组织细胞的

Under the full sun, the fresh weight of Ajuga multiflora and Belamcanda chinense were the heaviest. Compared with full sun, the ratio of leaf thichness of palisade mesophyll and epidermal mesophyll of plant was lower under 80% shading.

与全光照相比，在遮光80％条件下金叶过路黄与多花筋骨草，其叶片的栅栏组织与海绵组织细胞差异较大，表现为栅栏组织宽度减小，海绵组织细胞之间间隙加大。

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活，ICAM-1表达升高，与被激活的PMN表面表达的LFA-1相结合，"PMN-EC"相互作用加剧，一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍；另一方面粘附的PMN过度吞噬或激活，当白细胞吞噬的颗粒不能被封闭隔离，连同细胞内的酶被释放出来，其中的PMN-elastase能够降解细胞基质中各种成分，水解多种蛋白，加重胰腺的毛细血管内皮细胞和腺泡的损伤；释放的溶酶体酶使组织蛋白水解，产生的不饱和脂肪酸引发脂质过氧化方应，破坏细胞膜的结构和功能；释放的炎性细胞因子，激发其他的免疫细胞的功能，导致进一步的组织损伤和坏死，加重SAP的病理损伤，最终导致休克、脓毒血症及多器官功能障碍等严重后果。

Cumulative data have been documented that mesenchymal stem cells can differentiate into osteoblasts, chondrocytes, adipocytes, tenocytes, myotubes, neural-like cells, hematopoietic-supporting stroma and other non-hematopoietic cells derived from mesoblast or ectoblast.

大量研究表明，体外培养的骨髓间质干细胞在合适的诱导条件下，可分化为成骨细胞、成软骨细胞、脂肪细胞、成肌细胞、成腱细胞和神经元样细胞等多种非造血组织特别是中胚层和神经外胚层来源的组织细胞。

Met hod: Hypertrophy of prostate model rat was established by injecting t estosterone to gelding male rats. After being treated with Qianlie Huichun ig 30 days, the rats were killed and prostate glands were resected for examination. The Fas expressio n was examined by immunobistochemical SABC.

采用大鼠去势后注射丙酸睾酮引起前列腺增生法造模，灌胃给药 30d后，处死大鼠取前列腺组织并称湿重，用免疫组化方法检测前列腺组织中Fas阳性细胞的表达率，用流式细胞术检测细胞凋亡率和观察细胞凋亡峰。

Healthy spikes of high-resistant varieties had thicker cell wall and tissue of cortical sclerenchyma, more number of cortical sclerenchyma layers, more number of fibrovascular bundles, smaller area of green subcutaneous tissue in rachis, and theses differences, except number of fibrovascular bundles, enlarged over time. Considering inoculated spikes, cell wall and tissue layers of cortical sclerenchyma displayed different, and to how much extent the relationship between this difference and anti-extension ability of variety had not determined. 6. On the analysis of agronomical characters and molecular marking technology, more than 30 alien anti-Gibberella wheat materials and two mutants coming from this experiment were evaluated their hereditary multiplicity.

实验结果还证明，高抗品种和感病品种的穗轴组织结构确实存在一定差异，在健康穗中，主要表现在高抗品种的皮层厚壁细胞壁和皮层厚壁组织较厚，皮层厚壁细胞层数较多，维管束数目较多，穗轴表皮下绿色组织面积较小，抗、感品种间的差异达显著水平，随发育时间延长，高抗品种的皮层厚壁细胞壁厚度、厚壁组织厚度和厚壁细胞层数增加的幅度较大；在病穗中，主要表现在高抗品种的皮层厚壁组织细胞层数和厚壁细胞壁厚度增加的幅度较大。

RESULTS摘要: Microscopically, the lesion consisted of abundant collagen fibrils, proliferated histiocytes and numerous infiltrated plasmocytes, lymphocytes and eosinophilic granulocytes. Fusiform or polygonal histiocytes had abundant, granular, eosinophilic cytoplasm or clear cytoplasm containing radially oriented strands or resembled xanthoma cells. Emperipolesis of numerous small lymphocytes was seen in the cytoplasm of some large histiocytes.

结果摘要：镜下可见丰富的胶原纤维和组织细胞增生以及大量浆细胞、淋巴细胞和嗜酸粒细胞浸润，组织细胞呈梭形、多边形，胞质嗜酸性颗粒状、放射条纹状或泡沫状，一些大组织细胞质内有多量小淋巴细胞。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Histologically,the activity of cell division in scutellar epithelium and callus cluster derived from it was higher in cultivar Aikoku than Moritawase.Moreover different types of differentiation in the epithelial callus were observed,the rootlike structures and globular cell clusters with epidermislike structure were found in the calli of cultivar Moritawase and Aikoku respectively.

组织学的观察表明，Aikoku的种胚盾片上皮组织以及由上皮细胞起源的愈伤组织的细胞分裂活性高于Moritawase；而且在上述2个品种中还观察到不同的细胞分化类型，在Aikoku的愈伤组织中观察到了带有表皮状结构的圆形细胞块，而在Moritawase的愈伤组织中却观察到了根状结构。

Some exudation and bleedings happen in the pulmonary alveoli, some inflammatory cells infiltrate into lung tissues; in the serum AMY, ALP, TNF-α, IL-6 increases obviously high, COX-2 expression increase; The expression of NF-κB in big rat lung of SAP increase, and the nucleus is thick dyed, it explain that the NF-κ moved into the nucleus; Stimulate the inflamatory reaction.

我们的实验中见到SAP组的大鼠肺脏组织细胞腔扩大、肺泡壁断裂，有的出现肺大泡，肺泡内伴有渗出及少量非出血，肺组织可见咽细胞浸润；血清中AMY、ALP、TNF-α、IL-6明显增高；在SAP组大鼠肝细胞、肾小管上皮细胞、肺脏小气管及肺泡上皮上COX-2表达增加；NF-κB在SAP大鼠肺脏中表达增加，并且出现核浓染，说明NF-κB前移入核；启动炎症反应等一系列反应SAP大鼠受损伤的肺组织中AQP-1无论在mRNA水平上、蛋白水平上还是免疫组化染色病理学检测上都明显下降。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性，通过建立猪皮肤成纤维细胞系，将所建细胞系与人胚胎肾293细胞体外共培养，并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验，结果表明，猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中，猪内源性逆转录病霉感染人胚胎肾细胞，进一步证实和拓宽了猪细胞PERV感染人细胞的范畴；猪皮肤成纤维细胞移植SCID鼠皮下后，导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官，但是并未检测出SCID鼠组织中表达PERV env RNA。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。