组织细胞的

Our study demonstrates that cardiac cells possess the ability to reestablish 3-D tissue architecture in vitro;Simulated microgravity by HARV bioreactor facilitates the metabolism and function of the 3-D cardiac cell cultures.

体外培养的乳鼠心肌细胞具有形成具有同步自律收缩的三维组织结构的能力；模拟微重力的培养环境有利于改善心肌细胞三维组织样培养物的代谢和功能。

Part I: Primary culture and purification of skeletal muscle derived satellite cells for tissue engineering applications To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique for tissue engineering applications.

第一部分：种子细胞—肌卫星细胞的原代培养及鉴定本部分通过应用改良Percoll非连续密度梯度离心法分离纯化培养新西兰兔骨骼肌干细胞—肌卫星细胞，探讨为组织工程膀胱的构建提供种子细胞的可行性。

Increase the bone marrow cell component while reduce the fat component, abnormal the caryon and cytomembrane structure of bone marrow cell and stromal cell, enrich the ribose body, mitochondrion and rough surfaced endoplasmic reticulum in cytoplasm of several organs and tissues, embody abvious improvement of heart muscle, liver, immnune organs, incretory organs and sexual gland.

增加免疫介导型再障小鼠的骨髓细胞成分，减少脂肪成分。使骨髓细胞和基质细胞的细胞核和细胞膜结构趋于正常，使多种脏器组织的细胞浆内核糖体丰富，线粒体及粗面内质网发达。对心、肝、免疫器官、内分泌器官及性腺等亦有明显的改善作用。

At histopathologic analysis, neuronal tumors are usually classified as pure neuronal cell tumors (gangliocytoma, Lhermitte-Duclos disease , central neurocytoma) and mixed neuronal-glial tumors (ganglioglioma, desmoplastic infantile ganglioglioma, dysembryoplastic neuroepithelial tumor, ganglioneuroma).

根据组织病理学的分析，神经元肿瘤通常被分为：单纯的神经元细胞肿瘤（神经节细胞瘤，Lhermitte-Duclos 病〔发育不良性小脑神经节细胞〕瘤），中枢神经细胞瘤）和混合性的神经元-胶质肿瘤（神经节神经胶质瘤，成结缔组织性婴儿节细胞胶质瘤，胚胎发育不良性神经上皮瘤，神经节瘤）。

This review focuses on the current research progress of the liver cell differentiation source, and it also discusses the advantages and disadvantages of various hepatic stem cells and nonhepatic stem cells, expecting to provide a reference for liver cell source selection during tissue engineering, an adequate and suitable source for bioartificial liver and hepatocyte transplantation and to help to treat liver diseases.

本文着重论述了目前肝脏细胞的分化来源研究进展情况，并对各种肝源性和非肝源性干细胞的优缺点进行了讨论，以期为肝组织工程选择细胞来源提供参考，为生物人工肝、肝细胞移植提供充足、合适的细胞来源，协助治疗肝脏疾病。

The human cartilages are composed of chondrocyte and extracellular matrix , the form of chondrocytes are hypertrophy and the quantity are less; the ECM of cartilage are compised of type Ⅱ collagen and proteoglycan. Articular cartilages are all hyaline with little fibers. Trauma and arthritis are the main cause of cartilage injury, the ommilayer injury ofcartilage can be recovered by marrow, but because of without stimulation mechanism, the new tissues are merely fibrocartilages, they can not be coincide with hyaline cartilage in menchanics; the purely damage of articular cartilage can not stimulate chondrocyte to regenerate because of without blood circulation, thus, the plerosis of articular catilage can not depend on the proliferation of local chondrocyte. Ever since, people tried their best to find a way to reconstruct articular cartilage.

中文题名人骨髓基质干细胞成软骨诱导及多孔复合材料作为细胞载体的体外实验研究副题名外文题名 Cartilage induction of human mesenchymal stem cells and experiment on compound porous materials as cells' scaffold in vitro 论文作者刘晓岚导师周江南学科专业外科学研究领域\研究方向学位级别博士学位授予单位中南大学学位授予日期2003 论文页码总数68页关键词骨组织工程软骨细胞骨髓基质干细胞壳聚糖高分子外消旋聚乳酸馆藏号BSLW /2003 /R68 /10 造成人体关节软骨损伤的原因主要为创伤和关节炎，关节软骨全层损伤可由于骨髓中间充质干细胞的高速增殖修复，但这种修复由于缺乏相应的刺激机制，只能形成纤维软骨，而不能形成符合关节生理、力学要求的透明软骨；单纯软骨部分损伤软骨组织内无血管，软骨细胞迁移迟缓，无法使损伤区域软骨细胞再生，因此，关节炎及关节创伤后的软骨修复不能依赖于软骨细胞的增殖和迁移。

While the tissue spaces surrounding a few blood vessels wasAl and Fg positive,no Al or Fg positive cells were observed.In antemortem injurygroup,diffuse subarachnoid hemorrhage,cerebral edema,swelling or pyknotic neu-rons could be observed.The axons showed irregular swelling and disconnection at1～3h,marked swelling and disconnection at 6h,and retraction ball at 15h whichwas more remarkable at 24h after injury.The space between myelin sheaths andaxons was increased at 3～6h after injury.Tortuous and wavelike myelin sheathswhich adhered on axons incompletely,or even peeled off could be found from 15hto 24h after injury.Perinuclear lysis of Nissl bodies began at 24h after injury.Thenumber of GFAP positive cells in cerebrum and brain-stem increased significantlyfollowed by decrease,and then increased again,but the time courses of the changesin different areas of brain were not same.Al and Fg positive neural cells,mainlysurrounded blood vessels,with diffuse or peripherally distributed positive matter incytoplasm could be observed at 0.5h after injury.The number of Al or Fg positivecells and the intensity of immunoreaction increased with the time of injury.The areaof SYN positivity in medulla oblongata and pons decreased notably 3～6h afterinjury,then return to normal levels and continued to 24h after injury.

生前损伤组，可见广泛蛛网膜下腔出血，脑组织水肿，神经细胞肿胀，晚期神经元固缩；伤后1～3h见部分神经轴突不规则增粗、断裂，伤后6h断端膨大，伤后15h可见收缩球，至伤后24h更为明显；伤后3～6h可见部分神经髓鞘与轴突之间的间隙增宽，伤后15h髓鞘明显曲折，不完全附着在轴突两侧，甚至剥脱，持续到伤后24h；核周尼氏体减少在伤后24h才开始出现；同一部位的GFAP阳性细胞数目随损伤时间发生改变，先增多（最早在伤后0.5h），达到高峰后减少，其后又有增多趋势，但不同部位的GFAP阳性细胞数目增减的时间过程不尽相同，同时，大脑中的GFAP阳性细胞数目也有改变；伤后0.5h，可在脑干组织中见到Al和Fg阳性神经细胞，主要位于血管周围，阳性物在胞浆中呈弥散性分布，但部分细胞的阳性物仅分布于靠近胞膜的胞浆中而呈环状，随损伤时间延长，阳性细胞数目增多，反应强度增加；伤后3～6h，延髓及桥脑中的SYN阳性物面积减少，其后恢复到正常水平，并持续到伤后24h。

In a giving stage after pollination,p53 homologue showed high levels in the antipodal cells,integument,immature endosperm,ovary wall,tracheary elements,and aleurone layer,while c-myc homologue showed low levels in these tissues,only before pollination showed high expression in polar nucleus.

结果发现，在授粉后的一定阶段，在反足细胞、珠被、未成熟的胚乳、子房壁、导管组织和糊粉层中，p53同源基因表达强烈，c-myc同源基因的表达相反，在授粉后的这些组织中基本不表达，而在授粉前的中央细胞的极核中表达水平较高。

The organization fixing of apoenzyme subtypes by IHC: The most increasing expressing were cPLA_2 and sPLA_2 in pancreas, they were found all over in the pancreatic acinar cells in 6h after PT, and spread out from the cells in 24h,then decreased after 72h.

用IHC技术对酶蛋白亚型组织定位方面：胰腺内的表达以sPLA_2和cPLA_2增加为主，6h时即遍布于胰腺腺泡细胞；24h时由于胰腺细胞的大量破坏，腺泡细胞外均可见sPLA_2和cPLA_2的高表达；72h后细胞内外sPLA_2和cPLA_2逐渐下降。

The cells in cross-section of leaf and stem become swollen and intercellular space of the ground tissue increass when the expression of UDPGDH gene was inhibited in tobacco. When UDPGDH gene was over expresed, opposite physiological changes were observed. These results further suggested that UDPGDH gene may play an important role in the synthesis of pectin and hemicelluloses in ramie.

初步的组织学分析表明，反义和干扰表达的转基因植株中，茎段与叶横切面细胞结构较为松散，基本组织细胞间隙增大，而在UDPGDH基因过量表达的转基因植株中则观察到相反的现象，进一步证实UDPGDH基因在苎麻半纤维素和果胶合成中所具有的重要作用。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。