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With the development of cardiac tissue engineering, it is feasible to repair the injured cardiac muscle by the cell transplantation vivo.

随着心肌组织工程学的发展,通过细胞体内移植的方法而进行的细胞心肌成形术,为病损的心肌提供了修复的可能性。

The changes of MDA in the plasma, CINC/IL-8 and NO in the serum, MPO in the skeletal muscle were studied at the end of ischemia and 1h, 2h, 4h, 8h, 12h during reperfusion.

分别于缺血4h末,再灌注1、2、4、8、12h,测定各组血浆中的MDA、骨骼肌组织中的MPO,血清嗜中性粒细胞化学趋化因子(CINC/IL-8),血清一氧化氮,用流式细胞仪及免疫组化法观察各组白细胞上粘附分子CD11b/CD18和骨骼肌血管中ICAM-1的表达情况及各组的组织学改变。

To solve this problem, we consider that use of tissue-specific regulative elements in construction of plasmid vector for molecular conjugate vector may limit the expression of foreign gene to targeting cells further.

为解决这一问题,我们考虑在构建分子偶联载体中的重组质粒表达载体时,应用组织细胞特异性调控序列,以期在转录水平进一步限制外源基因的表达于靶细胞中,从而保证受体介导基因转移的靶向性,同时可望导致外源基因的高效表达。

Methods] A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法] 采用基因芯片技术,按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA;逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针,在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

Dialdehyde starch not only improves the mechanism function of PLA,but also has good effect on cells.And it doesn'〖KG-3〗t affect the growth and metabolism of cells.So it is feasible as scaffold of tissue engineering artificial skin.

双醛淀粉作为良好的增柔剂在改善聚乳酸网架机械性能的同时,也使其具有良好的细胞相容性,不影响细胞的生长增殖和代谢,可以进一步用作组织工程皮肤的支架材料。

Moreover, the production of mucin and infiltration of inflammatory cells in the lungs of OVA-sensitized and challenged mice were not affected by DIO treatment.

在肺脏组织中的IL-4 mRNA的表现、病理组织切片炎症细胞浸润情形及黏液的生成也都显示DIO不具有降低呼吸道局部炎症反应的作用。

Our experiment is unique to clone porcine IL-4 gene from lymphocytes of the local species of Chenghua porcine and systematically study its expressing effect in vivo on the immune system and immune responses of mouse and pig to trivalent vaccines against Swine fever, the Pasteurellosis and Erysipelas suis and pig paratyphoid vaccine.

将得到的序列在GenBank和EMBL数据库中进行了同源比较,结果表明它与来源于长白猪肠粘膜固有层淋巴细胞的白细胞介素-4有99%同源,与来源于杂交猪T细胞的白介素-4有96%同源,说明猪种及组织细胞来源的不同可能使基因有一定的差异。

The neural stem cells can be isolated from human embryonic cerebral tissue . The ratio of cell clone formation from smaller gestational age is higher than that from elder gestational age .The differentiation of neural stem cells from hindbrain is easier than that from forebrain.

从人胚脑组织的前、后脑中分离出神经干细胞;较小胚龄的脑组织原代培养时细胞克隆的形成比例较高;后脑组织分离的南京医科大学硕士学位论文神经干细胞比前脑组织来源的更容易分化。

Its aim is to determine how tissues are organized at all structural levels, from cells and intercellular substances to organs.

它的目的是要确定从细胞、细胞间的物质到各个器官的所有结构层次是如何组织起来的。

Methods Immunohistochemical SP method was used to detected c-erbB-2 expression,lymph node metastasis and karyokinesis of cancer cells in breast infiltrating ductal carcinoma.

结果c-erbB-2阳性表达主要分布在肿瘤细胞的胞膜上;随着c-erbB-2表达强度的降低,肿瘤淋巴结转移率呈减少的趋势;肿瘤组织中细胞的核分裂数目随着c-erbB-2的表达强度增加而增多。

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