组织细胞

In the gravid uterus, The CKs immunolabelling were detected in glandular cell, luminal epithelial cell, traphoblast cell, endoblastic cell and allantoic cell; Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus but in endoblastic cell and some luminal epithelial cell.

对分离得到的子宫内膜基质细胞和子宫内膜腺上皮细胞进行免疫组织化学标记的结果显示在体外子宫内膜基质细胞表达泛角蛋白，子宫内膜腺上皮细胞表达波形蛋白，并且这一特性不因为传代而发生丢失。

What was observed under a light microscope included: tumor cells were mulberry and micropapillary shaped or were of glandule tubular arrangement; there was obvious interspace between cancer nest and neighboring areas; micropapillary was empty of fiber blood vessel axes, with micropapillae floating freely in spongy spaces and separated by fibrous septa.

光镜下特征性表现为肿瘤细胞呈桑椹状、微乳头状或小腺管样排列，癌巢与周围间质形成明显的空隙，微乳头缺乏纤维血管轴心，每个微乳头细胞团和周边的纤维组织均存在无细胞的间隙样结构；瘤细胞CerbB-2、CgA和EMA。

Methods The expression of p15 mRNA and p15 protein was examined respectively by in suit hybridyzation and streptavidin-horseradish peroxidase immunohistochemistry method in 48 cases of esophageal carcinoma, 18 cases of esophageal dysplasia and 10 cases of incisal margin normal tissues.

采用原位杂交技术和链霉卵白素－过氧化物酶(streptavidin-horseradish peroxidase， SP)免疫组织化学染色法检测48例食管鳞状细胞癌组织，18例癌旁不典型增生组织和10例切缘正常组织中p15mRNA及其蛋白的表达水平。

Part I: Primary culture and purification of skeletal muscle derived satellite cells for tissue engineering applications To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique for tissue engineering applications.

第一部分：种子细胞—肌卫星细胞的原代培养及鉴定本部分通过应用改良Percoll非连续密度梯度离心法分离纯化培养新西兰兔骨骼肌干细胞—肌卫星细胞，探讨为组织工程膀胱的构建提供种子细胞的可行性。

Increase the bone marrow cell component while reduce the fat component, abnormal the caryon and cytomembrane structure of bone marrow cell and stromal cell, enrich the ribose body, mitochondrion and rough surfaced endoplasmic reticulum in cytoplasm of several organs and tissues, embody abvious improvement of heart muscle, liver, immnune organs, incretory organs and sexual gland.

增加免疫介导型再障小鼠的骨髓细胞成分，减少脂肪成分。使骨髓细胞和基质细胞的细胞核和细胞膜结构趋于正常，使多种脏器组织的细胞浆内核糖体丰富，线粒体及粗面内质网发达。对心、肝、免疫器官、内分泌器官及性腺等亦有明显的改善作用。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂（β-甘油磷酸钠，地塞米松，维生素C）、成脂肪细胞诱导液（地塞米松，甲基异丁酸黄嘌呤，牛胰岛素，吲哚美辛）及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化，经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物（碱性磷酸酶、骨钙素mRNA、钙结节）、脂肪标志物（脂滴、PPARγ-2mRNA）、以及类神经标志物（尼克氏体、神经烯醇化酶、神经丝蛋白）。

Methods: 5% propranolol emulsion was applied externally on the back of a guinea pig\' s ear to produce the psoriasis-like dermatitis model to observe the effects of Baibi ointment on the histopathological changes of the model.Radioimmunoassay was used to observe the levels of cytokines like IL-2、IL—6 and IL-8, and the effects of the ointment to the cytokines. MTT method was used to study the effects of the extracts of the Baibi Formula and the extracts of its main components Huangjing , Kushen , Dahuang ,Huangbai, to the multiplication of HaCaT cells—one of the Malpighian cell line with and without stimulation of TNF-α.

实验采用5%心得安乳剂建立豚鼠银屑病样皮炎模型，观察白疙软膏对豚鼠银屑病样皮炎模型组织病理改变的影响；采用放射免疫法检测豚鼠银屑病样皮炎模型皮损中细胞因子IL-2、IL-6、IL-8的水平，观察白疕软膏对豚鼠银屑病样皮炎模型皮损中细胞因子的影响；采用MTT法研究了中药白疕方提取液及白疕方主要组成药物大黄、黄柏、黄精、苦参提取液对角质形成细胞系HaCaT细胞增殖的影响及对TNF-α刺激后的角质形成细胞系HaCaT细胞增殖的影响。

Maximum tolerable dose and lowdose metronomic chemotherapy have opposite effects on the mobilization and viability of circulating endothelial progenitor cells ［J］.

在肿瘤的新生血管形成过程中，内皮细胞起决定性的作用，这些内皮细胞一部分直接来自肿瘤组织内原有血管的内皮细胞的芽生生长，一部分来自于骨髓内的内皮祖细胞的动员和释放［2］。

No callus could be induced from leaf or petiole explants of varieties F30 and Ⅱ cultured on MS medium if supplemented alone with 6-bezyladenine （6-BA） or thidiazuron, or kinetin. However, the calli were induced on MS medium containing 2.0 mg L-1 6-BA and 0.5 mg L-1 NAA （α-naphthaleneacetic acid）, and the calli derived from petioles of variety Ⅱ could produce redifferentiated shoots, but of variety F30 could not. The later could only produce shoots directly from the base of the petioles.

将品种F30和Ⅱ的叶片和带叶叶柄接种到含不同浓度单一细胞分裂素（6-BA、TDZ、KT）的MS培养基上培养，均未能诱导出愈伤组织，而在含2.0mgL-1 6-BA＋0.5mgL-1 NAA的培养基上可以诱导出愈伤组织，且由品种Ⅱ叶柄诱导产生的愈伤组织可再分化出芽，品种F30诱导的愈伤组织却不能分化，但在其叶柄基部可直接出芽。

At histopathologic analysis, neuronal tumors are usually classified as pure neuronal cell tumors (gangliocytoma, Lhermitte-Duclos disease , central neurocytoma) and mixed neuronal-glial tumors (ganglioglioma, desmoplastic infantile ganglioglioma, dysembryoplastic neuroepithelial tumor, ganglioneuroma).

根据组织病理学的分析，神经元肿瘤通常被分为：单纯的神经元细胞肿瘤（神经节细胞瘤，Lhermitte-Duclos 病〔发育不良性小脑神经节细胞〕瘤），中枢神经细胞瘤）和混合性的神经元-胶质肿瘤（神经节神经胶质瘤，成结缔组织性婴儿节细胞胶质瘤，胚胎发育不良性神经上皮瘤，神经节瘤）。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！