组织细胞

This review focuses on the current research progress of the liver cell differentiation source, and it also discusses the advantages and disadvantages of various hepatic stem cells and nonhepatic stem cells, expecting to provide a reference for liver cell source selection during tissue engineering, an adequate and suitable source for bioartificial liver and hepatocyte transplantation and to help to treat liver diseases.

本文着重论述了目前肝脏细胞的分化来源研究进展情况，并对各种肝源性和非肝源性干细胞的优缺点进行了讨论，以期为肝组织工程选择细胞来源提供参考，为生物人工肝、肝细胞移植提供充足、合适的细胞来源，协助治疗肝脏疾病。

The results showed that ACP positive reactions were mainly deposited in lysosome, nucellar cell, cellular endomembrane, mitochondriurn, and endoplasmic reticulum in four tissues of the healthy shrimp. Furthermore, ACP activity was located in microvilli, and around partly lipid droplet in liver. AKP positive reactions were mainly deposited in cell membrane, nucellar cell, lysosome. endoplasmic reticulum and cellular endomembrane in four tissues.

结果显示，在健康的对虾体内，ACP依次出现在各组织中的溶酶体、细胞核、细胞内膜、线粒体、内质网以及肝脏组织中的部分脂滴周围及微绒毛中AKP依次出现在各组织中的细胞膜、细胞核、溶酶体、内质网以及肝脏组织中的脂滴周围及微绒毛中。

All of those confirmed the odontogenetic ability of adipose-derived stem cells, offered theory and experimental basis for the abundant seed cells in tooth tissue engineering, construct new research model for the micro-environment of the odontogenesis in vitro and vivo.

目的 本研究通过酶消化法分离培养脂肪干细胞，运用牙胚细胞条件培养液体外诱导脂肪干细胞向成牙本质样细胞分化，再将脂肪干细胞与成釉器上皮细胞混合接种入体内建立牙髓-牙本质复合体模型，证实大鼠脂肪干细胞的成牙潜能，为牙齿组织工程化研究获得大量、稳定、可靠的种子细胞提供理论和实验依据；并为体内、外成牙微环境的机理研究构建新的模型。

Objective: RA is an autoimmune chronic systemic disease, characterized by inflammation, self-immune and the hyperplastic synovial pannus.

目的：类风湿关节炎(rheumatoid arthritis，RA)是一种慢性系统性疾病，其主要病理改变是滑膜炎和血管翳形成及其相关的血清组织中多种细胞因子、抗原抗体和粘附分子等的变化，而细胞因子是RA炎症和损伤的重要介质，对RA患者外周血及滑液中细胞因子改变已有大量研究，已证明多种细胞因子参与RA的病变过程。

Methods The expression of caspase-3 and PCNA was detected by tissue microarray and immunohistochemical method in 95 hepatocellular carcinoma tissues,68 parahepatic cancer tissues and 19 normal liver tissues.

应用组织芯片技术和免疫组化方法检测95例肝细胞肝癌组织、68例癌旁组织和19例正常肝组织中Caspase-3和PCNA的表达。

objective:to analyze the difference between mammary duct ectasiaand plasma cell mammitis.methods:the data of 24 cases of mde and 28 cases of pcm,including clinical manifestations,distinguished diagnoses,surgical therapy,pathological results,were analyzed.results:the clinical manifestations of mde are nipple discharge and breast mass,which should be distinguished with breast mass and early stage of breast cancer.the treatment is local resection.major pathological changes are duct ectasia and obvious periductal inflammatory changes.the clinical manifestations of pcm are breast mass and inflammatory changes,which should to be distinguished with advanced breast cancer and inflammatory breast cancer.the surgical treatment is extensive resection.the major pathological changes are inflammatory reaction and multiple abscesses in breast tissue.conclusion:mde and pcm are significant different,and they are different progressing stage of disease,thus the two diagnoses are independent.

目的：分析乳腺导管扩张症和浆细胞性乳腺炎临床上的差异。提出各自独立诊断的论据。方法：结合24例乳腺导管扩张症和28例浆细胞性乳腺炎对两病的临床症状、鉴别诊断、手术治疗、病理结果进行比较分析。结果：乳腺导管扩张症临床表现为乳头溢液和乳腺肿物，主要与乳腺肿物和早期乳腺癌鉴别诊断，手术以局部切除多见，病理表现为导管扩张及导管周围明显炎性改变。浆细胞性乳腺炎临床表现为乳腺肿物和炎性改变，主要与晚期乳腺癌和炎性乳腺癌鉴别诊断，手术切除的范围较大，病理变化以乳腺组织的炎性反应和多发性脓肿为主。结论：乳腺导管扩张症和浆细胞性乳腺炎有明显的差异，应作为这两种疾病独立诊断。

Results: Human stem cells were successfully transplanted into 6 fetal sheep and 5 lambs were born and lived. PCR analysis showed that there was human derived cell engraftment in the liver of the 5 lambs. Immunohistochemical staining and ISH with Alu probe showed human derived hepatocytes in the lobes or around periportal area, expressing albumin and CR18. Alu ISH also showed there were positive cells in the epithelia of bile duets.

结果：在移植的 6 只胎羊中，5 只顺利生产；PCR 检测显示 5 只实验羊肝脏组织中均有人源性细胞的嵌合：免疫组化及 Alu 探针原位杂交显示人源性细胞分布於门管区周围和肝小叶中，表达白蛋白、CK18 等肝细胞分化标志；Alu 探针阳胜细胞也分布於胆管上皮中。

Study on human and rabbit blood pressure, heart rate, myocardial enzyme, ESR and pathology index after ultrashort wave radiating pointed out that ultrashort wave of nothermogenesis and microthermogenesis dose had no injury on myocardial cells of human and rabbits, warmthermogenesis dose also had same effect to human, but not to rabbits. Ultrashort wave of thermogenesis dose radiating precordium for long time could seriously damage myocardial cells of human and rabbits, even bring out malignant arrhythmia and cardiac arrest.

超短波照射对新西兰白兔和人体血压、心率、心肌酶、血沉、病理组织学等指标影响的研究，说明无热量和微热量的超短波对兔和人体的心肌细胞无损伤作用；温热量超短波对兔心肌细胞有损伤作用，但对人体心肌细胞无损伤作用；长时间的热量超短波照射对心肌细胞有显著的损伤作用，甚至能导致严重的心律失常或心跳骤停。

Results After seven days of culture, some BMSCs appeared typical morphological neuronal traits. Cell bodies appeared refractile, cone-like or spherical with long processes, NSE was expressed. Animals in group A and group B recovered their motor functions in a varing degree, the difference of BBB score was significant (P.05). Hematoxylin and eosin staining demonstrated that the lesion areas of the spinal cord in group A recovered better.

结果 诱导7d后有部分细胞具备神经元样细胞形态，胞体呈锥形或圆形，有较长单极或多极的突起，有NSE阳性细胞表达；细胞移植后1~5周，A、B组动物运动功能均有不同程度恢复，但B组恢复较慢，BBB评分差异有统计学意义(P.05)；组织形态学观察示A组损伤脊髓恢复较好。

Results After seven days of culture,some BMSCs appeared typical morphological neuronal traits.Cell bodies appeared refractile,conelike or spherical with long processes,NSE was expressed.Animals in group A and group B recovered their motor functions in a varing degree,the difference of BBB score was significant(P.05).Hematoxylin and eosin staining demonstrated that the lesion areas of the spinal cord in group A recovered better.

结果 诱导7 d后有部分细胞具备神经元样细胞形态，胞体呈锥形或圆形，有较长单极或多极的突起，有NSE阳性细胞表达；细胞移植后1～5周，A、B组动物运动功能均有不同程度恢复，但B组恢复较慢，BBB评分差异有统计学意义(P.05)；组织形态学观察示A组损伤脊髓恢复较好。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。