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Results: The numbers of Bombesin positive pulmonary cells, the lamina propria S-l00 protein, neuron-specific enolase positive nerve fibers, IgE positive cells, mast cells and IgE positive mast cells significantly increased in bronchiectasis. The changes of pulmonary endocrine cells, nerve fibers and IgE positive cells were more significantly in hyperplastic BALT areas. The S-100 and NSE were found in lymphoid tissue and BALT. A close contact was found between mast cells and the S-100 positive nerve fibers. An IgE positive outer zone was found on MC surface. Mast cells and IgE positive cells were seen in the bronchial epithelium and alveolar septa.

结果:支气管扩张症中,支气管上皮蛙皮素阳性细胞、固有膜S-100蛋白和神经特异性烯醇化酶阳性神经纤维、IgE阳性细胞、MC和IgE阳性MC均显著增多,且在支气管相关淋巴组织增生的区域上述肺内分泌细胞、神经纤维和IgE阳性细胞增多尤为显著,S-100蛋白和NSE阳性神经纤维分布於弥散淋巴组织和BALT中,MC与S-100蛋白阳性神经纤维紧密接触,MC表面有IgE阳性环状带,MC和IgE阳性细胞出现在支气管上皮间和肺泡壁。

The palisade tissue cells of common type were 2 layers, while those of spur-type and columnar type were 3 or 4 layers. The ratio of palisade tissue and spongy tissue of common type was less than 1, and that of spur type and columnar type was more than 1. Moreover, the number of palisade tissue cell layers and the ratio of P/S were increased with the dwarfing degrees.

普通型苹果的叶片栅栏组织细胞一般排列成2层,其栅栏组织与海绵组织的厚度比值小于l;而柱型和短枝型的栅栏组织细胞通常排列成3层或4层,其栅栏组织与海绵组织的比值大于1,而且,随着矮化程度的加强,栅栏组织细胞层数和栅/海比有增加趋势。

The hiberarchy of sclerenchyma cell wall of BM-rice leaf was not obvious by the observation of TEM.

3通过透射电镜观察,脆性突变水稻的细胞和细胞壁结构也有较大变化:叶片厚壁组织细胞细胞壁分层不明显;茎杆薄壁组织细胞形状不规则,大小不均一;根薄壁组织细胞细胞壁表面凹凸不平,形体膨松胀大。

RESULTS: Microscopically, the lesion consisted of abundant collagen fibrils, proliferated histiocytes and numerous infiltrated plasmocytes, lymphocytes and eosinophilic granulocytes. Fusiform or polygonal histiocytes had abundant, granular, eosinophilic cytoplasm or clear cytoplasm containing radially oriented strands or resembled xanthoma cells. Emperipolesis of numerous small lymphocytes was seen in the cytoplasm of some large histiocytes.

结果: 镜下可见丰富的胶原纤维和组织细胞增生以及大量浆细胞、淋巴细胞和嗜酸粒细胞浸润,组织细胞呈梭形、多边形,胞质嗜酸性颗粒状、放射条纹状或泡沫状,一些大组织细胞质内有多量小淋巴细胞。

Results The number of cellular apoptosis was obviously increased in multiple organs from the six patients died from SARS. The cellular apoptosis occurred predominantly in cytokeratin-positive pneumoncytes, terminal bronchiolar epithelium, CD3 + and CD8 + lymphocytes, as well as a part of CD20 + lymphocytes and CD68 + macrophages.

结果 与正常组织比较,SARS患者全身多器官组织细胞凋亡数目明显增多,尤以肺脏和免疫器官为甚,主要的凋亡细胞类型为CK阳性肺泡上皮细胞、终末支气管黏膜上皮细胞、CD3+细胞、CD8+细胞,以及少部分CD2 0 +细胞和CD6 8+巨噬细胞。

Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.

结果:在绒毛组织中,IL-8蛋白定位于绒毛上皮细胞的细胞质内,且病例组的表达明显高于对照组;在蜕膜组织中;病例组蜕膜细胞的胞质内可见IL-8蛋白表达,且高于对照组;H-E染色可见病例组绒毛组织的滋养层变薄,细胞变性甚至坏死、嗜酸性增强,绒毛中轴纤维化程度增强;蜕膜组织中蜕膜细胞失去细胞间连接,部分蜕膜细胞解体、核消失。

General characteristics of cells, its chemical constituents ( protein, nucleic acid, carbohydrate, lipid), cell structure , cell cycle, cell division, four kinds of tissues (epithelial, connective, muscular, and nervous), organ, ten organ systems (dermal, skeletal, muscular, digestive, respiratory, circulatory, excretory, endosecretory, nervous, and reproductive).

细胞的一般特征,细胞的化学组成(蛋白质,核酸,糖类,脂类),细胞的结构,细胞周期,细胞分裂(无丝分裂,有丝分裂,减数分裂),四大组织(上皮组织,结缔组织,肌肉组织,神经组织),器官,十大系统(皮肤系统、骨骼系统、肌肉系统、消化系统、呼吸系统、循环系统、排泄系统、内分泌系统、神经系统和生殖系统)。

We found that 1 mutant EG4 cells showed typical characteristics of pluripotent stem cells which had no obvious difference with wild cells; 2 When induced by 10〓 M retinoic acid , mutant EG4 cells differentiated into adipocytes with high frequency compared with mutant cells, suggested that EGFR plays a role in adipocyte differentiation; 3 when injected into nude mice, mutant teratocarcinomas contained a large amount of connective tissues as well as skeletal muscle, while wild EG4 cells produced frequently cartilage, keratinocyte and neuroepithelium.

我们建立了稳定表达胞内区功能缺失的外源EGFR cDNA片段的EG4细胞,分析其生长分化特性,发现 1)突变型细胞可在未分化状态下维持长时间的增殖,表明EGFR对EG多能干细胞表型无明显影响;2)〓 M的维甲酸A(retinoid acid A,RA)诱导后,大部分对照组细胞分化为脂肪细胞,而突变型细胞分化为脂肪细胞的比例明显较少,表明EGFR在脂肪细胞的发育分化中具有一定的调节作用;3)畸胎瘤切片分析显示,突变型瘤组织分布有大量的未分化细胞和结缔组织,分化细胞以肌肉细胞为主;对照组瘤组织含丰富的角质上皮、软骨、神经管等依赖EGFR的分化组织。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色:翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成;基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质,在翼状胬肉体部深层基质中存在粗糙的纤维组织;正常球结膜组织细胞排列整齐;基质为疏松结缔组织,胶原纤维平行排列,其间可见成纤维细胞,散在少量中性粒细胞、毛细血管;Masson染色:翼状胬肉浅层基质中存在致密的胶原纤维染色,深层基质中的胶原纤维存在变性样改变;VG染色:翼状胬肉组织深层基质中存在大量变性的胶原纤维,其间夹杂黑色的弹性纤维;免疫组化染色法:MMP-3在翼状胬肉上皮细胞中呈强表达,成纤维细胞中呈中等强度表达,血管内皮细胞中未见表达;LN在血管壁中呈强表达,在上皮细胞基底膜中呈中等强度表达,在整个基质中未见明显表达;col Ⅲ在整个翼状胬肉基质中呈强表达。

We suppose that there is a presently undefined subfraction of stem cell population that exists in a number of tissues beyond embryonic development. We term them postembryonic pluripotent stem cells. They differ from embryonic stem cells in that they gradually lose some differentiation potentials during development and adopt some special phenotypes once within a certain tissue. They can give rise to different kinds of stem cells; therefore, help to maintain balance during development and metabolism. They provide cells not only for the damaged tissues they reside, but also for damaged tissues different from them by migration triggered by proinflammatory cytokines or growth factors.

我们认为在胚胎发育后的多种组织中都存在一类原始干细胞群体,它们和造血干细胞、神经干细胞等多能干细胞不同,后者只能分化为特定胚层的细胞,而这类原始干细胞可分化为不同胚层的组织细胞;另一方面,它们区别于胚胎干细胞,在妊娠期间会逐渐失去部分分化潜能,并且会出现一些特殊表型或者分子标志,我们称其为胚胎后亚全能干细胞,这类干细胞具有下列特性:可分化为不同胚层的细胞,分布于身体的所有组织和器官,具有相同的表型或标志;他们在胚胎期存在于一些或全部的组织和器官中,可分化为不同类型的多能干细胞并在生长发育和新陈代谢中维持这些多能干细胞保持平衡;可以参与机体自我修复和更新,不仅能分化为所在器官的组织特异性细胞,参与器官重塑,也可以在炎症因子或者生长因子的趋化作用下,远处转移修复受损组织。

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