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The study of the histopathology showed that the haemolymph in the disease crab decreased sharply, and was replaced by large amount of parasites, which was observed in muscle, heart and hepatopancreas from the disease crab. The pathological characteristics mainly appeared as cells swelling, necrosis, nucleus condensation and collaspe.

组织病理学观察发现,病蟹血淋巴急剧下降,代之以大量的寄生原虫,该寄生虫在病蟹的肝胰腺、鳃、心脏、肌肉等部位大量侵袭,并引起这些组织发生以坏死为主的变质性病变,其病理特征主要表现为:细胞肿胀、变性、坏死,某些细胞的细胞核固缩、碎裂或崩解。

Results 17β-HSD type 2 was found in the cytoplasm of tumor cells in 5.3% of cases.In adjacent non-malignant tissues,82.9% of cases were positive and the enzyme was detected in the cytoplasm of epithelial cells of the acini and ducts.

结果:2型17β-HSD在乳腺癌细胞的细胞浆中有表达,阳性率为5.3%;在癌旁非恶性乳腺组织腺泡上皮细胞以及导管上皮细胞的细胞浆中表达,阳性率为82.9%。

The expression was higher in undifferentiated NPC than in keratinizing squamous cell carcinoma and non-keratinizing carcinomas.

用免疫组织化学方法检测CD25细胞在鼻咽癌组织和鼻咽慢性炎症组织中的表达情况。并用组织原位杂交技术检测EB病毒对其表达的影响。

The myoblasts are the precursor of the maturated muscle cells, while the satellite cells are a kind of mononucleus cells which have the characteristics and function of the myoblasts. The satellite cells participate in the growth of skeletal muscle, the repair after injury of the muscle and provide nuclei to the maturated muscle for the normal alternation of cell nuclei. They are very important to the maintenance of the function of skeletal muscle.

成肌细胞为成熟肌细胞的前体细胞,而肌卫星细胞则是成熟骨骼肌组织中具有成肌细胞特征和功能的一类细胞,参与骨骼肌的生长、损伤后的修复以及为正常骨骼肌细胞核的更替提供细胞核,对骨骼肌正常功能状态的维持很重要。

Concludes:Three subsets of mast cell in human tissue are sorted and depurated with antibodies of specific enzyme and immunofluorescence labelling and flow cytometry. Mast cell of containing profuse secretary vacuoles could be displayed by confocal microscopy. It demonstrate that mast cell have material substructure offering for fast reaction of I type allergies of human being.

利用肥大细胞的特征性酶抗体、免疫荧光标记和流式细胞仪可将人组织中的肥大细胞分选纯化为三种亚型;以共聚焦显微镜显示肥大细胞含有丰富的分泌颗粒,它说明肥大细胞具备了为人体I型变态反应提供快速反应的物质基础。

Immunohistochemistry were positive for CD34 in some region where the fibroblast actively proliferated, whereas fibrocytes in mature fibrous tissue were negatively stained.

CD34免疫组织化学染色显示一些增生活跃的纤维母细胞明显表达CD34,成熟的纤维组织内的纤维细胞CD34则为阴性。

By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中,经G418筛选培养20天后再行Southern杂交检测外源〓整合情况,利用RNA狭线杂交法证明了在转录水平上表达有所提高,进一步进行了流式细胞仪测定DNA含量,使细胞在〓期产生生长阻滞作用,细胞生长速度,在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化,我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

GFAP overexpessed but NOS neuron decreased, distorted and pale. The glia in SM-DB complex of ONR hypertrophied, but NOS neuron no degeneration. The increased GFAP immunoreactivity and the morphometric and cytological changes in SM-DB complex astrocytes may reflect a sustained upregulation of cellular activity with the insults of neurons, resulting in hypertrophy of glial perikarya and cell processes.

老年减退组星形胶质细胞的细胞体密度较老年正常组和青年组明显增加(P 在GFAP免疫细胞化学和NOS组织化学双重染色的切片上,老年减退大鼠SM-DB星形胶质细胞明显肥大,突起粗壮弯曲,GFAP超量表达,而NOS阳性神经元数量减少,胞体变形,突起减少,染色深度下降。

In this essay, the culture methods of corneal epithelial cell and keratocytes have been studied. The method of rabbit corneal epithelial cells has been improved in order to solve the problem that the cells moved slowly and could not form monolayer when the traditional tissue nubbles cultivation was adopted.

本文对兔角膜上皮细胞和基质细胞的培养方法进行了研究,解决了传统角膜上皮细胞组织块贴壁培养时细胞迁出慢,不易形成单层的难题,建立了兔角膜上皮细胞原代培养的方法。

LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochchondral respiration were evaluated in each group; apoptosis was detected by flow cytometer with PI staining method and agarose gel electrophoresis; the immuneohistochemical method was used to determine the expression of bcl-2 and Caspase-3 ; the morphoiogic changes were observed with microscope.

将培养的L-6TG大鼠肌母细胞随机分为3组:①正常对照组,②缺血再灌注组(1R组),③缺血预处理组,观测了培养上清中LDH、细胞内SOD、XOD、Ca(上标 2+)含量的变化;采用MTT法检测线粒体的功能;利用流式细胞仪和细胞DNA电泳结果检测细胞凋亡情况;采用免疫组织化学的方法检测bcl-2及Caspase-3的蛋白表达情况,结合自动图像分析系统对其结果进行定量分析;在光镜下观察细胞的形态学改变。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。