组织细胞

Eight kittens were deduced amblyopia by monocular chronic atropinization; four (2 normal and 2 amblyopia) kittens were perused in every three weeks. Changes in number and optical density of immunopositive neurons of the expression of VIP-protin and VIP-mRNA in the visual cortex area 17 were analyzed with immuno-cytochemistry, using the technique of in situ hybridization and SABC.

在用药后3周、6周、9周、11周分别每组中各处死两只猫，分离出视皮质17区行HE染色观察神经元形态、细胞数目以及电镜观察超微结构变化，VIP-mRNA原位杂交和VIP免疫组织化学染色观察免疫阳性细胞数以及阳性细胞平均光密度，并分别用光镜下计数和计算机图形分析软件得出结果。

There was apparent interspace between repaired tissue and surrounding normal tissues. The results indicated that: 1 Although autogenic cartilage obtained from patella in knee joint is limited, chondrocytes cultured in vitro could be regulated by some growth factors, therefore sufficieng and well-phenotype chondrocytes could be harvested to construct tissue engineering implants for transplantation. 2 It showed well therapeutic efficacy with the method of repair in our research.

实验结果表明：①自体膝关节髌骨来源的软骨虽有限，但在体外单层培养时应用生长因子对其进行调控，能为自体关节软骨缺损的移植治疗提供数量充足的、表型良好的软骨细胞以用于构建组织工程移植物；②本实验中所应用的修复方法治疗效果良好，能较有效地克服和解决目前在临床上所用的自体关节软骨细胞移植修复关节软骨缺损时所存在的软骨细胞容易丢失等问题。

Adjacent explants were connected to each other by the migrating cells with the occurrence of autorhythmic beating of the explants.

在三维胶原中，心脏组织块生长良好，迁出的细胞将相邻组织块连接起来，组织块有自发的搏动。

Cardiac explants grew well in the collagen gel. Adjacent explants were connected to each other by the migrating cells with the occurrence of autorhythmic beating of the explants.

在三维胶原中，心脏组织块生长良好，迁出的细胞将相邻组织块连接起来，组织块有自发的搏动。

Proliferating cell nuclear antigen is usually regarded as anindex of determinating cell proliferating and its expression levelin the nuclear is correlated with with the activity of cellproliferation. The activity of tumor cell proliferation can beknown by examinationing PCNA of tumor cells.

增殖细胞核抗原(proliferating cell nuclear antigen，PCNA)常作为测定细胞增殖水平的指标，其在细胞核内阳性表达的高低与细胞增殖活性密切相关，通过检测肿瘤细胞的PCNA ，可了解和评价肿瘤组织的增殖活性。

Treatment with amydae carapax decoction could resist lipid perioxidation, improve pathological function of liver tissue, improve liver function and regulate cell factor level. Thus it could inhibit hepatic stellate cells activated multiplication and extra cellular matrix synthesis and secretion, promote ECM degradation and significantly prevent and treat liver fibrosis in rats.

鳖甲煎煮液能有效地预防和治疗大鼠肝纤维化，效果显著，其作用机制可能是通过抗脂质过氧化、改善肝组织病理、改善肝功能、调控细胞因子水平等而发挥抑制肝星状细胞活化增殖及细胞外基质合成分泌、促进ECM降解吸收等综合作用，阻断和治疗肝纤维化。

Spontaneous differentiation of ESCs and iPSCs into cardiomyocytes is however limited. To elucidate the regulatory mechanisms and effectively differentiate these pluripotent cells into specific cardiomyocytes are crucial to obtain fundamental knowledge in cardiogenesis, screen drugs and promote the usage of these cells in replacement therapy. This review summarizes the current state of differentiation of cardiomyocytes from mouse and human ESCs and their regulatory factors.

但这些多能干细胞向心肌细胞自发分化的效率非常低，因此，如何有效地诱导这些多能干细胞向心肌细胞的定向分化对深入认识心肌发生发育的关键调控机制和实现其在药物发现和再生医学，如心肌梗塞、心力衰竭的细胞治疗以及心肌组织工程中的应用均具有非常重要的意义。

Spontaneous differentiation of ESCs and iPSCs into cardiomyocytes is however limited. To elucidate the regulatory mechanisms and effectively differentiate these pluripotent cells into specific cardiomyocytes are crucial to obtain fundamental knowledge in cardiogenesis, screen drugs and promote the usage of these cells in replacement therapy.

但这些多能干细胞向心肌细胞自发分化的效率非常低，因此，如何有效地诱导这些多能干细胞向心肌细胞的定向分化对深入认识心肌发生发育的关键调控机制和实现其在药物发现和再生医学，如心肌梗塞、心力衰竭的细胞治疗以及心肌组织工程中的应用均具有非常重要的意义。

Immunohistochemical stains were used to show the tryptase + mast cell and the chymase + mast cell distribution in order to observe the change of the mast cell in the model group, the control group and the treatment group.

取右下肺组织，用抗类胰蛋白酶单克隆抗体和抗类糜蛋白酶单克隆抗体免疫组织化学染色观察实验组与正常组之间肥大细胞数量的变化，HE染色观察肺组织一般病理变化。

The chymase levels were ditermined by using the method of enzyme-linked immunosorbant assay in 45 cases of chronic hepatitis. Mast cell distribution in the specimens was determined immunohistochemically by using anti-chymase antibody.

采用酶联免疫吸附实验检测45例慢性肝炎肝组织中Chymase浓度，观察不同的纤维化分期(S1-S4)，其肝组织中Chymase浓度；用免疫组织化学染色法观察Chymase单克隆抗体标记的肥大细胞分布情况。

You need to lie low for a while.

你们得歇手一段时间

If you do, you may just be pleasantly surprised.

如果你开口问了，或许有意想不到的惊喜。