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In immunohistochemistry slices of skeletal muscle injury induced by contusion, the expression conditions of MyoD protein (one molecule symbol during the differentiation of sarcoblast) in satellite cells are followings: it is expressed continuously in gastrocnemius of treated group at 24 hours、2 days and 4 days after injury positive it is expressed at 4th day in fast muscle of untreated group; it is expressed at 2th day after injury in soleus (slow muscle of treated group; it is negative in slow muscle of untreated group.

由此可见,云南白药在骨骼肌损伤后的修复过程中的作用是通过抑制炎性细胞对伤后肌肉组织的破坏作用,缩短炎症反应进程,对骨骼肌卫星细胞的增殖分化有一定的促进作用,并且该作用在快肌纤维上表现较为显著,从而有利于损伤肌组织的修复。

Preadipocytes from epiploon of newborn calf were cultured by monolayer adherent method, and total RNA of the preadipocytes was extracted on day 4,6,8,10,12,14 and 16 post-culture, respec-tively. The abundance of ADPN mRNA and HSL mRNA was detected by real-time fluorescent quanti-tative RT-PCR during differentiation period from preadipocyte to adipocyte.

选取犊牛大网膜脂肪组织,进行牛前脂肪细胞的单层贴壁培养,在细胞接种后第4、6、8、10、12、14、16d分别提取细胞总RNA,采用实时荧光定量RT-PCR方法,检测了体外培养的犊牛前脂肪细胞不同时期ADPN基因mRNA和HSL基因mRNA的表达情况。

Researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects;2 Combining with the techniques of cell engineering (nuclear transplantation and electric fusion etc.), and through the process of culturing histiocyte of fish, cryopreservation and re-culture after thawing, carrying out somatic cell breeding of fish.

多年来,国内外学者对各种鱼类精液的冷冻保存进行了大量的系统研究,目前这项技术已达到实用水平,并日益发挥作用;二是对鱼类培养的组织细胞冷冻保存,通过鱼类细胞的培养、超低温冻存、解冻后再培养过程,结合细胞工程技术进行体细胞育种;大量的研究结果表明鱼类体细胞核具有发育的全能性,随着细胞培养技术、细胞工程技术日益发展成熟,完全具备实现鱼类物种种质长期保存的理论基础和技术条件。

This dissertation theoretically demonstrates the feasibility to achieve constant direct current-stimulation-circuitry under microampere , bring forward design scheme software and hardware system using in cellular electrical stimulation. In order to further quantitatively research on direct current-stimulation and ascertain the best intensity and density and stimulative current , the quantitative and qualitative analyse for gained diagnostic data are also been discussed in this paper.

本文正是从该理论基础出发,论证了实现微安级直流电刺激的离体组织细胞生长培养实验系统的可行性,提出了用于刺激实验的软硬件系统的开发放案,并对采集数据的定量和定性的分析作了初步的探讨,希望通过实验能够初步确定适合该组织细胞生长培养的最佳刺激电流和最佳刺激时间。

By in situ hybridization, 53BP2 mRNA expression is revealed in tumor cell nests of NPC paraffin sections. The strong positive particles localize in the nuclei of tumor cells; but slight positive signals are also found in tissue cells of chronic nasopharyngitis and connective tissue, such as lymphocytes and smooth muscle cells; There are also positive signals in the nuclei of hyperplastic squamous epithelial cells. However, 53BP1 mRNA expression is not found in nuclei of tumor cells, tissues of chronic nasopharyngitis and connective tissue.

原位杂交结果显示,石蜡切片的鼻咽癌癌巢内,可见53BP2的强阳性表达信号,信号呈紫色颗粒状分布于胞核,核仁清晰可见;而在相同条件下,在慢性鼻咽炎组织和癌旁上皮的结缔组织,也可见弱表达信号,如淋巴细胞和平滑肌细胞;在增生的鳞状上皮细胞的核内,可找到强阳性信号。53BP1在鼻咽癌、慢性鼻咽炎组织和癌旁上皮的结缔组织切片上,均无表达信号。

objective to investigate the influence of dit on the proliferation of human thyrocytes and compare dit with ki.to study a good kind of supplement to usi that was safe and effective.methods the human thyroid epithelium cells from para-adenoma normal tissues from thyroid adenoma patients were cultured in the absence or presence of 10-8~10-3mol/l dit or ki.mtt method was employed to obtain the proliferation of cultured human thyrocytes.

取甲状腺腺瘤旁正常甲状腺组织,应用原代细胞培养技术进行细胞培养,以不同浓度(10-8~10-3mol/l)的dit和ki刺激单层培养的甲状腺细胞,采用四甲基偶氮噻唑蓝法测定各组甲状腺细胞的增殖情况。

Results: At 3, 7 days post-grafting, SC survived in the lesioned area, hippocampus and ventriculus lateralis. At 14 days post-grafting, SC were migrating along ependyma, meninges and blood vessels. In the area of corpus callosum, SC were arranged like chains. At 60 days post-grafting, the number of SC was decreased and the fluorescent mark of SC was weakened. At 90 days post-grafting, the survival cells were few.

结果:3天、7天时移植的雪旺细胞大量存活于损伤区、海马、侧脑室的脉络丛组织中。14天后可观察到细胞沿软脑膜、室管膜、血管周间隙迁移,并在胼胝体部位排列成索条状。60天时雪旺细胞的标记荧光减弱,细胞密度减少。90天时已观察不到荧光。

Debris necrosis of lymph node is not the specific changes of KD. In the tuberculosis of lymph node,① debris necrosis is obvious, but it tends to be caseous nercrosis;② histiocytes, macrophages and foam cells are hyperplasia in the necrotic area or lymph sinus, moreover, epithelioid cells can be found and they tend to be form granuloma;③ numbers of neutrophils infiltrate in the necrotic area;④ the etiologic assay of acid fast bacterium tuberculosis bacterium is positive;⑤ typical clinical manifestation is insufficient.

淋巴结碎屑性坏死并非KD特有病变,诊断KD需先除外有明显碎屑性坏死的淋巴结结核病,后者主要表现:①碎屑性坏死虽明显,但趋于干酪样坏死;②坏死区内或同时在淋巴窦内,组织细胞、巨噬细胞和泡沫细胞增生,并演变为上皮样细胞和趋于肉芽肿形成;③坏死灶内、外可有数量不等的中性粒细胞浸润;④抗酸杆菌/结核杆菌病原学检测阳性;⑤缺乏KD的典型临床过程。

What was observed under a light microscope included: tumor cells were mulberry and micropapillary shaped or were of glandule tubular arrangement; there was obvious interspace between cancer nest and neighboring areas; micropapillary was empty of fiber blood vessel axes, with micropapillae floating freely in spongy spaces and separated by fibrous septa.

光镜下特征性表现为肿瘤细胞呈桑椹状、微乳头状或小腺管样排列,癌巢与周围间质形成明显的空隙,微乳头缺乏纤维血管轴心,每个微乳头细胞团和周边的纤维组织均存在无细胞的间隙样结构;瘤细胞CerbB-2、CgA和EMA。

Rabbit digestive tract were investigated by the method of immunohistochemical technique and histochemical technique (toluidine blue or neutral red staining). There was immunoreactive positive reaction in rabbit digestive tract except in esophagus part.

利用免疫组化技术和组织化学技术,对家兔消化道5-HT免疫反应阳性细胞、肥大细胞进行了研究,并通过邻片法比较探讨了2种细胞的对应关系。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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