组织细胞的

BACKGROUND: Seed cells and supporting materials are two key problems for cartilaginous tissue engineering.

背景：软骨组织工程的两个关键问题是种子细胞和支架材料，保证支架材料上有足够数量的功能细胞是体内形成骨和软骨组织的基础。

The positive expression of Slit2 located in the cytoplast on oligodendrocyte and astrocyte.The positive cells were found at 3d after spinal cord injury, reached peak on 7d after injury, declining after 14d. The change of Slit2 was correlative with the rehabilitation and regeneration of the axon on the forepart period.

免疫组织化学检测：Slit2蛋白定位于星型胶质细胞和少突胶质细胞的胞浆，呈棕黄色染色，于脊髓损伤后第3 d出现，阳性细胞逐渐增多，于脊髓损伤后第7 d，Slit2阳性表达的细胞数达到高峰并趋于稳定，伤后第14 d开始逐渐下降，呈一过性增高。

Through using the hot effect of covering, 60 various microelements and mineral elements are absorbed by the skin, which can prevent the cell from aging, promote the cell regenerate and metabolism of the epidermis horniness cell, as well as reduce fat and slim body.

利用包裹的热效应；60多种微量元素和矿物质敷泥膜时被皮肤吸收，可以防止细胞组织老化，促进细胞再生，表皮角质细胞的代谢，达到降脂瘦身的作用，还有作用于细胞内的酶，赋予细胞活力，肌肤富于弹性。

The research on cell immortalization may be expected to provide important insights into a broad range of cellular biological phenomenon,and the immortalized cells can play important roles in the research of cell engineering and tissue engineering as standard cells.

细胞永生化的研究有助于更广义地理解细胞生物学现象，并可作为标准细胞在细胞工程及组织工程的研究中发挥作用。

There has some disadvantages using these methods, including scarcity of donor site, keratinization and hair growth in transplanted skin, donor site morbidity.

组织工程为口腔粘膜缺损修复开辟了新途径，即应用人体少量的组织细胞经体外培养扩增后接种在支架材料上，移植到组织缺损处恢复组织的完整性。

The weight and protein content of the microsomes from different evolutionary animal and tissue cells were first determined, and the weight of microsome varies obviously and the protein content of microsome in unit is almost the same.

本文还首次系统比较了不同组织细胞和不同进化程度动物细胞的微粒体获得率及蛋白含量，得到了不同细胞微粒体获得率明显不同、单位重量微粒体蛋白含量却基本相同的结果，表明不同细胞ER含量不同，而单位ER内蛋白含量基本相同。

He type of ossein in group B was mainly type Ⅰ collagen while that in group A type Ⅱ and it exhibited as a process of fiber formation in group C. The reason for why TGF-β〓 can promote ossification induced by BMP is that can promote the differentiation and proliferation of mesenchymal cells to supply more target cells for BMP, promote the differentiation and proliferation of mature osteocytes and chondrocytes, inhibit metalloproteinase to decrease the destruction of bone tissue and promote calcification of bone matrix.

GF-〓能促进BMP骨诱导作用主要因其能促进间充质细胞的分裂增殖为BMP提供丰富的靶细胞、促进成熟骨及软骨细胞的分裂增殖、促进骨细胞产生Ⅰ型胶原而抑制软骨细胞产生Ⅱ型胶原，抑制金属蛋白酶的活性以减少组织破坏、促进基质钙化等作用而使骨生成能力增加，提示它和BMP具有协同作用。

But the chondrocytes lost primary characteristics after several passages.Conclusion A great of chondrocytes can be got from xiphoid, and the chondrocytes can grow in suspension and keep primary characteristics for a long time when they are cultured with polyHEMA in vitro. In this way, good seed cells are provided for experiment in vitro, and for the study of tissue engineering, and for clinical study.

从剑突可获得大量软骨细胞，使用PolyHEMA试剂体外培养的软骨细胞，其软骨细胞能长期以悬浮状态生长，并能使软骨细胞长期保持原有的生物学特性，为体外实验及组织工程学的研究提供更优质种子细胞，为临床的进一步研究提供理论基础。

Results NGF and its mRNA were localized in chondrocytes in the sclera, especially in the binucleate cell. In NGF and its mRNA respectively, the amount of the positive cells in the occluded eyes was significantly higher than that of the control eyes (P.05). In NGF F(subscript DG=20.556, P.001 or in its mRNA F(subscript DG=25.520, P.001, the amount of the positive cells among the occluded groups or the control groups was significantly decreased along with the time.

结果 NGF蛋白及其mRNA均表达於巩膜的软骨细胞中尤其在双核细胞中；两者在各时间段遮盖眼巩膜中阳性细胞数均明显高於对照组(P.05)；免疫组织化学F(下标 DG=20.556 P.01和原位杂交F(下标 DG=25.520 P.01在对照组各组间阳性细胞的表达数目随时间变化明显下降。

This article is divided into three parts: Part 1 Seed cells for vascular tissue engineeringPurpose: To provide seed cells for the vascular tissue engineering research.

本课题分为如下三部分：第一部分血管组织工程种子细胞的培养和鉴定目的：为血管组织工程的实验研究提供种子细胞。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐