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The results are as follows: the dwarf mutant A846 pertain to half-dwarf type, which height is about 38.64 cm on average, preponderating over the half-height of the wild-type, the elongation stage of the mutant represent dwarf character, internode elongation patterns of caulis are similarly the sh-type dwarf mutant, in which the first internode shows specific reduction, the elongation of these cells in the elongation zone immediately above the IM was dissimilar to the wild-type, longitudinal length of parenchyma cells were curtate, also the first internode exhibit markedly, which average is under the corresponding internode of the wild-type, the differentiation of the IM of each internodes of stem was similar to wild-type, as well as the number of internodes and the length of sheath of flag leaf.

结果表明:突变体A846平均株高38.64cm,大于野生型株高的一半,为半矮秆类型;其矮生性在拔节期显著表现;主茎茎秆各节间收缩比例不一,穗下第一节间显著缩短,与sh-型突变体类似;茎秆各节间居间分生组织细胞分化正常,由居间分生组织分化的细胞延伸受到不同程度的阻碍;各节间基本组织细胞纵向长度相应缩短,穗下第一节间缩短比例最大,其平均值小于野生型的一半;主茎节间数目与旗叶的叶鞘长类似于野生型。

This papers explore the correlation between the thickness of blade、cuticula、epidermis cells、fence form、sponge form,the ratio of the fence form and sponge form,the layer of the fence form,the length of cell and the yield,suitable system,resistance of the blade anatomical structure by paraffin section method and observing in optical microscope using tea varieties in Guangxi,such as the No.1 of Guilv, and provide a scientific basis for tea scientific research and production.

本文主要对近年来广西选育成的桂绿1号等新品种品系通过石蜡切片法,并采用光学显微镜观察叶片解剖结构特征,探索叶片解剖结构中的叶片、角质层、表皮细胞、栅栏组织、海绵组织等厚度及栅栏组织与海绵组织厚度的比值、栅栏组织层数、细胞长度等与产量、适制性、抗逆性之间的相关性,为茶叶科研、生产利用提供科学依据。

It was a new mousegene in GenBank and named as SRG-L(spermatogenesis related gene expressed in the latestages of spermatogenic cells). The sequence of this cDNA has been deposited in GenBankwith the Accession No. AY352586. 2. Analyses of expression characteristics of SRG-L during mouse spermatogenesis.The results of RT-PCR and Northern Blotting indicated that no expression signal of thisgene could be observed in primitive type A spermatogonia and type B spermatogonia. Asignal first appeared in diplotene/pachytene spermatocytes, and was significantlyintensified in round spermatids and elongating spermatids, findly went down to beundetectable in mature spermatozoa.

RT-PCR和Northern Blotting分析表明:该基因在早期A型和B型精原细胞中不表达,从双线期初级精母细胞阶段开始表达并逐渐上调,在粗线期初级精母细胞、圆形精子细胞及长形精子细胞中均检测到高水平的mRNA,但在成熟精子中消失,具有明显的阶段特异性表达特征,并且组织特异性表达分析显示该基因在睾丸组织中的呈高表达。

This process aims at recapitulating the normal embryologic development of the organ of interest. The main difference between in vivo technology and in vitro technology is: in vitro technology emphasis on in vitro cell culture. It has special value for the specific tissue such as articular cartilage.

体内组织工程技术与体外组织工程技术的目标相同,都是实现组织完全再生;区别在于其达到再生的方法:体外组织工程技术强调细胞的体外培养,并使这些细胞产生功能,这对有些组织有特殊价值,如软骨。

The leaf epidermis structures of 6 species of Rhododendron had been observed under LM and SEM. The results show that all share together common features as follows: the leaf blades are typical back-abdomen bifacial types, the upper epidermis are formed by two or three layer bigger cells which inside layer cell is bigger than outside one, and the upper epidermis are covered with thicker cuticle.

利用光学显微镜和扫描电子显微镜观察了6种杜鹃属植物的叶片结构,结果表明,它们具有共同形态结构特征:典型的异面叶,上表皮由2~3层细胞组成,内大外小或内、外等大,有较厚的角质膜,无气孔器分布;下表皮均由1层细胞组成,排列紧密;栅栏组织由2层以上长柱形细胞组成,排列紧密;海绵组织细胞较短,排列较疏松,细胞间隙较大;均有表皮附属物。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养,细胞铺满培养瓶底后首次传代,通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化;通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况,对细胞进行鉴定。

Three-dimensional Matrigel constructs contained aleolar forming units comprised of cells displaying AE2 cellular ultrastructure while expressing the SpC gene and gene product. The addition of tissue-specific growth factors induced formation of branching, sacculated epithelial structures reminiscent of the distal lung architecture.

通过对细胞亚微结构的观察和对SpC基因及其表达产物的分析,研究者发现培养体系中肺组织形成单位中具有能向肺泡II型上皮细胞分化的细胞;而通过添加组织特异性的生长因子,他们还发现这一做法会促进培养组织形成具有分支和囊状上皮结构的类似终末肺泡组织的形态结构。

A 46-year-old man presented with a gradually growing nodule on the left dorsal foot, which had been present for over 10 years. In the beginning, the lesion was treated as a keloid. Because of poor response to treatment, biopsy was arranged. Sections showed a dermal tumor composed of numerous foamy cells, hyalinized wiry collagen, spindle cells and entrapped collagen. The foamy cells were positive for KP1(CD68) and negative for S-100 protein and HMB-45. The histological and immunohistochemical results were consistent with the diagnosis of lipidized fibrous histiocytoma.

一位46岁男性从十年前开始於左足背上出现一个逐渐长大之结节,一开始被当作蟹足肿给予治疗,因为对治疗的反应不好,故安排了切片检查,病理显示一个由大量泡沫状细胞,坚硬而透明之胶原纤维,梭状细胞,胶原纤维束所构成的真皮肿瘤,免疫组织化学染色显示KP1(CD68)阳性,S-100 protein与HMB45阴性,综合病理与免疫组织化学染色结果,诊断为脂肪性纤维组织细胞瘤。

CGRP content in myocardium gradually enhanced from normal group to controls group, and model group's was the highest. We found immunopositive neurons and nerve fibers of nNOS and CGRP by immunochemistry in normal myocardium. They were mainly round-shaped and oval-shaped, also tadpole-shaped、 shuttle-shaped、triangle-shaped、ring-shaped and irregular-shaped. Nv of neurons and nerve fibers in atria were more than in ventricle in some degree; Those in right part of heart were more than in left. Morphology of CGRP neurons changed in model group, the number of ring-shaped neurons increasing, while the round-shaped and oval-shaped decreasing, and Nv and Sv of model group were markedly less than normal. Expression of nNOS mRNA was overly higher than normal group, but CGRP was opposite.

心肌组织CGRP的含量由正常组、实验对照组至CHF组逐渐升高,差异显著;免疫组化研究表明心肌组织中存在nNOS和CGRP免疫阳性神经细胞及纤维,细胞形态多样,正常多以圆形和椭圆形为主,还有梭形、蝌蚪形、三角形、指环形及不规则形,细胞密度心房高于心室,右心高于左心;CHF组CGRP神经细胞形态有一定改变,淡染的指环形细胞增多,而深染圆形或椭圆形细胞减少,其面、数密度均明显低于正常组,差异显著;用RT-PCR测得CHF组nNOS mRNA极度表达,显著高于正常组,而CGRP表达明显低于正常组。

Art A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites.

lant Cell and Tissue Culture|植物细胞和组织文化植物细胞和组织文化彻底描述植物细胞文化和遗传学的转变,在全部主要的田地和种植园庄稼上包括详细的章。提出一全部必要实验室技术给开始,营养,维护和植物细胞和组织文化的贮存的综合新闻报导,包括关於这些题目的讨论,包括谷类,豆科植物,菜蔬,马铃薯,其他根和块茎,油菜籽,适度水果,热带水果,种植园庄稼,森林树和ornam

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