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One eye in patients was selected to do the tear film breakup time, Schirmer test and cornea fluorescein staining, and the expression of NFκB and TGFβ1 on bulbar conjunctiva endepidermis was detected by immunohistochemistry.

TGFβ1是多功能细胞因子,对上皮细胞具有较明显的抑制作用,对成纤维细胞及其他间叶组织来源的细胞均具有明显的刺激作用,表现为胶原合成增加,有利于组织修复及伤口愈合[3,4]。

Theurinary IL-6 level positively correlated with density of glomerular matrix membrane, global sclerosis, fiber or fibrocellular crescents and interstitial fibrosis (p. 05). According to the degree of density of glomerular matrix membrane and interstitial fibrosis, urinary Col-IV level had better correlation than urinary TGF-betal and IL-6 levels.In IgAN, Col-IV showed increased expression in diseased renal tissue whereas the site of expression of TGF-betal was mainly localized within the cytoplasm of tubular epithelial cells. Interstial expressionwas also present but glomerular TGF-betal expression was found only in patients with heavy mesangial proliferation. There was a significant correlation between glomerular positivity for Col-IV and severity of histological damage. There was also a significant correlation between positivity for TGF-betal and Col-IV in the tubular epithelial and interstitial lesions. In contrast, there was no ralationship between glomerular positivity for TGF-betal and severity of histological damage.The urinary TGF-betal level paralleled tubular TGF-betal expression.

结果 ①IgAN患者尿TGF-β1、IL-6、Col-Ⅳ水平较健康人明显增高(P<0.01),该变化与血中的浓度无关(P>0.05);②尿TGF-β1水平与小管间质TGF-β1阳性表达呈正相关(P=0.000),而与小球TGF-β1阳性表达无关(P>0.05),尿Col-Ⅳ水平与小球和小管间质Col-Ⅳ阳性表达均呈良好的相关性(P<0.01),还与小管间质TGF-β1阳性表达呈正相关(P<0.05):③小球Col-Ⅳ阳性表达与肾组织慢性病变密切相关(P<0.05),小管间质Col-Ⅳ和TGF-β1阳性表达均与肾小管间质病变呈良好的相关性(P<0.01),而小球TGF-β1阳性表达与肾组织损伤无关(P>0.05);④尿TGF-β1、Col-Ⅳ水平与肾小球基质基底膜面密度、小管间质病变呈正相关(P<0.01),与小球内细胞数呈负相关(P<0.05),该结果与其在组织中的表达一致;尿IL-6水平浙江大学硕士学位论文尿TGF一B一、IL一6和Col一IV在IgA肾病中的应用价值与基质基底膜面密度、球性硬化、纤维或细胞纤维新月体所占肾小球百分数及小管间质病变均有显著的相关性(F.05);在轻度肾病理损伤时,尿'l'G卜pl、I卜6、Col一IV水平即升高,而尿Col一W在反映细胞外基质积聚和间质纤维化程度上比尿TGF一pl和IL一6有更好的相关性。

Mesenchymal stem cells are multipotent cells derived from many adult tissues, which can differentiate into cells of the mesodermal lineage, such as adipocyte, osteocyte and chondrocyte, as well as cells of other embryonic lineages. They are a promising tool for tissue engineering.

间充质干细胞是一类可从多类成体组织中分离的能向脂肪细胞、成骨细胞和软骨细胞等分化的成体干细胞,是组织工程细胞的一个理想来源。

Furthermore,the changes of cell organelles realized different cell functions, which fiber for mechanical support,parenchyma cell for preservation and vessel for transportation.3.FE-SEM and DCR technique revealed the arrangements of CMfs on cell wall of fiber, parenchyma cell and vessel.Firstly,fiber cell formed multilamellate concentric structure with thin and thick lamellae alternate.

纤维、基本薄壁组织细胞、导管分子的终端分化就是PCD;并且PCD过程是一个具有较长周期的过程,周期长短比较为基本薄壁组织细胞>纤维>导管分子;三类细胞最后的凋亡决定于细胞内原生质体完全降解的时刻。

In conclusion, features of morohopathology, IHC profile and ultrastructural findings reveal obvious divergence between CHP and HP and resemble MP, so we suggest that histogenesis of CHP is pericyte with myoid differentiation.

根据此研究之结果得知,无论在肿瘤发生部位、组织病理形态学、免疫组织化学染色结果以及超显微结构的特徵,CHP与HP具有明显的差异性,反而与MP之特徵较为相近,此结果可间接证实CHP之肿瘤细胞来源与MP的肿瘤细胞之来源较为相似,可能为血管周围细胞来源并具有类肌肉细胞的分化。

In order to understand these differences on gene transcript level, even to get the genes and their expression related to cell culture, we researched the differences of total mRNA of these tissues by RAPD-PCR, the result showed that the difference between embryo and head of nymphae was much closer than embyo with others, which was accorded to the time order of insect upgrown, and the tissue which is more different from embryo on transcript level ismore diiBcult to be cultured in vitro.

四种组织的培养难易程度和分离出的细胞形态的不同,说明存在着组织表达的差异,为了从转录水平上来认识它们的差异,甚而获得与细胞培养难易相关的基因及其表达产物,本实验还采用了RAPD分析的方法进行了这四种组织:中后期虫卵胚胎、若虫头部组织、成虫的精巢和卵巢的mRNA表达差异研究,结果表明:从发育方向上看,四种组织的表达差异有分化越大差异系数越大的趋势,而在相同条件下则越难培养。

Compared with those in control group,the number of positive immunoreactivity cell in RSR and intestine increased and its intensity of immunoreactivity enhanced obviously after rabbit was infected with Pasteurella multocida.

免疫组织化学观察发现,产抗菌肽细胞在兔圆小囊的黏膜上皮、圆顶上皮以及淋巴组织的滤泡生发中心、圆顶区和帽区均有分布;在兔感染多杀性巴氏杆菌后,圆小囊及其他肠道组织中的抗菌肽免疫组织化学阳性细胞数量明显增多,阳性反应增强。

The results demonstrated that, by comparison with un-transgenic Roselle cell or callus, the critical plating cell density and critical initiating cell density of transgenic cell line were cut down 60% and 50%, respectively,the growth cycle of transgenic cell in suspension culture was shortened from 16 days to 12 days, and the specific growth rate of transgenic Roselle callus was raised 75%, the content of flavonoid compounds in transgenic Roselle cell line or callus was hardly altered.

研究结果表明,所获得的转基因玫瑰茄细胞系的临界植板细胞密度和临界起始细胞密度分别降低了60%和50%,悬浮细胞培养周期由16d缩短到12d,转化愈伤组织的比生长速率比对照提高了75%,转基因玫瑰茄细胞和愈伤组织中的花黄素含量与对照相比没有发生明显变化。

Objective: To explore the mechanisms resulting in the recurrence of urethral scar which make urethral strictures difficult to be cured, a series experiments were conducted to find potential effective factors involved in urethral scar formation and degradation, including the studies of extracellular matrix component of urethral stricture scar, the characteristics of urethral scar fibroblast, and the effects of urine on urethral fibroblast in vitro, as well as the studies to compare the difference of collagenase activity, type Ⅰ collagen and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the tissues and cultured fibroblasts from normal urethra and strictured urethra respectively, and the studies to investigate the effect of antisense TIMP-1 oligodeoxyonucleotide on cell proliferation and collagenase activity of urethral scar fibroblast.

中文题名尿道瘢痕基础研究副题名胶原酶活性,TIMP-1的表达及其反义基因治疗外文题名 Experimental study on urethral scar-activity of collagenase,expression of TIMP-1,and antisense TIMP-1 gene transfection of urethral scar fibroblast 论文作者黄翔导师杨宇如教授学科专业外科学研究领域\研究方向学位级别博士学位授予单位四川大学学位授予日期2002 论文页码总数104页关键词尿道手术瘢痕胶原酶成纤维细胞尿道瘢痕馆藏号BSLW /2003 /R699 /12 目的:研究尿道瘢痕的细胞外基质的组成,尿道瘢痕成纤维细胞的生物学特性以及尿液对其生长的影响;比较胶原酶活性,金属蛋白酶组织抑制因子-1(TIMP-1)以及Ⅰ型胶原含量在尿道瘢痕和正常尿道组织及体外培养的成纤维细胞中的差异;研究反义TIMP-1寡核苷酸对尿道瘢痕成纤维细胞增殖以及胶原酶活性的影响。

Results VEGF-C and VEGFR-3 are all expressed on both mesenchymal cells and vascular endothelium of the embryos during early period. In middle and latter period, VEGFR-3mRNA is mainly expressed on cell plasm of alimentary canal, but it has no expression on vascular and lymphatic endothelium; The expression of VEGFR-3 is extensive and positive expression is seen on the vascular and lymphatic endothelium.

在胚胎中、后期消化道组织中VEGF-C主要表达于消化管壁组织细胞以及肠系膜内,在血管和淋巴管内皮细胞则无表达;VEGFR-3亦表现为广泛的表达,于血管和淋巴管内皮细胞可见阳性表达,并随着胚胎的发育,其在淋巴管内皮细胞的阳性表达率明显增加。

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