSS, sodium hyaluronate or normal saline was applied to the scraped cecum before closing the abdomen.

选用雌性Wistar大鼠36只，刮伤其盲肠浆膜，创面滴无水乙醇，钳夹盲肠系膜动脉制造大鼠肠粘连的动物模型，随机将动物分成3组，分别在关腹前于创面涂布藻酸双酯钠、透明质酸钠、生理盐水，术后14 d将大鼠处死，肉眼观察肠粘连程度并对粘连组织的基质金属蛋白酶2和金属蛋白酶组织抑制物2水平进行检测。

Intimal areas were measured using morphometric analysis of perfusion-fixed vein graft specimens, and intimal thickness was calculated using circumferential measurements. The SMC proliferation was studied by the immunohistochemical detection of proliferating cell nuclear antigen. Expression of MMP-2、MMP-9 mRNA in vein grafts and unoperated control Vein grafts was detected by reverse transcription polymerase chain reaction. Substrate gel zymography was used to determine the proteolytic activity.

分别行HE染色、Verhoeff弹性纤维染色观察组织病理变化，计算机病理图象分析系统测量新生内膜厚度及面积，免疫组织化学方法检测静脉壁增殖细胞核抗原表达以观察细胞增殖情况，半定量逆转录-聚合酶链反应检测静脉壁基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)mRNA的表达，明胶酶谱法检测MMP-2、MMP-9活性，比较各组之间的差别。

This process is the result of the imbalance between matrix metalloproteinases zymogen activations and interaction of the active forms with specific tissue inhibitors of metalloproteinases.

本实验以大鼠为模型旨在研究急性胰腺炎中基质金属蛋白酶-2(MMP-2)与基质金属蛋白酶特异组织抑制物-2(TIMP-2)在胰腺组织中的表达，探讨其与急性胰腺炎时器官损伤程度的相关性。

Objective: To investigate the effects of Hovenia dulcis extract on mRNA expression of MMP-13 and TIMP-1 mRNA in hepatic tissue in experimental rats.

中文摘要：目的：探讨枳子提取物对实验大鼠肝组织基质金属蛋白酶-13(MMP-13)与金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响。

Objective To explore the significance of expression of matrix metalloproteinases and tissue inhibitor of metalloproteinases -2(TIMP-2) in benign and malignant hydatidiform molr.

目的 探讨基质金属蛋白酶(MMP- 2 )及金属蛋白酶组织抑制因子(TIMP- 2 )在良、恶性葡萄胎组织中的表达及临床意义。

We study the express of heparinase,matrix metalloproteinase MMP-2,MMP-14 and cadherin in the gastric cancer tissue and perigastric normal mucosal tissue,and make a statistics analysis between heparinase, matrix metalloproteinase MMP-2,MMP-14,E-cadherin and gastric cancer biological behavior.We hope to knom the possibility that heparinase,matrix metalloproteinase MMP-2,MMP-14,E-cadherin are regared as clinical screen index of the gastric cancer recrudescence or metastasis.

本文就肝素酶、基质金属蛋白酶MMP-2、MMP-14、钙粘蛋白在胃癌组织及胃周正常粘膜的表达进行了研究，并就肝素酶、基质金属蛋白酶MMP-2、MMP-14、钙粘蛋白与胃癌的生物学行为进行了统计学分析，以期了解肝素酶、基质金属蛋白酶MMP-2、MMP-14、钙粘蛋白作为胃癌复发或转移的临床筛选指标的可能性。

Methods immunohistochememstry(s-p technique) and pathologic image semi-quantification were used to detect the expression of mmp-9 and timp-1 in endometrium of 30 unexplained infertility patients and 20 normal early pregnant deciduas and 20 normal endometrium.

目的 检测基质金属蛋白酶-9(mmp-9)和组织金属蛋白酶抑制物-1(timp-1)在不明原因不孕、正常早孕蜕膜组织和正常子宫内膜中的表达，探讨其与不明原因不孕关系。

Methods Immunohistochemical staining were used to detect the expression and location of calpain in aging rat cochleas, and real time PCR assay were used to detect mRNA level of calpain and calpastatin in cochlear extracts.

采用免疫组织化学染色确定钙蛋白酶在老年大鼠耳蜗中的表达水平和组织定位，采用即时PCR技术分析耳蜗提取物中钙蛋白酶和钙蛋白酶抑素 mRNA水平的变化。

Retinal pigment epithelial cell ; immunohistochemistry ; matrix metalloproteinase-2; tissue inhibitor of matrix metalloproteinase-1; transforming growth factor β_

视网膜色素上皮细胞；免疫组织化学；基质金属蛋白酶-2；基质金属蛋白酶组织抑制剂-1；转化生长因子β1

Objective To determine the distribution and influence of lysosomal neuraminidase, protective protein/cathepsin A and β-galactosidase in the inner ear of the mouse, and to observe their auditory alterations in enzyme deficiency.

目的：研究溶酶体神经氨酸酶(neuraminidase， Neul)、保护蛋白／组织蛋白酶A(protective protein/cathepsin A， PPCA)和β-半乳糖苷酶(β-galactosidase，β-gal)在小鼠内耳的分布及相互影响，观察各自酶缺乏时的听功能改变。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。