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组织细胞

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Results The anesthesia range was narrowed and anesthesia duration was shortened with the prolongation of epidural catheter placement,Epidural roentgenography showed that the medium diffused normally within epidural space,biopsy of epidural tissues showed that there were inflammative cells infiltration and fiber proliferation with longer epidural catheter placement(more than 4 weeks),without significant differences between both groups.Conclusion Epidural corticosteroids can not prevent epidural adhesion from occurrance induced with long-term epidural catheter placement.

结果 随着硬膜外腔置管时间延长,麻醉阻滞范围变窄,麻醉阻滞时间也明显缩短,两组之间没有明显区别;硬膜外腔造影显示:造影剂在硬膜外腔扩散良好,无明显受阻;组织学检查显示:置管时间长(4周以上)的动物,硬膜外组织炎细胞浸润、纤维组织增生、硬膜增厚,而且随置管时间延长,这"种变化更"加显著,但两组之间没有明显区别。

The cells of B group crawled outward from the tissue inoculation in 2-3 days and the cells were polygen or rotundity and contacted each other.

B组,2-3天大部分组织块边缘有细胞移行向外长出生长晕,细胞呈多角形、圆形,细胞之间相互连接。

In the present study,we intend to establish a new model of traumatic brain-steminjury,and to study the pathological changes of neuron,axon,astrocyte,plasmaproteins,blood vessel endothelia,synapse,neurotransmitter and cytokines in rat'sand human injured brain-stem,using routing histochemistry,immunohistochemistry,in situ hybridization,LSCM and computerized image analysis.

综合现有文献,本研究拟建立新的脑干损伤动物模型,并采用常规病理组化、免疫组化、原位杂交、LSCM以及计算机图像分析等技术,对动物及人体脑干组织中的神经元、星形胶质细胞、血浆蛋白及血管内皮细胞,以及轴索、突触、神经递质及细胞因子等进行研究,并对脑干损伤的致死性病变进行初步定位,以确立对脑干损伤的鉴定和推断脑干损伤时间有意义的客观指标。

C/EBPβnuclear translocation was observed clearly in the 1-15 days silicotic tissue. There was a significant over-expression of C/EBPβin lung tissue of silica exposed rats as compared with that in the normal ones.

在染尘1-29天(以尘细胞灶和细胞性结节为主)和36-64天(以结节中成纤维细胞明显增生为主)的大鼠矽肺组织中,C/EBPβ表达的程度明显不同,差异有显著性。

Results ec was cultured and subcultured successfully. vascular endothelial cells show slabstone appearance enchased arrange under phase contrast microscope. the growth appearance was showed under sem. some dispersive high electron density of rod type weible palade body were seen under tem.

结果 培养出血管内皮细胞并传代,倒置显微镜下血管内皮细胞呈"鹅卵石"形态,扫描电镜显示"铺路石"样生长,透射电镜可见内皮细胞特征性w p小体,免疫组织化学鉴定ⅷ因子。

Methods Cell titer 96 Non-radioactive Cell Proliferation Assay was used to study the growth of cultured thyrocytes from thyroid adenomas and paraadenomatous tissues in response to various concentration of sodium iodide.

手术切取甲状腺腺瘤及腺瘤旁正常组织,采用单层细胞培养技术和非同位素标记的细胞增殖分析法,检测不同浓度碘化钠经过不同作用时间对甲状腺细胞生长的影响。

The first 0.1%collagenaseⅣ 0.1% hyaluronidase for 20 min and than 0.25% trypsin 0.04% EDTA 5μg/mL DNaseⅠfor 5 min,its enough to obtain germ cells and have optimal cell viability. 8.4±2.6×106 cells per gram testis were isolated and there was(19.7±5.3)% of PGP9.5 spermatogonium and (23.8±3.6)% of c-kit spermatogonium.

采用两步酶消化法,0.1%胶原酶和0.1%透明质酸酶消化20 min,0.25%胰酶 0.04% EDTA 5μg/mL DNaseⅠ处理5 min,就可以使睾丸生殖细胞充分分离处出来,每克睾丸组织克获得8.4±2.6×106个细胞,其中PGP9.5 精原细胞占(19.7±5.3)%,c-kit 精原细胞占总数的(23.8±3.6)%。

Sprague Dawley rat models of caudate nucleus hemorrhage were established. 10 μL of cell suspension was separately collected from the OB-OEC transplantation group and OM-OEC transplantation group, injected into rat brain tissues (1 μL/min) using a microinjector under the stereotaxis. The same volume of medium was infused into the right caudate nucleus from rats of the control group.

取SD大鼠制备尾状核出血模型,嗅球来源嗅鞘细胞移植组及嗅黏膜嗅鞘细胞移植组各取10μL细胞悬液在立体定向下引导微量注射器向大鼠脑组织内匀速注射(1 μL/min),对照组注入等量培养基,注射点为右侧尾状核。

Expressions of cytokeratin 19 and subtractive gene P02 in hepatocellular carcinoma and oval cells.

细胞角质素19及消减基因P02在肝细胞癌组织及卵圆细胞中的表达。

Combined with self-organized theory of cell ular automata and elasto - plastic theory, the elasto-plastic cell ular automata updating rule is presented.

将细胞自动机的自组织理论与弹塑性理论相结合,推导了分析平面塑性问题的细胞自动机更新规则,采用Weibull随机分布模拟岩石类材料的非均质性,元胞单元服从简单的本构关系和一定的强度准则,建立平面弹塑性细胞自动机模型,开发了相应的数值分析软件。

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