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On the basis that Panax Tonifying Soup Mix can effectively improve and postpone cytoarchitecture impairment in hepatic and brain tissues in subacute senile mice, in order to approach the dependablity between postponing anaplasia of senile tissue cells and the ability of cleaning oxygen free radicals, lipid peroxidatic conditions, degree of cell injury of the recipe-medicine, serum SOD activity and MDA content were selected as quantitative indexes for objectively evaluating the antioxidation of this recipe-medicine.

在明确人参养荣汤有效改善、延缓D-半乳糖所致亚急性衰老鼠肝、脑组织与细胞结构损伤的基础上,为探讨该方药延缓衰老组织细胞退行性改变与清除氧自由基能力、脂质过氧化状态,细胞损伤程度的相关性,选择血清SOD活性、MDA含量作为定量指标,旨在对该方药抗氧化作用给予客观地评价。

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列,采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC,将其转染胃癌BGC-823细胞,应用Western blot和免疫组织化学法检测yrdC蛋白表达,设对照组和转染Ad-Null组,并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

One,two and four weeks after the engraftment,five rats in each group were sacri...

其余各组未发现植入细胞的标记。结论MSCs可在损伤的大鼠肺组织中分化为肺泡上皮细胞和支气管上皮细胞。

Methods Totally 41 cases with hypopharynx carcinoma were enrolled in the study. The expression of Caspase8 protein was evaluated with flow cytometry in 41 carcinoma samples and 41 paracarcinoma tissues.

采用流式细胞术检测41例下咽癌组织和41例相应癌旁组织中的Caspase8的表达,并以15例非下咽癌患者咽部正常黏膜组织作对照。

The mice were divided into three groups: a cardiac isograft of C57BL/6 to C57BL/6 mice; b cardiac allograft of Balb/c to C57BL/6 mice; c cardiac allograft of Balb/c to C57BL/6 mice with mfgl2 polyclonal antibody treatment.

采集移植心组织标本作病理学检查,以免疫组织化学方法测定mfgl2在移植心脏组织细胞上的表达,并对mgfl2的表达进行半定量分析。

This research used Hepato-pancreas of Ctenopharyngodon idellus as experimental material to study toxicological effect of olaquindox on liver cell and pancreas exocrine cell of Ctenopharyngodon idellus, using the method of histochemisiry, primary cell culture, and immunocytochemistry, and the cells were observed by TEM and LSCM.

本研究以草鱼肝胰脏(Hepato-pancreas)为试验材料,通过原代细胞培养,组织化学,免疫荧光细胞化学等实验方法,运用透射电子显微镜,激光共聚焦扫描显微镜等手段,观察三种不同浓度的喹乙醇对体外原代培养的草鱼肝细胞和胰腺外分泌部细胞的毒理影响。

The positive expression rates of Tissue Inhibitors of Metalloproteinase-2 protein in the cases of involuting groups were significantly higher than those in proliferating group and vascular malformation groups and normal skin group (P.05). Its difference was significant. Conclusions: Matrix MetallProtinase-2 and Tissue Inhibitors of Metalloproteinase-2 may play an important role in the pathogenesis of hemangiomas through angiogenesis. They may have no effect on vascular malformation and normal skin

血管瘤退化期,TIMP-2表达明显升高,说明 TIMP-2与血管瘤纤维化、消退有关,其机制可能是一方面通过抑制MMP-2介导的基底膜降解而在内皮细胞迁移、聚集中起作用,另一方面直接抑制内皮细胞的增殖及迁移,使内皮细胞高频率调亡,细胞外基质增多,组织出现纤维化,最终导致血管瘤的退化。2、血管畸形和正常皮肤小血管中MMP-2和TIMP-2不表达或表达较弱,说明血管畸形和正常皮肤小血管中并不存在异常的血管生成,据此可以对血管性疾病进行分型、分期,并指导临床治疗。

Using immunofluorescence staining with antibodies of specific enzyme of mast celland phalloidin. MC subset was defined by sorting and measuring of flow cytomery. Distribution of secretory vacuole and microfilament in MC were showed by laser scanning confocal microscope.

利用肥大细胞的特征性蛋白酶抗体和鬼比环肽的免疫荧光标记;以流式细胞仪检测分选人皮肤组织中肥大细胞的亚型;使用激光扫描共聚焦显微镜显示肥大细胞内分泌颗粒和微丝的分布。

Using immunofluorescence staining with antibodies of specific enzyme of mast cell and phalloidin. MC subset was defined by sorting and measuring of flow cytomery. Distribution of secretory vacuole and microfilament in MC were showed by laser scanning cofoncal microscope.

分泌方法:利用肥大细胞的特征性蛋白酶抗体和鬼比环肽的免疫荧光标记;以流式细胞仪检测分选人皮肤组织中肥大细胞的亚型;使用激光扫描共聚焦显微镜显示肥大细胞内分泌颗粒和微丝的分布。

The animal model of this method system have, the characteristics of the high fat, high leptin, high Ins, and match with the characteristic of clinical and the pathologic of simple obesity. This studies showed: The acupuncture can obviously reduce the vaule of intaking food and water of the fat rats, and lower the body weight and fat; The acupuncture can urge the diameter, area, physical volume of white fat cell contract, Brown fat tissue of fat rat group surroundings vascular containing large quantity of white fat cell, the acupuncture group was less. this showed: The acupuncture enhanced the white fat cell metabolism, and promotesed the white fat cell to convert to brown fat cell; The frequency of spontaneous electric of nerve cell of pvn of the fat rat is high, the acupuncture counld depress the spontaneous electric of nerve cell of pvn, This showed: the acupuncture can depress the appetite passing to adjust function of pvn nerve; The monoaminergic neurotransmitter of inside of the fat rat brain is disfunction, the acupuncture can rectify this disfunction, and adjust level of 5-HT, and affect appetite, and improve metabolism; The level of leptin and Ins of fat rat increased high, The level of it of acupuncture group decreased, compared with the fat group p.001, This showed: the fat rats have the defeat of leptin and Ins in the body, The acupuncture can lowering this defeat, The level of leptin and Ins of the fat rat in the brain is less than the normal group, The level of leptin of the acupuncture group is high than the fat group, This showed: the acupuncture can increase the transportation of leptin from blood to brain.

本方法所制肥胖大鼠模型具有高体重、高脂、高leptin和高Ins血症的特点,符合人类单纯性肥胖病的临床和病理特征;实验研究显示:针灸能明显减少肥胖大鼠的摄水、摄食量及降低其体重、体脂;针灸能促使白色脂肪细胞直径、面积、体积缩小,减少肥胖大鼠棕色脂肪组织血管周围白色脂肪细胞数量,显示:针灸增强了白色脂肪细胞的代谢,促进了白色脂肪细胞向棕色脂肪细胞的转化:肥胖大鼠PVN神经细胞自发放电频率增加,脑内单胺递质紊乱,针灸通过抑制PVN亢奋的自发放电,调整5-HT水平,达到影响食欲,改善代谢的作用;肥胖大鼠血中leptin及Ins水平增高,与正常组大鼠比有明显差异,提示大鼠体内存在leptin及Ins抵抗,针灸后肥胖大鼠随着体重体脂的下降,血中leptin、Ins水平降低,与肥胖组比均有显著性差异,而针灸后大鼠脑内leptin增加,与肥胖组比P.05,提示:针灸具有改善leptin、Ins抵抗及增加leptin脑内转运的作用。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在,'歼灭战perfuming自己与胡努特,那麼,他来到和SAT 。

The shamrock is the symbol of Ireland and of St.

三叶草是爱尔兰和圣特里克节的标志同时它的寓意是带来幸运。3片心形叶子围绕着一根断茎,深绿色。