组织细胞的

In order to test the practical usefulness of the prober LZF-I we conducted population investigation, paternity determination, tissue stability and applicable extent in fish and obtained following results. The probability of chance association between random individuals was 9.23×10-16,and this indicates that the LZF-I fingerprints are completely individualspecific The paternity probability was 0.999962, and it showed that the inheritance of alleles is in accord with the Mendelian Law. The LZF-I oligonucleotide fingerprinting probe was stable.It was very effective in detecting both individual and species specificities of fish.

通过对鱼类的群体实验、亲子鉴定实验、组织细胞的稳定性实验和鱼类种类的适用范围实验后，测得：(1)LZF-I DNA指纹探针属多位点寡核苷酸探针；(2)LZF-I DNA指纹探针在鱼类种群中的鉴别机率为9.23×10 E-16；(3)LZF-I DNA指纹探针在鱼类亲子鉴定实验中的父系概率为0.999962；(4) LZF-I DNA指纹探针，是一种稳定的，既具有个体识别能力，又具有一定种属特异性的、适用于鱼类DNA指纹图研究的基因指纹探针。

The design is based on a common natural pattern, the most effective sub-division of three dimensional space –the fundamental arrangement of organic cells and the natural formation of soap bubbles.

这个设计基于一种普遍的自然样式以及最为有效的三维空间的划分—组织细胞的基本排列以及肥皂泡的自然形态。

Elastic fibers were well-distributed,and vascular endothelial cell did not immigrate.We suggested that expression of ectogenic VEGF triggered paracrine and autocrine of VEGF of chondrocyte and co-acted with VEGF receptor 2 to enhance permeability of chondrocyte and improve internal construct of engineering cartilage,and prevent vascularize proceed.

转VEGF基因软骨细胞作为组织工程的种子细胞与pluronic F-127复合后可于裸鼠体内形成转基因组织工程软骨，与对照组相比，转VEGF基因组织工程软骨具良好的生物学特性，结构均一且与正常软骨组织相似，软骨ECM的GAG、COLⅡ、COLⅩ增多，RunX2、Sox9表达增高，细胞处于增生期的肥大状态，初步分析其原因可能是转染后外源性的VEGF持续表达触发了软骨细胞VEGF自分泌，并通过VEGFR-2作用于软骨细胞，提高了软骨细胞活性，促进其存活与增殖，但未在软骨组织内引起血管内皮细胞的迁移及小血管形成。

CD8~+ cell is the main T lymphocyte subset in spleen, and B lymphocyte mainly is IgG~+ cell, moreover the amount of these B lymphocytes could exceed CD3~+ T lymphocyte subset after 7 days. CD8~+ cell is the main T lymphocyte subset in tonsil of appendix, and B lymphocyte is IgM~+ cell, and the amount could exceed CD3~+ T lymphocytes after 35 days. After 21 days, B lymphocytes in esophago tonsil are the main IgA~+ cells and the amount exceeds CD3~+ lymphocytes. The amount of CD4~+ lymphocytes is more than CD8+ lymphocytes.4. CD3~+、CD4~+ and CD8~+ T lymphocytes in spleen mainly distribute in periarterial lymphoid sheath. However IgM~+、IgG~+ and IgA~+ cells mainly distribute in ellipsoid periarterial lymphoid sheath and germinal center. T lymphocytes in appendix tonsil mainly distribute in middle and inferior part of mucous and the B lymphocytes mainly in middle and mucous between 4~7 days. Whereafter T, B lymphocytes equably distribute in mucous. CD4~+ cells arrange tightly and mainly occupy the central part in aggregates of T lymphocytes in esophago tonsil and CD8~+ lymphocytes mainly distribute in periphery. Meanwhile B lymphocytes encircle the periphery of aggregates of T lymphocytes. The aggregates of B lymphocytes is mainly the germinal center with lots of IgM~+、IgG~+ and IgA~+ cells. Meanwihle T lymphocytes encircle the periphery of aggregates of B lymphocytes.5. There is an intimate relationship between the development of tissue structure of peripheral immune organs and lymphcytopoiesis. The maturation of tissue structure is stimulated by the immigration of lymphocytes and the mature tissue structure provides place where lymphocytes grow mature and functionate.

脾脏在21日龄时达到稳定，食管扁桃体和盲肠扁桃体均在35日龄时达到稳定；脾脏中T淋巴细胞亚群以CD8~+细胞为主，B淋巴细胞则以IgG~+细胞为主，并在7日龄后数量超过CD3~+T淋巴细胞；盲肠扁桃体中T淋巴细胞亚群以CD8~+细胞为主，B淋巴细胞以IgM~+细胞为主，并在35日龄后数量超过CD3~+T淋巴细胞；21日龄后，食管扁桃体中B淋巴细胞以IgA~+细胞为主，数量超过CD3~+细胞，CD4~+细胞的数量多于CD8~+细胞。4、在T、B淋巴细胞组织定位方面，脾脏中CD3~+、CD4~+和CD8~+T淋巴细胞主要分布在动脉周围淋巴鞘中，而IgM~+、IgG~+和IgA~+细胞主要分布在椭球周围淋巴鞘和生发中心中；4～7日龄时，盲肠扁桃体中T淋巴细胞主要分布在粘膜固有层的中下部区域，而B淋巴细胞则主要分布在中上部区域，随后各日龄T、B淋巴细胞均匀地分布在粘膜固有层中；在食管扁桃体的T淋巴细胞聚集物中，CD4~+细胞紧密排列，主要占据中央部位，CD8~+细胞主要散布在外周，同时B淋巴细胞又环绕在整个T淋巴细胞聚集物的外周；B淋巴细胞聚集物主要为生发中心，其中存在大量IgM~+、IgG~+和IgA~+细胞，同时T淋巴细胞又环绕在整个B淋巴细胞聚集物的外周。5、外周免疫器官的组织结构发育和淋巴细胞发生之间存在密切的关系，淋巴细胞迁入淋巴器官刺激组织结构的发育成熟，同时成熟的组织结构又为淋巴细胞发育成熟并行使功能活动提供场所。

The percentage of TGF-β〓 positive cell in the pancreatic cancer tissue (43.8±5.2)％ was significantly higher than that in adjacent pancreatic tissue (28.7±3.6)％, P＜0.05. The worse the cancer cells differentiated and lymphy node metastasis, the more over-expression of TGF-β〓. 2. The percentage of Tr positive cell in the pancreatic cancer tissue (41±4)％ was significantly higher than that in adjacent pancreatic tissue (23±3)％, P＜0.05. The worse the cancer cells differentiated and lymphy node metastasis, the more over-expression of Tr, but the expression of Tr protein was not correlated with lymphy node metastasis (P＞0.05). 3. The percentage of β-GCD positive cell in the pancreatic cancer tissue (62.5± 4.1)％ was significantly higher than that in adjacent pancreatic tissue (33.5±2.8)％, P＜0.05. The worse the cancer cells differentiated and lymphy node metastasis, the more over-expression of β-GCD in pancreatic cancer tissue, but the expression of β-GCD protein was not correlated with lymphy node metastasis. P＞0.05. 4. the expression of β-GCD was significantly correlated with TGF-β〓 and Tr in the pancreatic cancer tissue.

结果如下：1、胰腺癌组织TGF-β〓阳性细胞百分率(43.8±5.2)％明显高于癌旁胰腺组织(28.7±3.6)％，P＜0.05；且癌细胞分化愈差或有淋巴结转移TGF-β〓过度表达愈多。2、胰腺癌组织Tr阳性细胞百分率(41±4)％，明显高于癌旁胰腺组织(23±3)％，P＜0.05；且不同分化程度胰腺癌组织Tr表达强度不同，分化程度愈低，表达强度愈高，P＜0.05；但胰腺癌Tr表达强度与淋巴结是否转移无关，P＞0.05.3、对于胰腺癌组织TGF-β〓和Tr表达，检测胰腺癌组织(32例)β-GCD阳性细胞百分率分别为(62.5±4.1)％或(62±4)％，分别明显高于癌旁胰腺组织β-GCD阳性细胞百分率(33.5±2.8)％或(43±3)％，P＜0.05；不同分化程度胰腺癌组织β-GCD表达强度不同，分化程度越低，表达强度越高，P＜0.05；但胰腺癌组织β-GCD表达强度与淋巴结是否转移无关，P＞0.05.4、TGF-β〓、Tr和β-GCD在胰腺癌组织中的表达随着分化程度的改变，呈现一致性的关系，而且TGF-β〓与淋巴结转移有关，Tr和β-GCD与淋巴结是否转移无关。

The increase ofIAA concentration regulated the expression of cyclAt gene,and subsequentlypromoted the division of meristematic cells and thus speeded up the extension ofroots.

根部生长素浓度的变化诱导了cyclAt基因的表达，促进了根分生组织细胞的分裂，加速了根的伸展。

The results showed that NaCl stress decreased the seedlings fresh mass and dry mass significantly. Under NaCl stress, the relative contents of Na(superscript +) and Cl(superscript -) in cells increased significantly, but the increment somewhat differed with different tissues. In root and stem, epidermal cells had the greatest increment, followed by cortex cells, and stelar cells; while in leaf, epidermal cells had the greatest increment, followed by cortex cells, spongy tissue cells, and palisade tissue cells.

结果表明：NaCl能显著降低长春花幼苗的鲜质量和干质量；对根、茎和叶片横切面X射线微区分析表明，NaCl胁迫导致长春花体内各组织细胞中Na和Cl相对含量显著增加，但在各器官、组织中分布稍有不同：与对照相比，根和茎中都是表皮细胞中增加幅度最大，中柱细胞次之，皮层细胞最低；在叶片中亦是表皮细胞增加幅度最大，依次是皮层细胞、海绵组织细胞及栅栏组织细胞。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

Fissa, C. chinensis and P. massoniana under full sunlight was by 42.6%, 32.6% and 4.2% higher than that under shade, respectively. The UV-B absorption of cell wall-bound alkali extractable phenolics in leaves of C. concinna and S. superba grown under full sunlight was by 35% and 11.7% higher than that under shade, respectively. But it was lower in leaves of P. massoniana, C.

全日光强下的黄果厚壳桂和荷木叶片细胞壁碱性酚类提取物的UV-B吸收能力亦分别比遮阴下的高35%和11.7%，而马尾松、锥树和藜蒴则较遮阴下的低，可能这些树种在全日光下细胞壁UV-B吸收物质有部分转移到细胞质，以增强栅栏组织细胞的保护。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。