组织细胞

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列，采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC，将其转染胃癌BGC-823细胞，应用Western blot和免疫组织化学法检测yrdC蛋白表达，设对照组和转染Ad-Null组，并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

Histone deacetylase 6(HDAC6) is microtubule and HSP90 associated protein, which control genetic transcription and change cellule motor ability by deacetylased microtubule and HSP90,and then participate tumorigenesis, growth, metabasis, and the production of tumor vessel.

研究方法： 1。应用激光捕获显微切割术联合逆转录-聚合酶链反应技术，检测36例行根治性切除的原发性肝细胞癌中心处非坏死癌组织及24例正常肝组织的HDAC6 mRNA在组织中的表达，并对HDAC6与肝癌发生发展过程中相关的临床病理因素间相关性进行研究。

Methods 210 cases of MB and 9 cases of small cell tumor of cerebrum were observed with histologic and immunohistochemical techniques.

采用组织病理学和免疫组织化学方法对210例MB和9例大脑原发性小细胞肿瘤的组织形态特征和标志物表达进行比较研究。

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer .

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。

Results showed that all leaves consisted of epidermis, chlorenchyma, aquiferous tissue and vascular bundles. The leaves had the xeromorphic characteristics, including thickened epidermal cell wall, thickened cuticle, sunken stomata and well_developed aquiferous tissue. With the exception of this, there were remarkable differences in leaf structure among the six species.

结果表明，6种芦荟叶均由表皮、光合组织、储水组织和维管束组成，都表现出明显的旱生植物肉质叶的结构特征，表皮由一层扁平的细胞组成，其外壁加厚，并覆盖着厚的角质膜，气孔器凹陷，储水组织发达。

Thus, Clara cells resemble alveolar type II and bronchiolar ciliated epithelial cells by showing integrin mediated pro-migratory changes to extracellular matrix components that are present in tissues after injury.

Clara细胞与肺泡II型上皮细胞和支气管纤毛上皮细胞类似，其组织损伤后向胞外基质的促迁移改变依赖于整合素的调节。

After 7 days, some black, circular RPE cells climbed out slowly. Ten days after, cells became irregular, polygon with monolayer-coalesce in bottle bottom, which symbolized as the typical cobble-stone modality after about 13 days.

结果：组织块1d后贴壁，7d后可见有黑色、圆形的RPE细胞缓慢爬出，10d后细胞变成扁平不规则多角形，约13d后细胞基本单层融合长满瓶底，形成典型的鹅卵石样。

The thickening of the collenchyma and the sclemchyma in different degree and different ingredient are the adaptability of mechanical tissue in plant to the functions in different growth stages.

鲜明的特征。3 植物组织细胞壁结构、成分的特化与功能的适应二植物细胞细胞壁的层次及化学成分高等植物组织一般按功能主要分为保护组织。

The effects of freezing on the fermentation and speed of infusion of congou black tea was studied.Congou black tea from the fresh and withering tea leaves was performed as follows: deep freezing at -17 ℃ for 2 h,then thawing,rolling,fermenting and drying.

为了使茶多酚氧化酶以可溶形式从完整细胞或组织结构中释放出来，并保持其活性和稳定性，催化多酚类氧化，通常要将茶鲜叶在一定介质中进行充分的细胞破碎，释放出细胞内含物。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法，人肝癌细胞株Bel-7402与雷公藤甲素（10μg/mL、5μg/mL及0.25μg/mL）共培养，MTT法检测其对Bel-7402细胞增殖的影响，TUNEL技术检测其对Bel-7402细胞凋亡的影响，免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。